changeset 0:028232b595b4 draft default tip

Imported from capsule None
author devteam
date Mon, 19 May 2014 10:59:50 -0400
parents
children
files blat_coverage_report.py blat_coverage_report.xml test-data/blat_coverage_report_test1.out test-data/blat_coverage_report_test1.txt
diffstat 4 files changed, 2154 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/blat_coverage_report.py	Mon May 19 10:59:50 2014 -0400
@@ -0,0 +1,107 @@
+#!/usr/bin/env python
+
+import os, sys
+
+assert sys.version_info[:2] >= ( 2, 4 )
+
+def stop_err( msg ):
+    sys.stderr.write( "%s\n" % msg )
+    sys.exit()
+
+def reverse_complement(s):
+    complement_dna = {"A":"T", "T":"A", "C":"G", "G":"C", "a":"t", "t":"a", "c":"g", "g":"c", "N":"N", "n":"n" , ".":"."}
+    reversed_s = []
+    for i in s:
+        reversed_s.append(complement_dna[i])
+    reversed_s.reverse()
+    return "".join(reversed_s)
+
+def __main__():
+    nuc_index = {'a':0,'t':1,'c':2,'g':3}
+    diff_hash = {}    # key = (chrom, index)
+    infile = sys.argv[1]
+    outfile = sys.argv[2]
+    invalid_lines = 0
+    invalid_chars = 0
+    data_id = ''
+    data_seq = ''
+
+    for i, line in enumerate( open( infile ) ):
+        line = line.rstrip( '\r\n' )
+        if not line or line.startswith( '#' ):
+            continue
+        fields = line.split()
+        if len(fields) != 23:    # standard number of pslx columns
+            invalid_lines += 1
+            continue
+        if not fields[0].isdigit():
+            invalid_lines += 1
+            continue
+        read_id = fields[9]
+        chrom = fields[13]
+        try:
+            block_count = int(fields[17])
+        except:
+            invalid_lines += 1
+            continue
+        block_size = fields[18].split(',')
+        read_start = fields[19].split(',')
+        chrom_start = fields[20].split(',')
+        read_seq = fields[21].split(',')
+        chrom_seq = fields[22].split(',')
+
+        for j in range(block_count):
+            try:
+                this_block_size = int(block_size[j])
+                this_read_start = int(read_start[j])
+                this_chrom_start = int(chrom_start[j])
+            except:
+                invalid_lines += 1
+                break
+            this_read_seq = read_seq[j]
+            this_chrom_seq = chrom_seq[j]
+            
+            if not this_read_seq.isalpha():
+                continue
+            if not this_chrom_seq.isalpha():
+                continue
+            
+            # brut force to check coverage                
+            for k in range(this_block_size):
+                cur_index = this_chrom_start+k
+                sub_a = this_read_seq[k:(k+1)].lower()
+                sub_b = this_chrom_seq[k:(k+1)].lower()
+                if not diff_hash.has_key((chrom, cur_index)):
+                    try:
+                        diff_hash[(chrom, cur_index)] = [0,0,0,0,sub_b.upper()]    # a, t, c, g, ref. nuc.
