Mercurial > repos > devteam > emboss_5
diff emboss_est2genome.xml @ 10:9b98d3d903c6 draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/emboss_5 commit fc158bfe5f5927dc199321a2cf43310373cbc8ba
author | devteam |
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date | Fri, 12 Aug 2016 19:17:10 -0400 |
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children | 0e2484b6829b |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/emboss_est2genome.xml Fri Aug 12 19:17:10 2016 -0400 @@ -0,0 +1,111 @@ +<tool id="EMBOSS: est2genome32" name="est2genome" version="5.0.0"> + <description>Align EST and genomic DNA sequences</description> + <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements> + <command>est2genome -estsequence $input1 -genomesequence $input2 -outfile $out_file1 -match $match -mismatch $mismatch -gappenalty $gappenalty -intronpenalty $intronpenalty -splicepenalty + $splicepenalty -minscore $minscore -reverse $reverse -splice $splice -mode $mode -best $best -shuffle $shuffle -seed $seed -align $align -width $width -auto</command> + <inputs> + <param format="fasta" name="input1" type="data"> + <label>EST sequence(s)</label> + </param> + <param format="data" name="input2" type="data"> + <label>Genomic sequence</label> + </param> + <param name="match" type="text" value="1"> + <label>Score for matching two bases</label> + </param> + <param name="mismatch" type="text" value="1"> + <label>Cost for mismatching two bases</label> + </param> + <param name="gappenalty" type="text" value="2"> + <label>Cost for deleting a single base in either sequence, excluding introns</label> + </param> + <param name="intronpenalty" type="text" value="40"> + <label>Cost for an intron, independent of length</label> + </param> + <param name="splicepenalty" type="text" value="20"> + <label>Cost for an intron, independent of length and starting/ending on donor-acceptor sites</label> + </param> + <param name="minscore" type="text" value="30"> + <label>Exclude alignments with scores below this threshold score</label> + </param> + <param name="reverse" type="select"> + <label>Reverse the orientation of the EST sequence</label> + <option value="no">No</option> + <option value="yes">Yes</option> + </param> + <param name="splice" type="select"> + <label>Use donor and acceptor splice sites</label> + <option value="yes">Yes</option> + <option value="no">No</option> + </param> + <param name="mode" type="select"> + <label>Comparison mode</label> + <option value="both">Both strands</option> + <option value="forward">Forward strand only</option> + <option value="reverse">Reverse strand only</option> + </param> + <param name="best" type="select"> + <label>Only best comparisons</label> + <option value="yes">Yes</option> + <option value="no">No</option> + </param> + <param name="shuffle" type="text" value="0"> + <label>Shuffle</label> + </param> + <param name="seed" type="text" value="20825"> + <label>Random number seed</label> + </param> + <param name="align" type="select"> + <label>Show the alignment</label> + <option value="no">No</option> + <option value="yes">Yes</option> + </param> + <param name="width" type="text" value="50"> + <label>Alignment width</label> + </param> + </inputs> + <outputs> + <data format="est2genome" name="out_file1" /> + </outputs> + <tests> + <test> + <param name="input1" value="2.fasta"/> + <param name="input2" value="1.fasta"/> + <param name="match" value="1"/> + <param name="mismatch" value="1"/> + <param name="match" value="1"/> + <param name="gappenalty" value="2"/> + <param name="intronpenalty" value="40"/> + <param name="splicepenalty" value="20"/> + <param name="minscore" value="30"/> + <param name="reverse" value="no"/> + <param name="splice" value="yes"/> + <param name="mode" value="both"/> + <param name="best" value="yes"/> + <param name="shuffle" value="0"/> + <param name="seed" value="20825"/> + <param name="align" value="no"/> + <param name="width" value="50"/> + <output name="out_file1" file="emboss_est2genome_out.est2genome"/> + </test> + </tests> + <help> +.. class:: warningmark + +The input dataset needs to be sequences. + +----- + + You can view the original documentation here_. + + .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/est2genome.html + +------ + +**Citation** + +For the underlying tool, please cite `Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 2000 Jun;16(6):276-7. <http://www.ncbi.nlm.nih.gov/pubmed/10827456>`_ + +If you use this tool in Galaxy, please cite `Blankenberg D, Taylor J, Schenck I, He J, Zhang Y, Ghent M, Veeraraghavan N, Albert I, Miller W, Makova KD, Hardison RC, Nekrutenko A. A framework for collaborative analysis of ENCODE data: making large-scale analyses biologist-friendly. Genome Res. 2007 Jun;17(6):960-4. <http://www.ncbi.nlm.nih.gov/pubmed/17568012>`_ + </help> +</tool>