Mercurial > repos > devteam > bwa
annotate bwa-mem.xml @ 16:47c6967dc4e0 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 8f4d108191d52e3b60212dfcc3f57541e5f849d0
author | iuc |
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date | Fri, 19 Aug 2022 18:51:11 +0000 |
parents | 22b497739c9c |
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rev | line source |
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c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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1 <?xml version="1.0"?> |
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8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
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2 <tool id="bwa_mem" name="Map with BWA-MEM" version="@VERSION@.2"> |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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3 <description>- map medium and long reads (> 100 bp) against reference genome</description> |
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22b497739c9c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
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4 <xrefs> |
22b497739c9c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
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5 <xref type="bio.tools">bwa</xref> |
22b497739c9c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
iuc
parents:
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6 </xrefs> |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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7 <macros> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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8 <import>read_group_macros.xml</import> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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9 <import>bwa_macros.xml</import> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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10 </macros> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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11 <expand macro="requirements"/> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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12 <expand macro="stdio"/> |
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29299da15326
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 4a306ebd8b7808ce963a69a3b15dd860c1fde585
iuc
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13 <command><![CDATA[ |
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6def3ba376a7
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 9bbf35d7f47e5ab6b78248c9907b71446d4e7b0c
iuc
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14 @pipefail@ |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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15 @set_reference_fasta_filename@ |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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16 |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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17 ## Begin BWA-MEM command line |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
diff
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18 |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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19 bwa mem |
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8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
parents:
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20 |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
parents:
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changeset
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21 #if str( $output_sort ) == "unsorted": |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
parents:
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22 -t 1 |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
parents:
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23 #else |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
parents:
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24 -t "\${GALAXY_SLOTS:-1}" |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
parents:
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25 #end if |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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26 ## Verbosity is set to 1 (errors only) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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27 -v 1 |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
diff
changeset
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28 |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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29 #if str( $fastq_input.fastq_input_selector ) == "paired_iv": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
changeset
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30 ## For interleaved fastq files set -p option |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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31 -p |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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32 ## check that insert statistics is used |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
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33 #if str( $fastq_input.iset_stats ): |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
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34 -I '${fastq_input.iset_stats}' |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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35 #end if |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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36 #end if |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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37 |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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38 #if str( $analysis_type.analysis_type_selector ) not in ["illumina", "full"]: |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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39 -x '$analysis_type.analysis_type_selector' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
6
diff
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40 #elif str( $analysis_type.analysis_type_selector ) == "full": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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41 ## Algorithmic options |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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diff
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42 #if str( $analysis_type.algorithmic_options.algorithmic_options_selector ) == "set": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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43 -k '${analysis_type.algorithmic_options.k}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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44 -w '${analysis_type.algorithmic_options.w}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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45 -d '${analysis_type.algorithmic_options.d}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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46 -r '${analysis_type.algorithmic_options.r}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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47 -y '${analysis_type.algorithmic_options.y}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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48 -c '${analysis_type.algorithmic_options.c}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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49 -D '${analysis_type.algorithmic_options.D}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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50 -W '${analysis_type.algorithmic_options.W}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
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51 -m '${analysis_type.algorithmic_options.m}' |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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52 ${analysis_type.algorithmic_options.S} |
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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53 ${analysis_type.algorithmic_options.P} |
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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54 ${analysis_type.algorithmic_options.e} |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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55 #end if |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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56 |
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cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