+                    except Exception, e:
+                        stop_err( str( e ) )
+                if sub_a in ['a','t','c','g']:
+                    diff_hash[(chrom, cur_index)][nuc_index[(sub_a)]] += 1
+                else:
+                    invalid_chars += 1
+                        
+    outputfh = open(outfile, 'w')
+    outputfh.write( "##title\tlocation\tref.\tcov.\tA\tT\tC\tG\n" )
+    keys = diff_hash.keys()
+    keys.sort()
+    for i in keys:
+        (chrom, location) = i
+        sum = diff_hash[ (i) ][ 0 ] + diff_hash[ ( i ) ][ 1 ] + diff_hash[ ( i ) ][ 2 ] + diff_hash[ ( i ) ][ 3 ]    # did not include N's
+        if sum == 0:
+            continue
+        ratio_A = diff_hash[ ( i ) ][ 0 ] * 100.0 / sum
+        ratio_T = diff_hash[ ( i ) ][ 1 ] * 100.0 / sum
+        ratio_C = diff_hash[ ( i ) ][ 2 ] * 100.0 / sum
+        ratio_G = diff_hash[ ( i ) ][ 3 ] * 100.0 / sum
+        (title_head, title_tail) = os.path.split(chrom)
+        result = "%s\t%s\t%s\t%d\tA(%0.0f)\tT(%0.0f)\tC(%0.0f)\tG(%0.0f)\n" % ( title_tail, location, diff_hash[(i)][4], sum, ratio_A, ratio_T, ratio_C, ratio_G ) 
+        outputfh.write(result)
+    outputfh.close()
+
+    if invalid_lines:
+        print 'Skipped %d invalid lines. ' % ( invalid_lines )
+    if invalid_chars:
+        print 'Skipped %d invalid characters in the alignment. ' % (invalid_chars)
+        
+if __name__ == '__main__': __main__()
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/blat_coverage_report.xml	Mon May 19 10:59:50 2014 -0400
@@ -0,0 +1,75 @@
+<tool id="generate_coverage_report" name="Polymorphism of the Reads" version="1.0.0">
+	<description>the percentage of reads supporting each nucleotide at each location</description>
+	<command interpreter="python">blat_coverage_report.py $input1 $output1</command>
+	<inputs>	
+		<param name="input1" type="data" format="tabular" label="Alignment result"/>
+	</inputs>
+	<outputs>
+		<data name="output1" format="tabular"/>
+	</outputs> 
+	<tests>
+		<test>
+		<param name="input1" value="blat_coverage_report_test1.txt" ftype="tabular" />
+		<output name="output1" file="blat_coverage_report_test1.out" />
+		</test>
+	</tests>
+	<help>
+
+.. class:: warningmark
+
+**IMPORTANT**. Only works for BLAT **standard** or **pslx** output formats (hint: to output pslx format, add **-out=pslx** in the command).
+
+-----
+	
+**What it does**
+ 
+ The tool will generate a table of 6 columns as following:
+ 
+- 1st column: chromosome id.
+
+- 2nd column: chromosome location.
+
+- 3rd column: the nucleotide from reference genome at the chromosome location (2nd column).
+
+- 4th column: total coverage of the reads (number of reads that were mapped to the chromosome location).
+
+- 5th column: percentage of reads that support nucleotide **A** at this location.
+
+- 6th column: percentage of reads that support nucleotide **T** at this location.
+
+- 7th column: percentage of reads that support nucleotide **C** at this location.
+
+- 8th column: percentage of reads that support nucleotide **G** at this location.
+ 
+ 
+-----
+
+**Example**
+
+- The BLAT pslx results look like the following (tab separated with sequence at the end)::
+
+	30	0	0	0	0	0	0	0	+	seq0	30	0	30	chr	4639675	4549207	4549237	1	30,	0,	4549207,	cggacagcgccgccaccaacaaagccacca,	cggacagcgccgccaccaacaaagccacca,
+	30	0	0	0	0	0	0	0	+	seq1	30	0	30	chr	4639675	614777	614807	1	30,	0,	614777,		aaaacaccggatgctccggcgctggcagat,	aaaacaccggatgctccggcgctggcagat,
+	28	1	0	0	0	0	0	0	+	seq2	30	0	29	chr	4639675	3289283	3289312	1	29,	0,	3289283,	tttgcttttagtacaccggattcagaacc,	tttgctttcagtacaccggattcagaacc,
+	30	0	0	0	0	0	0	0	+	seq4	30	0	30	chr	4639675	2665584	2665614	1	30,	0,	2665584,	cacgctacgtgcgcccccgcccagaaggcg,	cacgctacgtgcgcccccgcccagaaggcg,
+
+	The 14th column is the chromosome id, and the 16th and 17th columns shows the reads were mapped to chromosome start and end locations.	
+
+- The report showed overall coverage of reads on each chromosome location (partial result)::
+ 
+   +-------+----------+------+------+--------+------+--------+------+
+   | title | location | ref. | cov. |   A    |  T   |   C    |  G   |
+   +-------+----------+------+------+--------+------+--------+------+	
+   |   chr |   614777 |	 A   |  1   | A(100) | T(0) |	C(0) | G(0) |
+   |   chr |   614778 |  A   |	1   | A(100) | T(0) |   C(0) | G(0) |
+   |   chr |   614779 |  A   |  1   | A(100) | T(0) |   C(0) | G(0) |
+   +-------+----------+------+------+--------+------+--------+------+	
+	
+-----
+
+**Reference**
+ 
+ **BLAT**: Kent, W James, BLAT--the BLAST-like alignment tool. (2002) Genome Research:12(4) 656-664.
