comparison lipory.xml @ 0:f678e282b320 draft default tip

"planemo upload"
author cpt_testbed
date Fri, 06 May 2022 07:07:23 +0000
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-1:000000000000 0:f678e282b320
1 <?xml version="1.0"?>
2 <tool id="edu.tamu.cpt.fasta.lipory" name="Identify Lipoboxes" version="19.1.0.0">
3 <description> in protein sequences</description>
4 <macros>
5 <import>macros.xml</import>
6 <import>cpt-macros.xml</import>
7 </macros>
8 <expand macro="requirements">
9 <requirement type="package" version="2019.06.08">regex</requirement>
10 </expand>
11 <command interpreter="python" detect_errors="aggressive"><![CDATA[lipory.py
12 $positional_1
13 $positional_2
14
15 --lipobox_mindist $lipobox_mindist
16 --lipobox_maxdist $lipobox_maxdist
17
18 > $default]]></command>
19 <inputs>
20 <param label="Naive orf calls" name="positional_1" type="data" format="gff3"/>
21 <param label="Genome" name="positional_2" type="data" format="fasta"/>
22
23 <param label="Minimum distance in codons to start of lipobox" name="lipobox_mindist" type="integer" value="10"/>
24 <param label="Maximum distance in codons to start of lipobox" name="lipobox_maxdist" type="integer" value="40"/>
25 </inputs>
26 <outputs>
27 <data format="gff3" name="default" label="Lipoboxes from ${on_string}"/>
28 </outputs>
29 <tests>
30 <test>
31 <param name="positional_1" value="T7_LiporyIn.gff3" />
32 <param name="positional_2" value="T7_LiporyIn.fasta" />
33 <param name="lipobox_mindist" value="10" />
34 <param name="lipobox_maxdist" value="60" />
35 <output name="default" value="T7_LiporyOut.gff3" />
36 </test>
37 </tests>
38 <help><![CDATA[
39 **What it does**
40
41 Identifies possible LipoBoxes from an input GFF3 and FASTA.
42
43 **How it works**
44
45 Searches in the first 10-40 amino acids of an input protein sequence using regular expressions
46 for a 4-amino acid motif based on the consensus sequences described in (**Babu** et al. 2006. *J
47 Bacteriol.* 188(8):2761-2773 and **Kongari** *et al.* 2018 *BMC Bioinformatics*. 19:326). The
48 amino acids allowed here are relaxed to allow for the diversity of amino acids known to occur in lipoboxes.
49
50 Position 1: ILMFTV or only A
51
52 Position 2: any residue except REKD or only W
53
54 Position 3: GAS
55
56 Position 4: C
57
58 ]]></help>
59 <expand macro="citations-2020" />
60 </tool>