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57 ## Scoring options |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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58 #if str( $analysis_type.scoring_options.scoring_options_selector ) == "set": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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59 -A '${analysis_type.scoring_options.A}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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60 -B '${analysis_type.scoring_options.B}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
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61 -O '${analysis_type.scoring_options.O}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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62 -E '${analysis_type.scoring_options.E}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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63 -L '${analysis_type.scoring_options.L}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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64 -U '${analysis_type.scoring_options.U}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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65 #end if |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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66 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
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67 ## IO options |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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68 #if str( $analysis_type.io_options.io_options_selector ) == "set": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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69 -T '${analysis_type.io_options.T}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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70 -h '${analysis_type.io_options.h}' |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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71 ${analysis_type.io_options.a} |
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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72 ${analysis_type.io_options.C} |
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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73 ${analysis_type.io_options.V} |
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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74 ${analysis_type.io_options.Y} |
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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parents:
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75 ${analysis_type.io_options.M} |
5
b59a6178a6e0
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit dead2eaab58698fe02b8bb068f8d859eba551e8d
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76 ${analysis_type.io_options.five} |
6
a4d167793afa
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit e1191c87a0fccfafa0f23c3bd09f416a6700b387
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77 ${analysis_type.io_options.q} |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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78 #end if |
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
devteam
parents:
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79 |
8
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
iuc
parents:
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80 #end if |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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81 |
8
cbc665adcde4
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82 ## Handle read group options... |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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83 @define_read_group_helpers@ |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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84 #if str( $fastq_input.fastq_input_selector ) == "paired": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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85 #set $rg_auto_name = $read_group_name_default($fastq_input.fastq_input1, $fastq_input.fastq_input2) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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86 #else: |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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87 #set $rg_auto_name = $read_group_name_default($fastq_input.fastq_input1) |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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88 #end if |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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89 @set_use_rg_var@ |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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90 @set_read_group_vars@ |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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91 #if $use_rg |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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92 @set_rg_string@ |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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93 -R '$rg_string' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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94 #end if |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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95 |
8
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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96 #if str( $fastq_input.fastq_input_selector ) == "paired": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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97 ## check that insert statistics is used |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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98 #if str( $fastq_input.iset_stats ): |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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99 -I '${fastq_input.iset_stats}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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100 #end if |
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101 |
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102 '${reference_fasta_filename}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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103 '${fastq_input.fastq_input1}' '${fastq_input.fastq_input2}' |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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104 #elif str( $fastq_input.fastq_input_selector ) == "paired_collection": |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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105 ## check that insert statistics is used |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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106 #if str( $fastq_input.iset_stats ): |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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107 -I '${fastq_input.iset_stats}' |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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108 #end if |
c9c78719ef26
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109 |
8
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110 '${reference_fasta_filename}' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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111 '${fastq_input.fastq_input1.forward}' '${fastq_input.fastq_input1.reverse}' |
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112 #else: |
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113 '${reference_fasta_filename}' |
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114 '${fastq_input.fastq_input1}' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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115 #end if |
0
c9c78719ef26
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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116 |
14
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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117 #if str( $output_sort ) == "coordinate": |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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118 | samtools sort -@\${GALAXY_SLOTS:-2} -T "\${TMPDIR:-.}" -O bam -o '$bam_output' |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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119 #elif str( $output_sort ) == "name": |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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120 | samtools sort -n -@\${GALAXY_SLOTS:-2} -T "\${TMPDIR:-.}" -O bam -o '$bam_output' |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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121 #else |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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122 | samtools view -@ \${GALAXY_SLOTS:-2} -bS - -o '$bam_output' |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
