# HG changeset patch # User Michael R. Crusoe # Date 1408359725 14400 # Node ID 08a599cf71d03e4c824562e1473154d6575af1c9 # Parent be2f355d68412247c836c8c3fcffcd8e199f167b Bjoern's suggestions diff -r be2f355d6841 -r 08a599cf71d0 abundance-dist-single.xml --- a/abundance-dist-single.xml Wed Aug 06 18:32:24 2014 -0400 +++ b/abundance-dist-single.xml Mon Aug 18 07:02:05 2014 -0400 @@ -1,4 +1,4 @@ - @@ -35,14 +35,14 @@ + help="(--savetable)" /> + help="Count k-mers past 255 (--no-bigcount)" /> @@ -53,12 +53,7 @@ - - - - - + diff -r be2f355d6841 -r 08a599cf71d0 abundance-dist.xml --- a/abundance-dist.xml Wed Aug 06 18:32:24 2014 -0400 +++ b/abundance-dist.xml Mon Aug 18 07:02:05 2014 -0400 @@ -31,12 +31,7 @@ - - - - - + diff -r be2f355d6841 -r 08a599cf71d0 count-median.xml --- a/count-median.xml Wed Aug 06 18:32:24 2014 -0400 +++ b/count-median.xml Mon Aug 18 07:02:05 2014 -0400 @@ -4,9 +4,10 @@ force_history_refresh="true"> - Count the median/avg k-mer abundance for each sequence in the input file, - based on the k-mer counts in the given k-mer counting table. Can be used to - estimate expression levels (mRNAseq) or coverage (genomic/metagenomic). + Count the median/avg k-mer abundance for each sequence in the + input file, based on the k-mer counts in the given k-mer + counting table. Can be used to estimate expression levels + (mRNAseq) or coverage (genomic/metagenomic). count-median.py @@ -14,13 +15,10 @@ -## The command is a Cheetah template which allows some Python based syntax. -## Lines starting hash hash are comments. Galaxy will turn newlines into spaces -mkdir output; cd output; @BINARY@ - $input_counting_table_filename - $input_sequence_filename - $output_summary_filename +$input_counting_table_filename +$input_sequence_filename +$output_summary_filename @@ -28,22 +26,22 @@ - + - - - - - + - - + + - - + + diff -r be2f355d6841 -r 08a599cf71d0 do-partition.xml --- a/do-partition.xml Wed Aug 06 18:32:24 2014 -0400 +++ b/do-partition.xml Mon Aug 18 07:02:05 2014 -0400 @@ -30,17 +30,17 @@ type="integer" value="20" label="ksize" - help="k-mer size to use" /> + help="k-mer size to use (--ksize/-k)" /> + help="number of tables to use (--n_tables/-N)" /> + help="lower bound on the tablesize to use (--min-tablesize/-x)" /> - - - - - + - - + - + diff -r be2f355d6841 -r 08a599cf71d0 filter-abund.xml --- a/filter-abund.xml Wed Aug 06 18:32:24 2014 -0400 +++ b/filter-abund.xml Mon Aug 18 07:02:05 2014 -0400 @@ -35,27 +35,24 @@ truevalue="--variable-coverage" falsevalue="" label="Variable coverage" - help="Only trim when a sequence has high enough coverage (median abundance > 20)" /> + help="Only trim when a sequence has high enough coverage; median abundance > 20 (--variable_coverage)" /> + help="Trim at k-mers below this abundance. (--cutoff)" /> - - - - - + + - + @@ -65,8 +62,10 @@ - - + + diff -r be2f355d6841 -r 08a599cf71d0 filter-below-abund.xml --- a/filter-below-abund.xml Wed Aug 06 18:32:24 2014 -0400 +++ b/filter-below-abund.xml Mon Aug 18 07:02:05 2014 -0400 @@ -2,7 +2,8 @@ name="Filter below abundance cutoff of 50" version="1.1-1" force_history_refresh="true"> - + + Trims fastq/fasta sequences at k-mers with abundance below 50 based on a provided k-mer counting table. @@ -29,11 +30,7 @@ - - - - + + + + diff -r be2f355d6841 -r 08a599cf71d0 normalize-by-median.xml --- a/normalize-by-median.xml Wed Aug 06 18:32:24 2014 -0400 +++ b/normalize-by-median.xml Mon Aug 18 07:02:05 2014 -0400 @@ -73,11 +73,7 @@ - - - - +