+	
+	</help>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/blat_coverage_report_test1.out	Mon May 19 10:59:50 2014 -0400
@@ -0,0 +1,1908 @@
+##title	location	ref.	cov.	A	T	C	G
+chr	159366	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159367	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159368	C	1	A(0)	T(0)	C(100)	G(0)
+chr	159369	A	1	A(100)	T(0)	C(0)	G(0)
+chr	159370	A	1	A(100)	T(0)	C(0)	G(0)
+chr	159371	C	1	A(0)	T(0)	C(100)	G(0)
+chr	159372	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159373	C	1	A(0)	T(0)	C(100)	G(0)
+chr	159374	A	1	A(100)	T(0)	C(0)	G(0)
+chr	159375	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159376	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159377	C	1	A(0)	T(0)	C(100)	G(0)
+chr	159378	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159379	C	1	A(0)	T(0)	C(100)	G(0)
+chr	159380	A	1	A(100)	T(0)	C(0)	G(0)
+chr	159381	T	1	A(0)	T(100)	C(0)	G(0)
+chr	159382	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159383	A	1	A(100)	T(0)	C(0)	G(0)
+chr	159384	T	1	A(0)	T(100)	C(0)	G(0)
+chr	159385	T	1	A(0)	T(100)	C(0)	G(0)
+chr	159386	C	1	A(0)	T(0)	C(100)	G(0)
+chr	159387	T	1	A(0)	T(100)	C(0)	G(0)
+chr	159388	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159389	C	1	A(0)	T(0)	C(100)	G(0)
+chr	159390	T	1	A(0)	T(100)	C(0)	G(0)
+chr	159391	T	1	A(0)	T(100)	C(0)	G(0)
+chr	159392	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159393	G	1	A(0)	T(0)	C(0)	G(100)
+chr	159394	A	1	A(100)	T(0)	C(0)	G(0)
+chr	159395	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256715	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256716	G	1	A(0)	T(0)	C(0)	G(100)
+chr	256717	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256718	G	1	A(0)	T(0)	C(0)	G(100)
+chr	256719	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256720	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256721	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256722	T	1	A(0)	T(100)	C(0)	G(0)
+chr	256723	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256724	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256725	T	1	A(0)	T(100)	C(0)	G(0)
+chr	256726	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256727	G	1	A(0)	T(0)	C(0)	G(100)
+chr	256728	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256729	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256730	G	1	A(0)	T(0)	C(0)	G(100)
+chr	256731	T	1	A(0)	T(100)	C(0)	G(0)
+chr	256732	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256733	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256734	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256735	G	1	A(0)	T(0)	C(0)	G(100)
+chr	256736	G	1	A(0)	T(0)	C(0)	G(100)
+chr	256737	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256738	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256739	C	1	A(0)	T(0)	C(100)	G(0)
+chr	256740	G	1	A(0)	T(0)	C(0)	G(100)
+chr	256741	T	1	A(0)	T(100)	C(0)	G(0)
+chr	256742	A	1	A(100)	T(0)	C(0)	G(0)
+chr	256743	T	1	A(0)	T(100)	C(0)	G(0)
+chr	256744	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421739	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421740	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421741	A	1	A(100)	T(0)	C(0)	G(0)