iuc
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123 #end if |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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124 |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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125 |
10
29299da15326
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 4a306ebd8b7808ce963a69a3b15dd860c1fde585
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126 ]]></command> |
0
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planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 5a4e0ca9992af3a6e5ed2b533f04bb82ce761e0b
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127 |
8
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128 <inputs> |
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129 <expand macro="reference_source_conditional" /> |
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130 <conditional name="fastq_input"> |
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131 <param name="fastq_input_selector" type="select" label="Single or Paired-end reads" help="Select between paired and single end data"> |
cbc665adcde4
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132 <option value="paired">Paired</option> |
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133 <option value="single">Single</option> |
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134 <option value="paired_collection">Paired Collection</option> |
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135 <option value="paired_iv">Paired Interleaved</option> |
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136 </param> |
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137 <when value="paired"> |
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138 <param name="fastq_input1" type="data" format="fastqsanger,fastqsanger.gz,fasta" label="Select first set of reads" help="Specify dataset with forward reads"/> |
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139 <param name="fastq_input2" type="data" format="fastqsanger,fastqsanger.gz,fasta" label="Select second set of reads" help="Specify dataset with reverse reads"/> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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140 <param name="iset_stats" type="text" optional="True" label="Enter mean, standard deviation, max, and min for insert lengths." help="-I; This parameter is only used for paired reads. Only mean is required while sd, max, and min will be inferred. Examples: both "250" and "250,25" will work while "250,,10" will not. See below for details."> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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141 <sanitizer invalid_char=""> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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142 <valid initial="string.digits"><add value=","/> </valid> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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143 </sanitizer> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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144 </param> |
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145 </when> |
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146 <when value="single"> |
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147 <param name="fastq_input1" type="data" format="fastqsanger,fastqsanger.gz,fasta" label="Select fastq dataset" help="Specify dataset with single reads"/> |
cbc665adcde4
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148 </when> |
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149 <when value="paired_collection"> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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150 <param name="fastq_input1" format="fastqsanger,fastqsanger.gz,fasta" type="data_collection" collection_type="paired" label="Select a paired collection" help="See help section for an explanation of dataset collections"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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151 <param name="iset_stats" type="text" optional="True" label="Enter mean, standard deviation, max, and min for insert lengths." help="-I; This parameter is only used for paired reads. Only mean is required while sd, max, and min will be inferred. Examples: both "250" and "250,25" will work while "250,,10" will not. See below for details."> |
cbc665adcde4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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152 <sanitizer invalid_char=""> |
cbc665adcde4
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153 <valid initial="string.digits"><add value=","/> </valid> |
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154 </sanitizer> |
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155 </param> |
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156 </when> |
cbc665adcde4
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157 <when value="paired_iv"> |
cbc665adcde4
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158 <param name="fastq_input1" type="data" format="fastqsanger,fastqsanger.gz,fasta" label="Select fastq dataset" help="Specify dataset with interleaved reads"/> |
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159 <param name="iset_stats" type="text" optional="True" label="Enter mean, standard deviation, max, and min for insert lengths." help="-I; This parameter is only used for paired reads. Only mean is required while sd, max, and min will be inferred. Examples: both "250" and "250,25" will work while "250,,10" will not. See below for details."> |
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160 <sanitizer invalid_char=""> |
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161 <valid initial="string.digits"><add value=","/> </valid> |
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162 </sanitizer> |
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163 </param> |
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164 </when> |
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165 </conditional> |
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166 |
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167 <expand macro="read_group_conditional" /> |
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168 |
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169 <conditional name="analysis_type"> |
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170 <param name="analysis_type_selector" type="select" label="Select analysis mode"> |
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171 <option value="illumina">1.Simple Illumina mode</option> |
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172 <option value="pacbio">2.PacBio mode (-x pacbio)</option> |
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173 <option value="ont2d">3.Nanopore 2D-reads mode (-x ont2d)</option> |
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174 <option value="intractg">4.Intra-species contigs mode (-x intractg)</option> |
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175 <option value="full">5.Full list of options</option> |
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176 </param> |
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177 <when value="illumina"> |
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178 <!-- do nothing --> |
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179 </when> |
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180 <when value="pacbio"> |
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181 <!-- do nothing. all magic happens within <command> tag --> |
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182 </when> |
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183 <when value="ont2d"> |
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184 <!-- do nothing. all magic happens within <command> tag --> |
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185 </when> |
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186 <when value="intractg"> |
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187 <!-- do nothing. all magic happens within <command> tag --> |
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188 </when> |
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189 <when value="full"> |
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190 <conditional name="algorithmic_options"> |
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191 <param name="algorithmic_options_selector" type="select" label="Set algorithmic options?" help="Sets -k, -w, -d, -r, -y, -c, -D, -W, -m, -S, -P, and -e options."> |
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192 <option value="set">Set</option> |
