Mercurial > repos > devteam > bwa
annotate bwa_macros.xml @ 14:8a98cfb7ea55 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
author | iuc |
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date | Wed, 05 May 2021 18:21:27 +0000 |
parents | ceed4b724f0b |
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rev | line source |
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c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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1 <macros> |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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2 <import>read_group_macros.xml</import> |
2
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
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3 |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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4 <token name="@VERSION@">0.7.17</token> |
2
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
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5 |
11
6def3ba376a7
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 9bbf35d7f47e5ab6b78248c9907b71446d4e7b0c
iuc
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6 <token name="@pipefail@"><![CDATA[set -o | grep -q pipefail && set -o pipefail;]]></token> |
6def3ba376a7
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 9bbf35d7f47e5ab6b78248c9907b71446d4e7b0c
iuc
parents:
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7 |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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8 <token name="@set_rg_string@"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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9 #set $rg_string = "@RG\\tID:" + str($rg_id) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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10 #set $rg_string += $format_read_group("\\tSM:", $rg_sm) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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11 #set $rg_string += $format_read_group("\\tPL:", $rg_pl) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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12 #set $rg_string += $format_read_group("\\tLB:", $rg_lb) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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13 #set $rg_string += $format_read_group("\\tCN:", $rg_cn) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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14 #set $rg_string += $format_read_group("\\tDS:", $rg_ds) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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15 #set $rg_string += $format_read_group("\\tDT:", $rg_dt) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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16 #set $rg_string += $format_read_group("\\tFO:", $rg_fo) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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17 #set $rg_string += $format_read_group("\\tKS:", $rg_ks) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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18 #set $rg_string += $format_read_group("\\tPG:", $rg_pg) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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19 #set $rg_string += $format_read_group("\\tPI:", $rg_pi) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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20 #set $rg_string += $format_read_group("\\tPU:", $rg_pu) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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21 </token> |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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22 |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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23 <token name="@set_reference_fasta_filename@"><![CDATA[ |
2
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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24 #if str( $reference_source.reference_source_selector ) == "history": |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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25 #set $reference_fasta_filename = "localref.fa" |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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26 ln -s '${reference_source.ref_file}' '${reference_fasta_filename}' && |
2
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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27 bwa index |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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28 #if str($reference_source.index_a) != 'auto' |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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29 -a ${reference_source.index_a} |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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30 #end if |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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31 '${reference_fasta_filename}' && |
2
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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32 #else: |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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33 #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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changeset
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34 #end if |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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35 ]]></token> |
b4dfb5470bf3
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
devteam
parents:
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36 |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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37 <xml name="requirements"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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38 <requirements> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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39 <requirement type="package" version="@VERSION@">bwa</requirement> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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40 <requirement type="package" version="1.6">samtools</requirement> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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41 </requirements> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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42 </xml> |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
diff
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43 |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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44 <xml name="stdio"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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45 <stdio> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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46 <exit_code range="1:" /> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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47 <exit_code range=":-1" /> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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48 <regex match="Error:" /> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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49 <regex match="Exception:" /> |
9
a628f5606f68
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit a161a3a7e9149a5ba63fe20f435cd2f275dce4c8
iuc
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50 <regex match="\[bns_restore_core\] Parse error reading" /> |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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51 </stdio> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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52 </xml> |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
diff
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53 |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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54 <macro name="reference_source_conditional"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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55 <conditional name="reference_source"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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56 <param name="reference_source_selector" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options. See `Indexes` section of help below"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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57 <option value="cached">Use a built-in genome index</option> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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58 <option value="history">Use a genome from history and build index</option> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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59 </param> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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60 <when value="cached"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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61 <param name="ref_file" type="select" label="Using reference genome" help="Select genome from the list"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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62 <options from_data_table="bwa_mem_indexes"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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63 <filter type="sort_by" column="2" /> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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64 <validator type="no_options" message="No indexes are available" /> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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65 </options> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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66 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
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67 </param> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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68 </when> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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69 <when value="history"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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70 <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="You can upload a FASTA sequence to the history and use it as reference" /> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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71 <param name="index_a" type="select" label="Algorithm for constructing the BWT index" help="(-a)"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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72 <option value="auto">Auto. Let BWA decide the best algorithm to use</option> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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73 <option value="is">IS linear-time algorithm for constructing suffix array. It requires 5.37N memory where N is the size of the database. IS is moderately fast, but does not work with database larger than 2GB</option> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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74 <option value="bwtsw">BWT-SW algorithm. This method works also with big genomes</option> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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75 </param> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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76 </when> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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77 </conditional> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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78 </macro> |