+chr	421742	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421743	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421744	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421745	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421746	A	1	A(100)	T(0)	C(0)	G(0)
+chr	421747	A	1	A(100)	T(0)	C(0)	G(0)
+chr	421748	A	1	A(100)	T(0)	C(0)	G(0)
+chr	421749	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421750	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421751	C	1	A(0)	T(0)	C(100)	G(0)
+chr	421752	A	1	A(100)	T(0)	C(0)	G(0)
+chr	421753	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421754	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421755	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421756	C	1	A(0)	T(0)	C(100)	G(0)
+chr	421757	A	1	A(100)	T(0)	C(0)	G(0)
+chr	421758	C	1	A(0)	T(0)	C(100)	G(0)
+chr	421759	C	1	A(0)	T(0)	C(100)	G(0)
+chr	421760	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421761	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421762	C	1	A(0)	T(0)	C(100)	G(0)
+chr	421763	C	1	A(0)	T(0)	C(100)	G(0)
+chr	421764	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421765	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421766	T	1	A(0)	T(100)	C(0)	G(0)
+chr	421767	G	1	A(0)	T(0)	C(0)	G(100)
+chr	421768	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609915	A	1	A(100)	T(0)	C(0)	G(0)
+chr	609916	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609917	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609918	A	1	A(100)	T(0)	C(0)	G(0)
+chr	609919	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609920	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609921	G	1	A(0)	T(0)	C(0)	G(100)
+chr	609922	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609923	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609924	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609925	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609926	G	1	A(0)	T(0)	C(0)	G(100)
+chr	609927	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609928	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609929	A	1	A(100)	T(0)	C(0)	G(0)
+chr	609930	A	1	A(100)	T(0)	C(0)	G(0)
+chr	609931	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609932	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609933	G	1	A(0)	T(0)	C(0)	G(100)
+chr	609934	G	1	A(0)	T(0)	C(0)	G(100)
+chr	609935	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609936	A	1	A(100)	T(0)	C(0)	G(0)
+chr	609937	C	1	A(0)	T(0)	C(100)	G(0)
+chr	609938	G	1	A(0)	T(0)	C(0)	G(100)
+chr	609939	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609940	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609941	T	1	A(0)	T(100)	C(0)	G(0)
+chr	609942	A	1	A(100)	T(0)	C(0)	G(0)
+chr	609943	A	1	A(100)	T(0)	C(0)	G(0)
+chr	609944	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614777	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614778	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614779	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614780	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614781	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614782	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614783	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614784	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614785	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614786	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614787	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614788	T	1	A(0)	T(100)	C(0)	G(0)