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193 <option value="do_not_set" selected="True">Do not set</option> |
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194 </param> |
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195 <when value="set"> |
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196 <param name="k" type="integer" value="19" label="Minimum seed length" help="-k; default=19"/> |
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197 <param name="w" type="integer" value="100" label="Band width for banded alignment" help="-w; default=100"/> |
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198 <param name="d" type="integer" value="100" label="Off-diagonal X-dropoff" help="-d; default=100"/> |
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199 <param name="r" type="float" value="1.5" label="Look for internal seeds inside a seed longer than -k * THIS VALUE" help="-r; default=1.5; This is a key heuristic parameter for tuning the performance. Larger value yields fewer seeds, which leads to faster alignment speed but lower accuracy" /> |
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200 <param name="y" type="integer" value="20" label="Seed occurrence for the 3rd round seeding" help="-y; default=20" /> |
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201 <param name="c" type="integer" value="500" label="Skip seeds with more than that many occurrences" help="-c; default=500"/> |
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202 <param name="D" type="float" value="0.5" label="Drop chains shorter than this fraction of the longest overlapping chain" help="-D; default=0.5"/> |
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203 <param name="W" type="integer" value="0" label="Discard a chain if seeded bases shorter than THIS VALUE" help="-W; default=0"/> |
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204 <param name="m" type="integer" value="50" label="Perform at most this many rounds of mate rescues for each read" help="-m; default=50"/> |
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205 <param name="S" type="boolean" truevalue="-S" falsevalue="" label="Skip mate rescue" help="-S"/> |
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206 <param name="P" type="boolean" truevalue="-P" falsevalue="" label="Skip pairing; mate rescue performed unless -S also in use" help="-P"/> |
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207 <param name="e" type="boolean" truevalue="-e" falsevalue="" label="Discard full-length exact matches" help="-e"/> |
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208 </when> |
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209 <when value="do_not_set"> |
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210 <!-- do nothing --> |
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211 </when> |
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212 </conditional> |
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213 |
8
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214 <conditional name="scoring_options"> |
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215 <param name="scoring_options_selector" type="select" label="Set scoring options?" help="Sets -A, -B, -O, -E, -L, and -U options."> |
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216 <option value="set">Set</option> |
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217 <option value="do_not_set" selected="True">Do not set</option> |
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218 </param> |
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219 <when value="set"> |
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220 <param name="A" type="integer" value="1" label="Score for a sequence match" help="-A; scales options -T, -d, -B, -O, -E, -L, and -U unless overridden; default=1"/> |
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221 <param name="B" type="integer" value="4" label="Penalty for a mismatch" help="-B; default=4"/> |
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222 <param name="O" type="text" value="6,6" label="Gap open penalties for deletions and insertions" help="-O; default=6,6"> |
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223 <sanitizer invalid_char=""> |
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224 <valid initial="string.digits"><add value=","/> </valid> |
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225 </sanitizer> |
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226 </param> |
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227 <param name="E" type="text" value="1,1" label="Gap extension penalties; a gap of size k cost '-O + -E*k'. If two numbers are specified, the first is the penalty of extending a deletion and the second for extending an insertion" help="-E; default=1,1"> |
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228 <sanitizer invalid_char=""> |
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229 <valid initial="string.digits"><add value=","/> </valid> |
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230 </sanitizer> |
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231 </param> |
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232 <param name="L" type="text" value="5,5" label="Penalties for 5'-end and 3'-end clipping" help="-L; default=5,5; When performing Smith-Waterman extension, BWA-MEM keeps track of the best score reaching the end of query. If this score is larger than the best Smith-Waterman score minus the clipping penalty, clipping will not be applied. Note that in this case, the SAM AS tag reports the best Smith-Waterman score; clipping penalty is not deduced"> |
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233 <sanitizer invalid_char=""> |
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234 <valid initial="string.digits"><add value=","/> </valid> |
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235 </sanitizer> |
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236 </param> |
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237 <param name="U" type="integer" value="17" label="Penalty for an unpaired read pair" help="-U; default=17"/> |
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238 </when> |
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239 <when value="do_not_set"> |
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240 <!-- do nothing --> |
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241 </when> |
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242 </conditional> |
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243 |
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244 <conditional name="io_options"> |
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245 <param name="io_options_selector" type="select" label="Set input/output options" help="Sets -T, -h, -a, -C, -V, -Y, and -M options."> |
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246 <option value="set">Set</option> |
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247 <option value="do_not_set" selected="True">Do not set</option> |
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248 </param> |
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249 <when value="set"> |
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250 <param name="five" argument="-5" type="boolean" truevalue="-5" falsevalue="" label="For split alignment, take alignment with smallest coordinate as primary" help="Useful for HiC data"/> |
cbc665adcde4
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251 <param argument="-q" type="boolean" truevalue="-q" falsevalue="" label="Don't lower MAPQ for split alignment" help="By default the MAPQ score of a supplementary alignment will be lowered to the primary alignment score."/> |
cbc665adcde4
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252 <param name="T" type="integer" value="30" label="Minimum score to output" help="-T; default=30"/> |
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253 <param name="h" type="integer" value="5" label="If there are less than THIS VALUE hits with score >80% of the max score, output them all in the XA tag" help="-h; default=5" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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254 <param name="a" type="boolean" truevalue="-a" falsevalue="" label="Output all alignments for single-ends or unpaired paired-ends" help="-a; These alignments will be flagged as secondary alignments"/> |
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255 <param name="C" type="boolean" truevalue="-C" falsevalue="" label="Append FASTA/FASTQ comment to BAM output" help="-C"/> |
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256 <param name="V" type="boolean" truevalue="-V" falsevalue="" label="Output the reference FASTA header in the XR tag" help="-C"/> |
