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author | devteam |
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date | Fri, 12 Aug 2016 19:17:10 -0400 |
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children | 0e2484b6829b |
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<tool id="EMBOSS: getorf42" name="getorf" version="5.0.0"> <description>Finds and extracts open reading frames (ORFs)</description> <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements> <command>getorf -sequence $input1 -outseq $out_file1 -table $table -minsize $minsize -maxsize $maxsize -find $find -methionine $methionine -circular $circular -reverse $reverse -flanking $flanking -osformat2 $out_format1 -auto</command> <inputs> <param format="fasta" name="input1" type="data"> <label>Sequences</label> </param> <param name="table" type="select"> <label>Code to use</label> <option value="0">Standard</option> <option value="1">Standard (with alternative initiation codons)</option> <option value="2">Vertebrate Mitochondrial</option> <option value="3">Yeast Mitochondrial</option> <option value="4">Mold, Protozoan, Coelenterate Mitochondrial and Mycoplasma/Spiroplasma</option> <option value="5">Invertebrate Mitochondrial</option> <option value="6">Ciliate Macronuclear and Dasycladacean</option> <option value="9">Echinoderm Mitochondrial</option> <option value="10">Euplotid Nuclear</option> <option value="11">Bacterial</option> <option value="12">Alternative Yeast Nuclear</option> <option value="13">Ascidian Mitochondrial</option> <option value="14">Flatworm Mitochondrial</option> <option value="15">Blepharisma Macronuclear</option> <option value="16">Chlorophycean Mitochondrial</option> <option value="21">Trematode Mitochondrial</option> <option value="22">Scenedesmus obliquus</option> <option value="23">Thraustochytrium Mitochondrial</option> </param> <param name="minsize" type="text" value="30"> <label>Minimum nucleotide size of ORF to report</label> </param> <param name="maxsize" type="text" value="1000000"> <label>Maximum nucleotide size of ORF to report</label> </param> <param name="find" type="select"> <label>What to output</label> <option value="0">Translation of regions between STOP codons</option> <option value="1">Translation of regions between START and STOP codons</option> <option value="2">Nucleic sequences between STOP codons</option> <option value="3">Nucleic sequences between START and STOP codons</option> <option value="4">Nucleotides flanking START codons</option> <option value="5">Nucleotides flanking initial STOP codons</option> <option value="6">Nucleotides flanking ending STOP codons</option> </param> <param name="methionine" type="select"> <label>All START codons to code for Methionine</label> <option value="yes">Yes</option> <option value="no">No</option> </param> <param name="circular" type="select"> <label>Circular sequence</label> <option value="no">No</option> <option value="yes">Yes</option> </param> <param name="reverse" type="select"> <label>Find ORFs in the reverse complement</label> <option value="yes">Yes</option> <option value="no">No</option> </param> <param name="flanking" type="text" value="100"> <label>Number of flanking nucleotides to output</label> </param> <param name="out_format1" type="select"> <label>Output Sequence File Format</label> <option value="fasta">FASTA (m)</option> <option value="acedb">ACeDB (m)</option> <option value="asn1">ASN.1 (m)</option> <option value="clustal">Clustal (m)</option> <option value="codata">CODATA (m)</option> <option value="embl">EMBL (m)</option> <option value="fitch">Fitch (m)</option> <option value="gcg">Wisconsin Package GCG 9.x and 10.x (s)</option> <option value="genbank">GENBANK (m)</option> <!-- <option value="gff">GFF (m)</option> --> <option value="hennig86">Hennig86 (m)</option> <option value="ig">Intelligenetics (m)</option> <option value="jackknifer">Jackknifer (m)</option> <option value="jackknifernon">Jackknifernon (m)</option> <option value="mega">Mega (m)</option> <option value="meganon">Meganon (m)</option> <option value="msf">Wisconsin Package GCG's MSF (m)</option> <option value="pir">NBRF (PIR) (m)</option> <option value="ncbi">NCBI style FASTA (m)</option> <option value="nexus">Nexus/PAUP (m)</option> <option value="nexusnon">Nexusnon/PAUPnon (m)</option> <option value="phylip">PHYLIP interleaved (m)</option> <option value="phylipnon">PHYLIP non-interleaved (m)</option> <option value="selex">SELEX (m)</option> <option value="staden">Staden (s)</option> <option value="strider">DNA strider (m)</option> <option value="swiss">SwisProt entry (m)</option> <option value="text">Plain sequence (s)</option> <option value="treecon">Treecon (m)</option> </param> </inputs> <outputs> <data format="fasta" name="out_file1" /> </outputs> <tests> <test> <param name="input1" value="2.fasta"/> <param name="minsize" value="30"/> <param name="maxsize" value="1000000"/> <param name="find" value="0"/> <param name="methionine" value="yes"/> <param name="circular" value="no"/> <param name="reverse" value="yes"/> <param name="table" value="0"/> <param name="flanking" value="100"/> <param name="out_format1" value="fasta"/> <output name="out_file1" file="emboss_getorf_out.fasta"/> </test> </tests> <code file="emboss_format_corrector.py" /> <help> .. class:: warningmark The input dataset needs to be sequences. ----- You can view the original documentation here_. .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/getorf.html ------ **Citation** For the underlying tool, please cite `Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 2000 Jun;16(6):276-7. <http://www.ncbi.nlm.nih.gov/pubmed/10827456>`_ If you use this tool in Galaxy, please cite `Blankenberg D, Taylor J, Schenck I, He J, Zhang Y, Ghent M, Veeraraghavan N, Albert I, Miller W, Makova KD, Hardison RC, Nekrutenko A. A framework for collaborative analysis of ENCODE data: making large-scale analyses biologist-friendly. Genome Res. 2007 Jun;17(6):960-4. <http://www.ncbi.nlm.nih.gov/pubmed/17568012>`_ </help> </tool>