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79 |
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80 <macro name="dbKeyActionsBwaMem"> |
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81 <expand macro="dbKeyActions"> |
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82 <option type="from_data_table" name="bwa_mem_indexes" column="1" offset="0"> |
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83 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> |
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84 <filter type="param_value" ref="reference_source.ref_file" column="0"/> |
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85 </option> |
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86 </expand> |
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87 </macro> |
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88 |
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89 <macro name="dbKeyActions"> |
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90 <actions> |
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91 <conditional name="reference_source.reference_source_selector"> |
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92 <when value="cached"> |
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93 <action type="metadata" name="dbkey"> |
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94 <yield /> |
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95 </action> |
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96 </when> |
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97 <when value="history"> |
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98 <action type="metadata" name="dbkey"> |
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99 <option type="from_param" name="reference_source.ref_file" param_attribute="dbkey" /> |
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100 </action> |
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101 </when> |
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102 </conditional> |
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103 </actions> |
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104 </macro> |
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105 |
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106 <token name="@RG@"> |
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107 ----- |
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108 |
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109 .. class:: warningmark |
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110 |
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111 **Read Groups are Important!** |
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112 |
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113 One of the recommended best practices in NGS analysis is adding read group information to BAM files. You can do this directly in BWA interface using the |
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114 **Specify read group information?** widget. If you are not familiar with read groups you shold know that this is effectively a way to tag reads with an additional ID. |
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115 This allows you to combine BAM files from, for example, multiple BWA runs into a single dataset. This significantly simplifies downstream processing as |
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116 instead of dealing with multiple datasets you only have to handle only one. This is possible because the read group information allows you to identify |
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117 data from different experiments even if they are combined in one file. Many downstream analysis tools such as variant callers (e.g., FreeBayes or Naive Variant Caller |
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118 present in Galaxy) are aware of read groups and will automatically generate calls for each individual sample even if they are combined within a single file. |
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119 |
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120 **Description of read groups fields** |
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121 |
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122 (from GATK FAQ webpage): |
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123 |
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124 .. csv-table:: |
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125 :header-rows: 1 |
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126 |
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127 Tag,Importance,Definition,Meaning |
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128 "ID","Required","Read group identifier. Each @RG line must have a unique ID. The value of ID is used in the RG tags of alignment records. Must be unique among all read groups in header section. Read group IDs may be modified when merging SAM files in order to handle collisions.","Ideally, this should be a globally unique identify across all sequencing data in the world, such as the Illumina flowcell + lane name and number. Will be referenced by each read with the RG:Z field, allowing tools to determine the read group information associated with each read, including the sample from which the read came. Also, a read group is effectively treated as a separate run of the NGS instrument in tools like base quality score recalibration (a GATK component) -- all reads within a read group are assumed to come from the same instrument run and to therefore share the same error model." |
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129 "SM","Sample. Use pool name where a pool is being sequenced.","Required. As important as ID.","The name of the sample sequenced in this read group. GATK tools treat all read groups with the same SM value as containing sequencing data for the same sample. Therefore it's critical that the SM field be correctly specified, especially when using multi-sample tools like the Unified Genotyper (a GATK component)." |
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130 "PL","Platform/technology used to produce the read. Valid values: ILLUMINA, SOLID, LS454, HELICOS and PACBIO.","Important. Not currently used in the GATK, but was in the past, and may return. The only way to known the sequencing technology used to generate the sequencing data","It's a good idea to use this field." |
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131 "LB","DNA preparation library identify","Essential for MarkDuplicates","MarkDuplicates uses the LB field to determine which read groups might contain molecular duplicates, in case the same DNA library was sequenced on multiple lanes." |
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132 |
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133 |
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134 **Example of Read Group usage** |
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135 |
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136 Suppose we have a trio of samples: MOM, DAD, and KID. Each has two DNA libraries prepared, one with 400 bp inserts and another with 200 bp inserts. Each of these libraries is run on two lanes of an illumina hiseq, requiring 3 x 2 x 2 = 12 lanes of data. When the data come off the sequencer, we would create 12 BAM files, with the following @RG fields in the header:: |
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137 |
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138 Dad's data: |
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139 @RG ID:FLOWCELL1.LANE1 PL:illumina LB:LIB-DAD-1 SM:DAD PI:200 |
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140 @RG ID:FLOWCELL1.LANE2 PL:illumina LB:LIB-DAD-1 SM:DAD PI:200 |
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141 @RG ID:FLOWCELL1.LANE3 PL:illumina LB:LIB-DAD-2 SM:DAD PI:400 |
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142 @RG ID:FLOWCELL1.LANE4 PL:illumina LB:LIB-DAD-2 SM:DAD PI:400 |
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143 |
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144 Mom's data: |
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145 @RG ID:FLOWCELL1.LANE5 PL:illumina LB:LIB-MOM-1 SM:MOM PI:200 |
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146 @RG ID:FLOWCELL1.LANE6 PL:illumina LB:LIB-MOM-1 SM:MOM PI:200 |
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147 @RG ID:FLOWCELL1.LANE7 PL:illumina LB:LIB-MOM-2 SM:MOM PI:400 |
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148 @RG ID:FLOWCELL1.LANE8 PL:illumina LB:LIB-MOM-2 SM:MOM PI:400 |
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149 |
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150 Kid's data: |
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151 @RG ID:FLOWCELL2.LANE1 PL:illumina LB:LIB-KID-1 SM:KID PI:200 |
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152 @RG ID:FLOWCELL2.LANE2 PL:illumina LB:LIB-KID-1 SM:KID PI:200 |
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153 @RG ID:FLOWCELL2.LANE3 PL:illumina LB:LIB-KID-2 SM:KID PI:400 |
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154 @RG ID:FLOWCELL2.LANE4 PL:illumina LB:LIB-KID-2 SM:KID PI:400 |
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155 |
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156 Note the hierarchical relationship between read groups (unique for each lane) to libraries (sequenced on two lanes) and samples (across four lanes, two lanes for each library). |
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157 </token> |
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158 <token name="@info@"> |
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159 ----- |
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160 |
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161 .. class:: infomark |
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162 |
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163 **More info** |
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164 |
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165 To obtain more information about BWA and ask questions use these resources: |
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166 |
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167 1. https://biostar.usegalaxy.org/ |
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168 2. https://www.biostars.org/ |
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169 3. https://github.com/lh3/bwa |
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170 4. http://bio-bwa.sourceforge.net/ |
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171 |
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172 </token> |
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173 </macros> |