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257 <param name="Y" type="boolean" truevalue="-Y" falsevalue="" label="Use soft clipping for supplementary alignments" help="-Y; By default, BWA-MEM uses soft clipping for the primary alignment and hard clipping for supplementary alignments" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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258 <param name="M" type="boolean" truevalue="-M" falsevalue="" label="Mark shorter split hits of a chimeric alignment in the FLAG field as 'secondary alignment' instead of 'supplementary alignment'" help="-M; For Picard<1.96 compatibility" /> |
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259 </when> |
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260 <when value="do_not_set"> |
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261 <!-- do nothing --> |
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262 </when> |
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263 </conditional> |
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264 </when> |
0
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265 </conditional> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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266 <param name="output_sort" type="select" label="BAM sorting mode" help="The 'Not sorted' option can extend the run time of the tool significantly (cause it requires running on only a single thread)."> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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267 <option value="coordinate" selected="True">Sort by chromosomal coordinates</option> |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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268 <option value="name">Sort by read names (i.e., the QNAME field) </option> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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269 <option value="unsorted">Not sorted (sorted as input)</option> |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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270 </param> |
8
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271 </inputs> |
0
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272 |
8
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273 <outputs> |
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274 <data format="bam" name="bam_output" label="${tool.name} on ${on_string} (mapped reads in BAM format)"> |
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275 <expand macro="dbKeyActionsBwaMem" /> |
14
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276 <change_format> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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277 <when input="output_sort" value="name" format="qname_sorted.bam" /> |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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278 <when input="output_sort" value="unsorted" format="qname_input_sorted.bam" /> |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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279 </change_format> |
8
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280 </data> |
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281 </outputs> |
0
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282 |
8
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283 <tests> |
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284 <test> |
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285 <param name="reference_source_selector" value="history" /> |
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286 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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287 <param name="fastq_input_selector" value="paired"/> |
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288 <param name="fastq_input1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/> |
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289 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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290 <param name="analysis_type_selector" value="illumina"/> |
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291 <output name="bam_output" ftype="bam" file="bwa-mem-test1.bam" lines_diff="2" /> |
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292 </test> |
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293 <test> |
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294 <param name="reference_source_selector" value="history" /> |
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295 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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296 <param name="fastq_input_selector" value="single"/> |
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297 <param name="fastq_input1" ftype="fastqsanger" value="bwa-mem-fasta1.fa"/> |
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298 <param name="analysis_type_selector" value="illumina"/> |
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299 <output name="bam_output" ftype="bam" file="bwa-mem-test1-fasta.bam" lines_diff="2" /> |
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300 </test> |
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301 <test> |
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302 <param name="reference_source_selector" value="history" /> |
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303 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
cbc665adcde4
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304 <param name="fastq_input_selector" value="paired"/> |
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305 <param name="fastq_input1" ftype="fastqsanger.gz" value="bwa-mem-fastq1.fq.gz"/> |
cbc665adcde4
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306 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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307 <param name="analysis_type_selector" value="illumina"/> |
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308 <output name="bam_output" ftype="bam" file="bwa-mem-test1.bam" lines_diff="2" /> |
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309 </test> |
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310 <test> |
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311 <param name="reference_source_selector" value="history" /> |
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312 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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313 <param name="index_a" value="is"/> |
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314 <param name="fastq_input_selector" value="paired"/> |
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315 <param name="fastq_input1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/> |
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316 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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317 <param name="rg_selector" value="set"/> |
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318 <param name="ID" value="rg1"/> |
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319 <param name="PL" value="CAPILLARY"/> |
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320 <param name="LB" value="AARDVARK-1" /> |
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321 <param name="analysis_type_selector" value="illumina"/> |
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322 <output name="bam_output" ftype="bam" file="bwa-mem-test2.bam" lines_diff="2" /> |
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323 </test> |
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324 <test> |
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325 <param name="reference_source_selector" value="history" /> |
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326 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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327 <param name="fastq_input_selector" value="paired"/> |
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328 <param name="fastq_input1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/> |
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329 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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330 <param name="analysis_type_selector" value="illumina"/> |
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331 <param name="output_sort" value="unsorted"/> |
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332 <output name="bam_output" ftype="qname_input_sorted.bam" file="bwa-mem-test3.bam" lines_diff="2" /> |
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333 </test> |
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334 <test> |
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335 <param name="reference_source_selector" value="history" /> |
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336 <param name="ref_file" ftype="fasta" value="bwa-mem-mt-genome.fa"/> |