+chr	614789	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614790	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614791	T	1	A(0)	T(100)	C(0)	G(0)
+chr	614792	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614793	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614794	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614795	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614796	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614797	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614798	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614799	T	1	A(0)	T(100)	C(0)	G(0)
+chr	614800	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614801	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614802	C	1	A(0)	T(0)	C(100)	G(0)
+chr	614803	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614804	G	1	A(0)	T(0)	C(0)	G(100)
+chr	614805	A	1	A(100)	T(0)	C(0)	G(0)
+chr	614806	T	1	A(0)	T(100)	C(0)	G(0)
+chr	699412	G	1	A(0)	T(0)	C(0)	G(100)
+chr	699413	A	1	A(100)	T(0)	C(0)	G(0)
+chr	699414	A	1	A(100)	T(0)	C(0)	G(0)
+chr	699415	G	1	A(0)	T(0)	C(0)	G(100)
+chr	699416	G	1	A(0)	T(0)	C(0)	G(100)
+chr	699417	A	1	A(100)	T(0)	C(0)	G(0)
+chr	699418	T	1	A(0)	T(100)	C(0)	G(0)
+chr	699419	A	1	A(100)	T(0)	C(0)	G(0)
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+chr	4535183	C	1	A(0)	T(0)	C(100)	G(0)
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+chr	4590702	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590703	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590704	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590705	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590706	T	1	A(0)	T(100)	C(0)	G(0)
+chr	4590707	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590708	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590709	T	1	A(0)	T(100)	C(0)	G(0)
+chr	4590710	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590711	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590712	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590713	T	1	A(0)	T(100)	C(0)	G(0)
+chr	4590714	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590715	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590716	C	1	A(0)	T(0)	C(100)	G(0)
+chr	4590717	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590718	T	1	A(0)	T(100)	C(0)	G(0)
+chr	4590719	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590720	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590721	T	1	A(0)	T(100)	C(0)	G(0)
+chr	4590722	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590723	C	1	A(0)	T(0)	C(100)	G(0)
+chr	4590724	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590725	G	1	A(0)	T(0)	C(0)	G(100)
+chr	4590726	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590727	C	1	A(0)	T(0)	C(100)	G(0)
+chr	4590728	T	1	A(0)	T(100)	C(0)	G(0)
+chr	4590729	A	1	A(100)	T(0)	C(0)	G(0)
+chr	4590730	T	1	A(0)	T(100)	C(0)	G(0)
+chr	4590731	G	1	A(0)	T(0)	C(0)	G(100)
