Mercurial > repos > devteam > emboss_5
diff emboss_isochore.xml @ 10:9b98d3d903c6 draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/emboss_5 commit fc158bfe5f5927dc199321a2cf43310373cbc8ba
author | devteam |
---|---|
date | Fri, 12 Aug 2016 19:17:10 -0400 |
parents | |
children | 0e2484b6829b |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/emboss_isochore.xml Fri Aug 12 19:17:10 2016 -0400 @@ -0,0 +1,90 @@ +<tool id="EMBOSS: isochore47" name="isochore" version="5.0.0"> + <description>Plots isochores in large DNA sequences</description> + <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements> + <command interpreter="perl">emboss_single_outputfile_wrapper.pl isochore -sequence $input1 -outfile $ofile2 -goutfile $ofile1 -graph png -window $window -shift $shift -auto</command> + <!-- <command interpreter="perl">emboss_single_outputfile_wrapper.pl isochore -sequence $input1 -goutfile $ofile1 -graph png -window $window -shift $shift -auto</command>--> + <inputs> + <param format="fasta" name="input1" type="data"> + <label>Sequences</label> + </param> + <param name="window" type="text" value="1000"> + <label>Window size</label> + </param> + <param name="shift" type="text" value="100"> + <label>Shift increment</label> + </param> + </inputs> + <outputs> + <data format="png" name="ofile1" /> + <data format="isochore" name="ofile2" /> + </outputs> + <!-- <tests> + <test> + <param name="input1" value="2.fasta"/> + <param name="window" value="1000"/> + <param name="shift" value="100"/> + <output name="ofile1" file="emboss_isochore_out.isochore"/> + <output name="ofile2" file="emboss_isochore_out.isochore"/> + </test> + <test> + <param name="input1" value="2.fasta"/> + <param name="window" value="1000"/> + <param name="shift" value="100"/> + <output name="ofile2" file="emboss_isochore_out.isochore"/> + </test> + </tests>--> + <help> + +.. class:: warningmark + +The input dataset needs to be sequences. + +----- + +**Syntax** + +This application plots GC content over a sequence. It is intended for large sequences such as complete chromosomes or large genomic contigs, although interesting results can also be obtained from shorter sequences. You can view the original documentation here_. + + .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/isochore.html + +- Both **Window size** and **Shift increment** are intergers. + +----- + +**Example** + +- Input sequences:: + + >hg18_dna range=chrX:151073054-151073376 5'pad=0 3'pad=0 revComp=FALSE strand=? repeatMasking=none + TTTATGTCTATAATCCTTACCAAAAGTTACCTTGGAATAAGAAGAAGTCA + GTAAAAAGAAGGCTGTTGTTCCGTGAAATACTGTCTTTATGCCTCAGATT + TGGAGTGCTCAGAGCCTCTGCAGCAAAGATTTGGCATGTGTCCTAGGCCT + GCTCAGAGCAGCAAATCCCACCCTCTTGGAGAATGAGACTCATAGAGGGA + CAGCTCCCTCCTCAGAGGCTTCTCTAATGGGACTCCAAAGAGCAAACACT + CAGCCCCATGAGGACTGGCCAGGCCAAGTGGTGTGTGGGAACAGGGAGCA + GCGGTTTCCAAGAGGATACAGTA + +- Output data file:: + + Position Percent G+C 1 .. 323 + 80 0.422 + 112 0.460 + 144 0.509 + 176 0.534 + 208 0.553 + 240 0.553 + +- Output graphics file: + +.. image:: ./static/emboss_icons/isochore.png + +------ + +**Citation** + +For the underlying tool, please cite `Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 2000 Jun;16(6):276-7. <http://www.ncbi.nlm.nih.gov/pubmed/10827456>`_ + +If you use this tool in Galaxy, please cite `Blankenberg D, Taylor J, Schenck I, He J, Zhang Y, Ghent M, Veeraraghavan N, Albert I, Miller W, Makova KD, Hardison RC, Nekrutenko A. A framework for collaborative analysis of ENCODE data: making large-scale analyses biologist-friendly. Genome Res. 2007 Jun;17(6):960-4. <http://www.ncbi.nlm.nih.gov/pubmed/17568012>`_ + + </help> +</tool>