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337 <param name="fastq_input_selector" value="paired"/> |
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338 <param name="fastq_input1" ftype="fastqsanger" value="bwa-mem-fastq1.fq"/> |
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339 <param name="fastq_input2" ftype="fastqsanger" value="bwa-mem-fastq2.fq"/> |
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340 <param name="analysis_type_selector" value="illumina"/> |
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341 <param name="output_sort" value="name"/> |
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342 <output name="bam_output" ftype="qname_sorted.bam" file="bwa-mem-test4.bam" lines_diff="2" /> |
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343 </test> |
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344 </tests> |
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345 <help><![CDATA[ |
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346 **What is does** |
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347 |
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348 From http://arxiv.org/abs/1303.3997: |
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349 |
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350 BWA-MEM is an alignment algorithm for aligning sequence reads or long query sequences against a large reference genome such as human. |
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351 It automatically chooses between local and end-to-end alignments, supports paired-end reads and performs chimeric alignment. |
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352 The algorithm is robust to sequencing errors and applicable to a wide range of sequence lengths from 70bp to a few megabases. |
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353 |
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354 This Galaxy tool wraps bwa-mem module of bwa read mapping tool. The Galaxy implementation takes fastq files as input and produces output in BAM format, which can be further processed using various BAM utilities exiting in Galaxy (BAMTools, SAMTools, Picard). |
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355 |
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356 ----- |
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357 |
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358 **Indices: Selecting reference genomes for BWA** |
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359 |
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360 Galaxy wrapper for BWA allows you select between precomputed and user-defined indices for reference genomes using **Will you select a reference genome from your history or use a built-in index?** flag. This flag has two options: |
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361 |
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362 1. **Use a built-in genome index** - when selected (this is default), Galaxy provides the user with **Select reference genome index** dropdown. Genomes listed in this dropdown have been pre-indexed with bwa index utility and are ready to be mapped against. |
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363 2. **Use a genome from the history and build index** - when selected, Galaxy provides the user with **Select reference genome sequence** dropdown. This dropdown is populated by all FASTA formatted files listed in your current history. If your genome of interest is uploaded into history it will be shown there. Selecting a genome from this dropdown will cause Galaxy to first transparently index it using `bwa index` command, and then run mapping with `bwa mem`. |
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364 |
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365 If your genome of interest is not listed here you have two choices: |
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366 |
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367 1. Contact galaxy team using **Help->Support** link at the top of the interface and let us know that an index needs to be added |
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368 2. Upload your genome of interest as a FASTA file to Galaxy history and selected **Use a genome from the history and build index** option. |
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369 |
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370 ----- |
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371 |
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372 **Galaxy-specific option** |
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373 |
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374 Galaxy allows four levels of control over bwa-mem options provided by **Select analysis mode** menu option. These are: |
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375 |
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376 1. *Simple Illumina mode*: The simplest possible bwa mem application in which it alignes single or paired-end data to reference using default parameters. It is equivalent to the following command: bwa mem <reference index> <fastq dataset1> [fastq dataset2] |
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377 2. *PacBio mode*: The mode adjusted specifically for mapping of long PacBio subreads. Equivalent to the following command: bwa mem -k17 -W40 -r10 -A1 -B1 -O1 -E1 -L0 <reference index> <PacBio dataset in fastq format> |
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378 3. *Full list of options*: Allows access to all options through Galaxy interface. |
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379 |
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380 ----- |
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381 |
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382 **Bam sorting mode** |
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383 |
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384 The generated bam files can be sorted according to three criteria: coordinates, names and input order. |
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385 |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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386 In coordinate sorted mode the reads are sorted by coordinates. It means that the reads from the beginning of the first chromosome are first in the file. |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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387 |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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388 When sorted by read name, the file is sorted by the reference ID (i.e., the QNAME field). |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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389 |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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390 Finally, the *No sorted (sorted as input)* option yield a BAM file in which the records are sorted in an order corresponding to the order of the reads in the original input file. This option requires using a single thread to perform the conversion from SAM to BAM format, so the runtime is extended. |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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391 |
8a98cfb7ea55
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit 055c6c3de6c9e0f219f5792f6580244815c1cd31"
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392 |
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393 @RG@ |
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394 |
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395 @info@ |
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396 ]]></help> |
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397 <citations> |
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398 <citation type="doi">10.1093/bioinformatics/btp324</citation> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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399 <citation type="doi">10.1093/bioinformatics/btp698</citation> |
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400 <citation type="bibtex">@misc{1303.3997, |
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401 Author = {Heng Li}, |
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402 Title = {Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM}, |
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403 Year = {2013}, |
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404 Eprint = {arXiv:1303.3997}, |
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405 url = {http://arxiv.org/abs/1303.3997}, |
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406 }</citation> |
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407 </citations> |
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408 </tool> |