Mercurial > repos > devteam > data_manager_bwa_index_builder
view data_manager/bwa_index_builder.py @ 0:ae10d0ddbbb1 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/data_managers/data_manager_bwa_index_builder commit 86cf90107482cab1cb47fc0d42d6705f8077daa7
author | devteam |
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date | Fri, 06 Nov 2015 14:15:00 -0500 |
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#!/usr/bin/env python #Dan Blankenberg import sys import os import tempfile import optparse import subprocess from json import loads, dumps CHUNK_SIZE = 2**20 ONE_GB = 2**30 DEFAULT_DATA_TABLE_NAME = "bwa_indexes" def get_id_name( params, dbkey, fasta_description=None): #TODO: ensure sequence_id is unique and does not already appear in location file sequence_id = params['param_dict']['sequence_id'] if not sequence_id: sequence_id = dbkey sequence_name = params['param_dict']['sequence_name'] if not sequence_name: sequence_name = fasta_description if not sequence_name: sequence_name = dbkey return sequence_id, sequence_name def build_bwa_index( data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME, color_space = False ): #TODO: allow multiple FASTA input files #tmp_dir = tempfile.mkdtemp( prefix='tmp-data-manager-bwa-index-builder-' ) fasta_base_name = os.path.split( fasta_filename )[-1] sym_linked_fasta_filename = os.path.join( target_directory, fasta_base_name ) os.symlink( fasta_filename, sym_linked_fasta_filename ) if params['param_dict']['index_algorithm'] == 'automatic': if os.stat( fasta_filename ).st_size <= ONE_GB: #use 1 GB as cut off for memory vs. max of 2gb database size; this is somewhat arbitrary index_algorithm = 'is' else: index_algorithm = 'bwtsw' else: index_algorithm = params['param_dict']['index_algorithm'] args = [ 'bwa', 'index', '-a', index_algorithm ] if color_space: args.append( '-c' ) args.append( sym_linked_fasta_filename ) proc = subprocess.Popen( args=args, shell=False, cwd=target_directory ) return_code = proc.wait() if return_code: print >> sys.stderr, "Error building index." sys.exit( return_code ) data_table_entry = dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_name ) _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ) def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ): data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} ) data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] ) data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry ) return data_manager_dict def main(): #Parse Command Line parser = optparse.OptionParser() parser.add_option( '-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' ) parser.add_option( '-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' ) parser.add_option( '-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' ) parser.add_option( '-n', '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name' ) parser.add_option( '-c', '--color_space', dest='color_space', action='store_true', default=False, help='color_space' ) (options, args) = parser.parse_args() filename = args[0] params = loads( open( filename ).read() ) target_directory = params[ 'output_data' ][0]['extra_files_path'] os.mkdir( target_directory ) data_manager_dict = {} dbkey = options.fasta_dbkey if dbkey in [ None, '', '?' ]: raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) ) sequence_id, sequence_name = get_id_name( params, dbkey=dbkey, fasta_description=options.fasta_description ) #build the index build_bwa_index( data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=options.data_table_name or DEFAULT_DATA_TABLE_NAME, color_space=options.color_space ) #save info to json file open( filename, 'wb' ).write( dumps( data_manager_dict ) ) if __name__ == "__main__": main()