# HG changeset patch # User devteam # Date 1444754438 14400 # Node ID 3b0cd3132ddcac9f02c1850a517c79904629ff3a # Parent 1a20fbb8bad830976dca68bed488404e01771073 planemo upload commit 33927a87ba2eee9bf0ecdd376a66241b17b3d734 diff -r 1a20fbb8bad8 -r 3b0cd3132ddc fastx_barcode_splitter.xml --- a/fastx_barcode_splitter.xml Tue Jun 03 14:50:06 2014 -0400 +++ b/fastx_barcode_splitter.xml Tue Oct 13 12:40:38 2015 -0400 @@ -1,42 +1,38 @@ - + fastx_toolkit - fastx_barcode_splitter_galaxy_wrapper.sh $BARCODE $input "$input.name" "$output.files_path" --mismatches $mismatches --partial $partial $EOL > $output + fastx_barcode_splitter_galaxy_wrapper.sh '$BARCODE' '$input' "$input.name" "$output.files_path" --mismatches $mismatches --partial $partial $EOL > '$output' + + + + - - - + + + + - - - - + - - - - - - - - - - - - - - - - - - - - - - - + + + + + + + + + + + + + + + + + **What it does** This tool splits a Solexa library (FASTQ file) or a regular FASTA file into several files, using barcodes as the split criteria. @@ -50,11 +46,11 @@ Example:: #This line is a comment (starts with a 'number' sign) - BC1 GATCT - BC2 ATCGT - BC3 GTGAT + BC1 GATCT + BC2 ATCGT + BC3 GTGAT BC4 TGTCT - + For each barcode, a new FASTQ file will be created (with the barcode's identifier as part of the file name). Sequences matching the barcode will be stored in the appropriate file. @@ -72,7 +68,6 @@ This tool is based on `FASTX-toolkit`__ by Assaf Gordon. .. __: http://hannonlab.cshl.edu/fastx_toolkit/ - - + diff -r 1a20fbb8bad8 -r 3b0cd3132ddc fastx_barcode_splitter_galaxy_wrapper.sh --- a/fastx_barcode_splitter_galaxy_wrapper.sh Tue Jun 03 14:50:06 2014 -0400 +++ b/fastx_barcode_splitter_galaxy_wrapper.sh Tue Oct 13 12:40:38 2015 -0400 @@ -62,7 +62,7 @@ PREFIX="$BASEPATH""${LIBNAME}__" SUFFIX=".txt" -RESULTS=`zcat -f "$FASTQ_FILE" | fastx_barcode_splitter.pl --bcfile "$BARCODE_FILE" --prefix "$PREFIX" --suffix "$SUFFIX" "$@"` +RESULTS=`zcat -f < "$FASTQ_FILE" | fastx_barcode_splitter.pl --bcfile "$BARCODE_FILE" --prefix "$PREFIX" --suffix "$SUFFIX" "$@"` if [ $? != 0 ]; then echo "error" fi diff -r 1a20fbb8bad8 -r 3b0cd3132ddc tool_dependencies.xml --- a/tool_dependencies.xml Tue Jun 03 14:50:06 2014 -0400 +++ b/tool_dependencies.xml Tue Oct 13 12:40:38 2015 -0400 @@ -1,6 +1,6 @@ - +