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/blat_coverage_report_test1.txt	Mon May 19 10:59:50 2014 -0400
@@ -0,0 +1,64 @@
+30	0	0	0	0	0	0	0	+	0_0.666667	30	0	30	chr	4639675	4549207	4549237	1	30,	0,	4549207,	cggacagcgccgccaccaacaaagccacca,	cggacagcgccgccaccaacaaagccacca,
+30	0	0	0	0	0	0	0	+	1_0.600000	30	0	30	chr	4639675	614777	614807	1	30,	0,	614777,	aaaacaccggatgctccggcgctggcagat,	aaaacaccggatgctccggcgctggcagat,
+28	1	0	0	0	0	0	0	+	2_0.400000	30	0	29	chr	4639675	3289283	3289312	1	29,	0,	3289283,	tttgcttttagtacaccggattcagaacc,	tttgctttcagtacaccggattcagaacc,
+30	0	0	0	0	0	0	0	+	4_0.766667	30	0	30	chr	4639675	2665584	2665614	1	30,	0,	2665584,	cacgctacgtgcgcccccgcccagaaggcg,	cacgctacgtgcgcccccgcccagaaggcg,
+20	0	0	0	0	0	0	0	+	5_0.533333	30	4	24	chr	4639675	3375780	3375800	1	20,	4,	3375780,	aaaacccgccgaagcgggtt,	aaaacccgccgaagcgggtt,
+30	0	0	0	0	0	0	0	+	6_0.533333	30	0	30	chr	4639675	965481	965511	1	30,	0,	965481,	taagccgttactggcagcaagtgcaggcaa,	taagccgttactggcagcaagtgcaggcaa,
+30	0	0	0	0	0	0	0	+	7_0.400000	30	0	30	chr	4639675	1781311	1781341	1	30,	0,	1781311,	tgaatttaccgttatctatcttgcctgcct,	tgaatttaccgttatctatcttgcctgcct,
+29	1	0	0	0	0	0	0	+	9_0.400000	30	0	30	chr	4639675	4400977	4401007	1	30,	0,	4400977,	gcgttttgctaaacttctgccggaatataa,	gcgctttgctaaacttctgccggaatataa,
+30	0	0	0	0	0	0	0	+	11_0.533333	30	0	30	chr	4639675	3923551	3923581	1	30,	0,	3923551,	caaaagcagagtctgttgacccatacgcgc,	caaaagcagagtctgttgacccatacgcgc,
+28	2	0	0	0	0	0	0	+	12_0.700000	30	0	30	chr	4639675	3304453	3304483	1	30,	0,	3304453,	gcggacgatcttcacggtcgccacgcggac,	gcggacgatcttcacggtcaccacgcgggc,
+29	1	0	0	0	0	0	0	+	13_0.533333	30	0	30	chr	4639675	4555547	4555577	1	30,	0,	4555547,	ttcttgttggatggcatactccggcagcca,	ttcttgttggatggcgtactccggcagcca,
+30	0	0	0	0	0	0	0	+	14_0.666667	30	0	30	chr	4639675	3811672	3811702	1	30,	0,	3811672,	accccgatatcgtcgcaggcgttgccgcac,	accccgatatcgtcgcaggcgttgccgcac,
+29	1	0	0	0	0	0	0	+	16_0.566667	30	0	30	chr	4639675	1916143	1916173	1	30,	0,	1916143,	atgtcctgatcgagcggcgttttaccgacc,	atgtcctgatcgagcggcgttttaccgatc,
+30	0	0	0	0	0	0	0	+	18_0.566667	30	0	30	chr	4639675	1860631	1860661	1	30,	0,	1860631,	gtggtctcaagcccaaaggaagagtgaggc,	gtggtctcaagcccaaaggaagagtgaggc,
+30	0	0	0	0	0	0	0	+	19_0.566667	30	0	30	chr	4639675	1018466	1018496	1	30,	0,	1018466,	gttgtaagcgtcagaaccgatgcggtcggt,	gttgtaagcgtcagaaccgatgcggtcggt,
+30	0	0	0	0	0	0	0	+	22_0.566667	30	0	30	chr	4639675	2006716	2006746	1	30,	0,	2006716,	cggtattcctcagttctcgctgcatgcctg,	cggtattcctcagttctcgctgcatgcctg,
+30	0	0	0	0	0	0	0	-	23_0.600000	30	0	30	chr	4639675	4516584	4516614	1	30,	0,	4516584,	cgctactgaaggcgtggtgcgtaacggcaa,	cgctactgaaggcgtggtgcgtaacggcaa,
+30	0	0	0	0	0	0	0	-	24_0.733333	30	0	30	chr	4639675	1535123	1535153	1	30,	0,	1535123,	gaacagcagcagcgacgtggtgcgcccgcg,	gaacagcagcagcgacgtggtgcgcccgcg,
+30	0	0	0	0	0	0	0	+	27_0.600000	30	0	30	chr	4639675	3442475	3442505	1	30,	0,	3442475,	ttaccgaggccagaaccgataccacgaccc,	ttaccgaggccagaaccgataccacgaccc,
+29	1	0	0	0	0	0	0	+	29_0.700000	30	0	30	chr	4639675	2807120	2807150	1	30,	0,	2807120,	ctcgtccggcgggcggttttgccgacaagg,	ctcgtccggcgggcggttttgccgataagg,
+30	0	0	0	0	0	0	0	+	34_0.533333	30	0	30	chr	4639675	421739	421769	1	30,	0,	421739,	tgatgttaaatgcatggcacctgccggtgc,	tgatgttaaatgcatggcacctgccggtgc,
