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     1 # bowtie2_indices.loc.sample
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     2 # This is a *.loc.sample file distributed with Galaxy that enables tools
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     3 # to use a directory of indexed data files. This one is for Bowtie2 and Tophat2.
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     4 # See the wiki: http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup
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     5 # First create these data files and save them in your own data directory structure.
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     6 # Then, create a bowtie_indices.loc file to use those indexes with tools.
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     7 # Copy this file, save it with the same name (minus the .sample), 
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     8 # follow the format examples, and store the result in this directory.
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     9 # The file should include an one line entry for each index set.
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    10 # The path points to the "basename" for the set, not a specific file.
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    11 # It has four text columns seperated by TABS.
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    12 #
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    13 # <unique_build_id>	<dbkey>	<display_name>	<file_base_path>
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    14 #
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    15 # So, for example, if you had hg18 indexes stored in:
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    16 #
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    17 #    /depot/data2/galaxy/hg19/bowtie2/
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    18 #
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    19 # containing hg19 genome and hg19.*.bt2 files, such as:
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    20 #    -rw-rw-r-- 1 james   james   914M Feb 10 18:56 hg19canon.fa
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    21 #    -rw-rw-r-- 1 james   james   914M Feb 10 18:56 hg19canon.1.bt2
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    22 #    -rw-rw-r-- 1 james   james   683M Feb 10 18:56 hg19canon.2.bt2
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    23 #    -rw-rw-r-- 1 james   james   3.3K Feb 10 16:54 hg19canon.3.bt2
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    24 #    -rw-rw-r-- 1 james   james   683M Feb 10 16:54 hg19canon.4.bt2
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    25 #    -rw-rw-r-- 1 james   james   914M Feb 10 20:45 hg19canon.rev.1.bt2
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    26 #    -rw-rw-r-- 1 james   james   683M Feb 10 20:45 hg19canon.rev.2.bt2
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    27 #
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    28 # then the bowtie2_indices.loc entry could look like this:
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    29 #
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    30 #hg19	hg19	Human (hg19)	/depot/data2/galaxy/hg19/bowtie2/hg19canon
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    31 #
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    32 #More examples:
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    33 #
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    34 #mm10	mm10	Mouse (mm10)	/depot/data2/galaxy/mm10/bowtie2/mm10
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    35 #dm3	dm3		D. melanogaster (dm3)	/depot/data2/galaxy/mm10/bowtie2/dm3
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    36 #
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    37 #
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