# HG changeset patch
# User devteam
# Date 1444754244 14400
# Node ID 6d8ab54229a02eb9d2bb723c6118fae0e7c13b13
# Parent d0d26169cc2a1ffa51c2cc3310c0d315bfc2a9d6
planemo upload commit 33927a87ba2eee9bf0ecdd376a66241b17b3d734
diff -r d0d26169cc2a -r 6d8ab54229a0 cuff_macros.xml
--- a/cuff_macros.xml Wed Nov 26 13:54:44 2014 -0500
+++ b/cuff_macros.xml Tue Oct 13 12:37:24 2015 -0400
@@ -10,8 +10,8 @@
-
-
+
+
@@ -88,4 +88,4 @@
#end for
getattr(inputs, "__len__", [].__len__)() >= 2
-
\ No newline at end of file
+
diff -r d0d26169cc2a -r 6d8ab54229a0 cuffcompare_wrapper.xml
--- a/cuffcompare_wrapper.xml Wed Nov 26 13:54:44 2014 -0500
+++ b/cuffcompare_wrapper.xml Tue Oct 13 12:37:24 2015 -0400
@@ -155,8 +155,8 @@
Cuffcompare is part of Cufflinks_. Cuffcompare helps you: (a) compare your assembled transcripts to a reference annotation and (b) track Cufflinks transcripts across multiple experiments (e.g. across a time course). Please cite: Trapnell C, Williams BA, Pertea G, Mortazavi AM, Kwan G, van Baren MJ, Salzberg SL, Wold B, Pachter L. Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms. Nature Biotechnology doi:10.1038/nbt.1621
-.. _Cufflinks: http://cufflinks.cbcb.umd.edu/
-
+.. _Cufflinks: http://cole-trapnell-lab.github.io/cufflinks/
+
------
**Know what you are doing**
@@ -165,7 +165,7 @@
There is no such thing (yet) as an automated gearshift in expression analysis. It is all like stick-shift driving in San Francisco. In other words, running this tool with default parameters will probably not give you meaningful results. A way to deal with this is to **understand** the parameters by carefully reading the `documentation`__ and experimenting. Fortunately, Galaxy makes experimenting easy.
-.. __: http://cufflinks.cbcb.umd.edu/manual.html#cuffcompare
+.. __: http://cole-trapnell-lab.github.io/cufflinks/cuffcompare/
------
@@ -225,12 +225,14 @@
2 c Contained
3 j New isoform
4 e A single exon transcript overlapping a reference exon and at least 10 bp of a reference intron, indicating a possible pre-mRNA fragment.
- 5 i A single exon transcript falling entirely with a reference intron
- 6 r Repeat. Currently determined by looking at the reference sequence and applied to transcripts where at least 50% of the bases are lower case
- 7 p Possible polymerase run-on fragment
- 8 u Unknown, intergenic transcript
- 9 o Unknown, generic overlap with reference
- 10 . (.tracking file only, indicates multiple classifications)
+ 5 i A single exon transcript falling entirely with a reference intron
+ 6 o Generic exonic overlap with a reference transcript
+ 7 p Possible polymerase run-on fragment
+ 8 r Repeat. Currently determined by looking at the soft-masked reference sequence and applied to transcripts where at least 50% of the bases are lower case
+ 9 u Unknown, intergenic transcript
+ 10 x Exonic overlap with reference on the opposite strand
+ 11 s An intron of the transfrag overlaps a reference intron on the opposite strand (likely due to read mapping errors)
+ 12 . (.tracking file only, indicates multiple classifications)
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diff -r d0d26169cc2a -r 6d8ab54229a0 shed_upload.tar.gz
Binary file shed_upload.tar.gz has changed