# HG changeset patch # User devteam # Date 1380134505 14400 # Node ID 9f71775d0fe2911cb0d026b0b9c4397e25150446 Uploaded diff -r 000000000000 -r 9f71775d0fe2 fastq_quality_boxplot.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastq_quality_boxplot.xml Wed Sep 25 14:41:45 2013 -0400 @@ -0,0 +1,57 @@ + + + + fastx_toolkit + + fastq_quality_boxplot_graph.sh -t '$input.name' -i $input -o $output + + + + + + + + + + +**What it does** + +Creates a boxplot graph for the quality scores in the library. + +.. class:: infomark + +**TIP:** Use the **FASTQ Statistics** tool to generate the report file needed for this tool. + +----- + +**Output Examples** + +* Black horizontal lines are medians +* Rectangular red boxes show the Inter-quartile Range (IQR) (top value is Q3, bottom value is Q1) +* Whiskers show outlier at max. 1.5*IQR + + +An excellent quality library (median quality is 40 for almost all 36 cycles): + +.. image:: ${static_path}/fastx_icons/fastq_quality_boxplot_1.png + + +A relatively good quality library (median quality degrades towards later cycles): + +.. image:: ${static_path}/fastx_icons/fastq_quality_boxplot_2.png + +A low quality library (median drops quickly): + +.. image:: ${static_path}/fastx_icons/fastq_quality_boxplot_3.png + +------ + +This tool is based on `FASTX-toolkit`__ by Assaf Gordon. + + .. __: http://hannonlab.cshl.edu/fastx_toolkit/ + + + + + + diff -r 000000000000 -r 9f71775d0fe2 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Wed Sep 25 14:41:45 2013 -0400 @@ -0,0 +1,6 @@ + + + + + +