+30	0	0	0	0	0	0	0	-	35_0.466667	30	0	30	chr	4639675	1334399	1334429	1	30,	0,	1334399,	ggttaacctcgaatatctcggcaaagcagt,	ggttaacctcgaatatctcggcaaagcagt,
+30	0	0	0	0	0	0	0	+	36_0.433333	30	0	30	chr	4639675	2906502	2906532	1	30,	0,	2906502,	tgttggcaacatggcgagcgtaatcaatta,	tgttggcaacatggcgagcgtaatcaatta,
+30	0	0	0	0	0	0	0	+	38_0.533333	30	0	30	chr	4639675	3438288	3438318	1	30,	0,	3438288,	gcagcaggatcggatcgaactctggtttct,	gcagcaggatcggatcgaactctggtttct,
+29	1	0	0	0	0	0	0	+	39_0.400000	30	0	30	chr	4639675	2893756	2893786	1	30,	0,	2893756,	taatgcggcattctcctgatttattgtcac,	tattgcggcattctcctgatttattgtcac,
+29	0	0	0	0	0	0	0	+	41_0.566667	30	1	30	chr	4639675	708504	708533	1	29,	1,	708504,	gctgacggtcagcagggatacttcctgca,	gctgacggtcagcagggatacttcctgca,
+30	0	0	0	0	0	0	0	+	43_0.466667	30	0	30	chr	4639675	3483475	3483505	1	30,	0,	3483475,	gctttttccagcatcaacgccactgaacaa,	gctttttccagcatcaacgccactgaacaa,
+30	0	0	0	0	0	0	0	+	44_0.433333	30	0	30	chr	4639675	4590702	4590732	1	30,	0,	4590702,	aaagtggtagataacgtggtgcagactatg,	aaagtggtagataacgtggtgcagactatg,
+30	0	0	0	0	0	0	0	+	49_0.433333	30	0	30	chr	4639675	3341305	3341335	1	30,	0,	3341305,	cgttatacggaacaacatttaactccagcg,	cgttatacggaacaacatttaactccagcg,
+28	2	0	0	0	0	0	0	+	50_0.466667	30	0	30	chr	4639675	2777224	2777254	1	30,	0,	2777224,	ttgctgttgccatcgcttttcaggacatac,	ttgctgttgccgtcacttttcaggacatac,
+30	0	0	0	0	0	0	0	+	53_0.533333	30	0	30	chr	4639675	1768655	1768685	1	30,	0,	1768655,	ccaggcactccgccatatttttgccatgac,	ccaggcactccgccatatttttgccatgac,
+30	0	0	0	0	0	0	0	+	54_0.400000	30	0	30	chr	4639675	2359543	2359573	1	30,	0,	2359543,	gtattcttcattgatttgtaagcgggtacc,	gtattcttcattgatttgtaagcgggtacc,
+30	0	0	0	0	0	0	0	+	57_0.566667	30	0	30	chr	4639675	256715	256745	1	30,	0,	256715,	cgcgaaatcctcgacgtccaggcacgtatt,	cgcgaaatcctcgacgtccaggcacgtatt,
+30	0	0	0	0	0	0	0	+	58_0.300000	30	0	30	chr	4639675	1108478	1108508	1	30,	0,	1108478,	gttatccattaaaatagatcggatcgatat,	gttatccattaaaatagatcggatcgatat,
+30	0	0	0	0	0	0	0	+	60_0.533333	30	0	30	chr	4639675	1130738	1130768	1	30,	0,	1130738,	aatctggcgaatgctgatagcgtgaccggt,	aatctggcgaatgctgatagcgtgaccggt,
+30	0	0	0	0	0	0	0	-	61_0.366667	30	0	30	chr	4639675	1578065	1578095	1	30,	0,	1578065,	aaaaaacggctgacaaagctttcaaactga,	aaaaaacggctgacaaagctttcaaactga,
+30	0	0	0	0	0	0	0	+	63_0.566667	30	0	30	chr	4639675	3422447	3422477	1	30,	0,	3422447,	tcacgctcgcagtcaagctggcttatgcca,	tcacgctcgcagtcaagctggcttatgcca,
+30	0	0	0	0	0	0	0	+	63_0.566667	30	0	30	chr	4639675	2724848	2724878	1	30,	0,	2724848,	tcacgctcgcagtcaagctggcttatgcca,	tcacgctcgcagtcaagctggcttatgcca,
+30	0	0	0	0	0	0	0	-	63_0.566667	30	0	30	chr	4639675	4210393	4210423	1	30,	0,	4210393,	tggcataagccagcttgactgcgagcgtga,	tggcataagccagcttgactgcgagcgtga,
+30	0	0	0	0	0	0	0	-	63_0.566667	30	0	30	chr	4639675	3944054	3944084	1	30,	0,	3944054,	tggcataagccagcttgactgcgagcgtga,	tggcataagccagcttgactgcgagcgtga,
+29	1	0	0	0	0	0	0	+	64_0.500000	30	0	30	chr	4639675	4553565	4553595	1	30,	0,	4553565,	gagatgacggttgcagagtcatgcgtttga,	gagatgacggttgcagagtcatacgtttga,
+30	0	0	0	0	0	0	0	+	65_0.533333	30	0	30	chr	4639675	3154209	3154239	1	30,	0,	3154209,	tctggatcacgcgcaaacactggctatcgt,	tctggatcacgcgcaaacactggctatcgt,
