Mercurial > repos > cpt_testbed > functionalworkflow
comparison lipory.xml @ 0:f678e282b320 draft default tip
"planemo upload"
author | cpt_testbed |
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date | Fri, 06 May 2022 07:07:23 +0000 |
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-1:000000000000 | 0:f678e282b320 |
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1 <?xml version="1.0"?> | |
2 <tool id="edu.tamu.cpt.fasta.lipory" name="Identify Lipoboxes" version="19.1.0.0"> | |
3 <description> in protein sequences</description> | |
4 <macros> | |
5 <import>macros.xml</import> | |
6 <import>cpt-macros.xml</import> | |
7 </macros> | |
8 <expand macro="requirements"> | |
9 <requirement type="package" version="2019.06.08">regex</requirement> | |
10 </expand> | |
11 <command interpreter="python" detect_errors="aggressive"><![CDATA[lipory.py | |
12 $positional_1 | |
13 $positional_2 | |
14 | |
15 --lipobox_mindist $lipobox_mindist | |
16 --lipobox_maxdist $lipobox_maxdist | |
17 | |
18 > $default]]></command> | |
19 <inputs> | |
20 <param label="Naive orf calls" name="positional_1" type="data" format="gff3"/> | |
21 <param label="Genome" name="positional_2" type="data" format="fasta"/> | |
22 | |
23 <param label="Minimum distance in codons to start of lipobox" name="lipobox_mindist" type="integer" value="10"/> | |
24 <param label="Maximum distance in codons to start of lipobox" name="lipobox_maxdist" type="integer" value="40"/> | |
25 </inputs> | |
26 <outputs> | |
27 <data format="gff3" name="default" label="Lipoboxes from ${on_string}"/> | |
28 </outputs> | |
29 <tests> | |
30 <test> | |
31 <param name="positional_1" value="T7_LiporyIn.gff3" /> | |
32 <param name="positional_2" value="T7_LiporyIn.fasta" /> | |
33 <param name="lipobox_mindist" value="10" /> | |
34 <param name="lipobox_maxdist" value="60" /> | |
35 <output name="default" value="T7_LiporyOut.gff3" /> | |
36 </test> | |
37 </tests> | |
38 <help><![CDATA[ | |
39 **What it does** | |
40 | |
41 Identifies possible LipoBoxes from an input GFF3 and FASTA. | |
42 | |
43 **How it works** | |
44 | |
45 Searches in the first 10-40 amino acids of an input protein sequence using regular expressions | |
46 for a 4-amino acid motif based on the consensus sequences described in (**Babu** et al. 2006. *J | |
47 Bacteriol.* 188(8):2761-2773 and **Kongari** *et al.* 2018 *BMC Bioinformatics*. 19:326). The | |
48 amino acids allowed here are relaxed to allow for the diversity of amino acids known to occur in lipoboxes. | |
49 | |
50 Position 1: ILMFTV or only A | |
51 | |
52 Position 2: any residue except REKD or only W | |
53 | |
54 Position 3: GAS | |
55 | |
56 Position 4: C | |
57 | |
58 ]]></help> | |
59 <expand macro="citations-2020" /> | |
60 </tool> |