Mercurial > repos > devteam > fasta_clipping_histogram
comparison fasta_clipping_histogram.xml @ 4:40ec4170291e draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/fastx_toolkit/fasta_clipping_histogram commit bbb2e6b6769b03602a8ab97001f88fbec52080a1
author | iuc |
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date | Tue, 08 May 2018 10:45:30 -0400 |
parents | d0969fa24eb1 |
children | cf0a5c96c7f8 |
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3:d0969fa24eb1 | 4:40ec4170291e |
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1 <tool id="cshl_fasta_clipping_histogram" name="Length Distribution" version="1.0.0"> | 1 <tool id="cshl_fasta_clipping_histogram" name="Length Distribution" version="1.0.1"> |
2 <description>chart</description> | 2 <description>chart</description> |
3 <requirements> | 3 <macros> |
4 <requirement type="package" version="0.0.13">fastx_toolkit</requirement> | 4 <import>macros.xml</import> |
5 </requirements> | 5 </macros> |
6 <command>fasta_clipping_histogram.pl $input $outfile</command> | 6 <expand macro="requirements"> |
7 <requirement type="package" version="1.49">perl-gdgraph</requirement> | |
8 </expand> | |
9 <command detect_errors="exit_code"><![CDATA[ | |
10 fasta_clipping_histogram.pl | |
11 '$input' | |
12 '$outfile' | |
13 ]]></command> | |
7 | 14 |
8 <inputs> | 15 <inputs> |
9 <param format="fasta" name="input" type="data" label="Library to analyze" /> | 16 <expand macro="fasta_input" /> |
10 </inputs> | 17 </inputs> |
11 | 18 |
12 <outputs> | 19 <outputs> |
13 <data format="png" name="outfile" metadata_source="input" /> | 20 <data name="outfile" format="png" metadata_source="input" /> |
14 </outputs> | 21 </outputs> |
15 <tests> | 22 <tests> |
23 <test> | |
24 <param name="input" value="fasta_clipping_histogram-in1.fa" /> | |
25 <output name="outfile" file="fasta_clipping_histogram-out1.png" compare="sim_size" delta="512" /> | |
26 </test> | |
27 <test> | |
28 <param name="input" value="fasta_clipping_histogram-in2.fa" /> | |
29 <output name="outfile" file="fasta_clipping_histogram-out2.png" compare="sim_size" delta="512" /> | |
30 </test> | |
16 </tests> | 31 </tests> |
17 <help> | 32 <help><![CDATA[ |
18 **What it does** | 33 **What it does** |
19 | 34 |
20 This tool creates a histogram image of sequence lengths distribution in a given fasta dataset file. | 35 This tool creates a histogram image of sequence lengths distribution in a given fasta dataset file. |
21 | 36 |
22 **TIP:** Use this tool after clipping your library (with **FASTX Clipper tool**), to visualize the clipping results. | 37 **TIP:** Use this tool after clipping your library (with **FASTX Clipper tool**), to visualize the clipping results. |
39 | 54 |
40 **Input Formats** | 55 **Input Formats** |
41 | 56 |
42 This tool accepts short-reads FASTA files. The reads don't have to be short, but they do have to be on a single line, like so:: | 57 This tool accepts short-reads FASTA files. The reads don't have to be short, but they do have to be on a single line, like so:: |
43 | 58 |
44 >sequence1 | 59 >sequence1 |
45 AGTAGTAGGTGATGTAGAGAGAGAGAGAGTAG | 60 AGTAGTAGGTGATGTAGAGAGAGAGAGAGTAG |
46 >sequence2 | 61 >sequence2 |
47 GTGTGTGTGGGAAGTTGACACAGTA | 62 GTGTGTGTGGGAAGTTGACACAGTA |
48 >sequence3 | 63 >sequence3 |
49 CCTTGAGATTAACGCTAATCAAGTAAAC | 64 CCTTGAGATTAACGCTAATCAAGTAAAC |
50 | 65 |
51 If the sequences span over multiple lines:: | 66 If the sequences span over multiple lines:: |
52 | 67 |
53 >sequence1 | 68 >sequence1 |
54 CAGCATCTACATAATATGATCGCTATTAAACTTAAATCTCCTTGACGGAG | 69 CAGCATCTACATAATATGATCGCTATTAAACTTAAATCTCCTTGACGGAG |
55 TCTTCGGTCATAACACAAACCCAGACCTACGTATATGACAAAGCTAATAG | 70 TCTTCGGTCATAACACAAACCCAGACCTACGTATATGACAAAGCTAATAG |
56 aactggtctttacctTTAAGTTG | 71 aactggtctttacctTTAAGTTG |
57 | 72 |
58 Use the **FASTA Width Formatter** tool to re-format the FASTA into a single-lined sequences:: | 73 Use the **FASTA Width Formatter** tool to re-format the FASTA into a single-lined sequences:: |
59 | 74 |
60 >sequence1 | 75 >sequence1 |
61 CAGCATCTACATAATATGATCGCTATTAAACTTAAATCTCCTTGACGGAGTCTTCGGTCATAACACAAACCCAGACCTACGTATATGACAAAGCTAATAGaactggtctttacctTTAAGTTG | 76 CAGCATCTACATAATATGATCGCTATTAAACTTAAATCTCCTTGACGGAGTCTTCGGTCATAACACAAACCCAGACCTACGTATATGACAAAGCTAATAGaactggtctttacctTTAAGTTG |
96 | 111 |
97 ------ | 112 ------ |
98 | 113 |
99 This tool is based on `FASTX-toolkit`__ by Assaf Gordon. | 114 This tool is based on `FASTX-toolkit`__ by Assaf Gordon. |
100 | 115 |
101 .. __: http://hannonlab.cshl.edu/fastx_toolkit/ | 116 .. __: http://hannonlab.cshl.edu/fastx_toolkit/ |
102 </help> | 117 ]]></help> |
118 <expand macro="citations" /> | |
103 </tool> | 119 </tool> |