+30	0	0	0	0	0	0	0	+	66_0.466667	30	0	30	chr	4639675	3330950	3330980	1	30,	0,	3330950,	tctgctttagcaaacagagtgtggtcacga,	tctgctttagcaaacagagtgtggtcacga,
+30	0	0	0	0	0	0	0	+	68_0.566667	30	0	30	chr	4639675	699412	699442	1	30,	0,	699412,	gaaggatagttggtcagcaacaccagcggc,	gaaggatagttggtcagcaacaccagcggc,
+30	0	0	0	0	0	0	0	+	69_0.733333	30	0	30	chr	4639675	3218070	3218100	1	30,	0,	3218070,	ggcgcgctgtcggccacggcgaaatcgacc,	ggcgcgctgtcggccacggcgaaatcgacc,
+29	1	0	0	0	0	0	0	+	72_0.533333	30	0	30	chr	4639675	2811982	2812012	1	30,	0,	2811982,	aactggaagggcttgggatgacacaacagc,	aactggaagggcttgggatgacgcaacagc,
+29	1	0	0	0	0	0	0	+	73_0.500000	30	0	30	chr	4639675	2480740	2480770	1	30,	0,	2480740,	tttaagcgccaaccaggcttctttggttgc,	tttaagcgccaaccaggcttctttagttgc,
+30	0	0	0	0	0	0	0	+	74_0.433333	30	0	30	chr	4639675	1904984	1905014	1	30,	0,	1904984,	acccttctttcgccatatcaaactgatgtc,	acccttctttcgccatatcaaactgatgtc,
+30	0	0	0	0	0	0	0	+	78_0.566667	30	0	30	chr	4639675	4535164	4535194	1	30,	0,	4535164,	cggagtatccgttccccaacgacaagcatc,	cggagtatccgttccccaacgacaagcatc,
+30	0	0	0	0	0	0	0	+	79_0.500000	30	0	30	chr	4639675	3598974	3599004	1	30,	0,	3598974,	aataccgggaagagacaacggggtctcttt,	aataccgggaagagacaacggggtctcttt,
+30	0	0	0	0	0	0	0	-	80_0.500000	30	0	30	chr	4639675	1333814	1333844	1	30,	0,	1333814,	gagaatcagggcttcgcaaccctgtcatta,	gagaatcagggcttcgcaaccctgtcatta,
+30	0	0	0	0	0	0	0	+	82_0.500000	30	0	30	chr	4639675	962544	962574	1	30,	0,	962544,	actgggttgctctgaacaagaaaggcgcta,	actgggttgctctgaacaagaaaggcgcta,
+30	0	0	0	0	0	0	0	+	83_0.533333	30	0	30	chr	4639675	4543338	4543368	1	30,	0,	4543338,	cgccagggacgtatcgcgtcgatatctatt,	cgccagggacgtatcgcgtcgatatctatt,
+30	0	0	0	0	0	0	0	+	86_0.566667	30	0	30	chr	4639675	159366	159396	1	30,	0,	159366,	ggcaacgcaggcgcatgattctgcttggaa,	ggcaacgcaggcgcatgattctgcttggaa,
+29	0	0	1	0	0	0	0	+	87_0.433333	30	0	30	chr	4639675	2137315	2137345	1	30,	0,	2137315,	cgctggatgaaaancgtgaatatcacacca,	cgctggatgaaaaacgtgaatatcacacca,
+30	0	0	0	0	0	0	0	+	88_0.533333	30	0	30	chr	4639675	2263609	2263639	1	30,	0,	2263609,	cgtaccgggctgaaagtagaagagcgtttc,	cgtaccgggctgaaagtagaagagcgtttc,
+30	0	0	0	0	0	0	0	+	90_0.466667	30	0	30	chr	4639675	609915	609945	1	30,	0,	609915,	atcaccgtttcgctaaccggtacgtttaac,	atcaccgtttcgctaaccggtacgtttaac,
+29	1	0	0	0	0	0	0	+	91_0.566667	30	0	30	chr	4639675	2506977	2507007	1	30,	0,	2506977,	ttcgcccggcaagcttacccaacgcttatc,	ttcgcctggcaagcttacccaacgcttatc,
+30	0	0	0	0	0	0	0	+	92_0.533333	30	0	30	chr	4639675	4109771	4109801	1	30,	0,	4109771,	ttttccccgccgttagtagcgactgcagtt,	ttttccccgccgttagtagcgactgcagtt,
+30	0	0	0	0	0	0	0	+	93_0.400000	30	0	30	chr	4639675	2250545	2250575	1	30,	0,	2250545,	ttatttgcccgatgagtcagtttattgcag,	ttatttgcccgatgagtcagtttattgcag,
+30	0	0	0	0	0	0	0	+	94_0.466667	30	0	30	chr	4639675	1274949	1274979	1	30,	0,	1274949,	ccacggtgatatctggtgccatactgataa,	ccacggtgatatctggtgccatactgataa,
+30	0	0	0	0	0	0	0	+	95_0.466667	30	0	30	chr	4639675	3279675	3279705	1	30,	0,	3279675,	tcccccgtaaggcctttctttttctttcgt,	tcccccgtaaggcctttctttttctttcgt,
+30	0	0	0	0	0	0	0	+	97_0.566667	30	0	30	chr	4639675	2779110	2779140	1	30,	0,	2779110,	tgcgccgccggattgttgctcaacatgctt,	tgcgccgccggattgttgctcaacatgctt,
+30	0	0	0	0	0	0	0	+	98_0.366667	30	0	30	chr	4639675	3729759	3729789	1	30,	0,	3729759,	aacgcgctaaccgccaataataacaaaatt,	aacgcgctaaccgccaataataacaaaatt,