Mercurial > repos > cpt_testbed > functionalworkflow
comparison lipory.xml @ 0:f678e282b320 draft default tip
"planemo upload"
| author | cpt_testbed |
|---|---|
| date | Fri, 06 May 2022 07:07:23 +0000 |
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| -1:000000000000 | 0:f678e282b320 |
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| 1 <?xml version="1.0"?> | |
| 2 <tool id="edu.tamu.cpt.fasta.lipory" name="Identify Lipoboxes" version="19.1.0.0"> | |
| 3 <description> in protein sequences</description> | |
| 4 <macros> | |
| 5 <import>macros.xml</import> | |
| 6 <import>cpt-macros.xml</import> | |
| 7 </macros> | |
| 8 <expand macro="requirements"> | |
| 9 <requirement type="package" version="2019.06.08">regex</requirement> | |
| 10 </expand> | |
| 11 <command interpreter="python" detect_errors="aggressive"><![CDATA[lipory.py | |
| 12 $positional_1 | |
| 13 $positional_2 | |
| 14 | |
| 15 --lipobox_mindist $lipobox_mindist | |
| 16 --lipobox_maxdist $lipobox_maxdist | |
| 17 | |
| 18 > $default]]></command> | |
| 19 <inputs> | |
| 20 <param label="Naive orf calls" name="positional_1" type="data" format="gff3"/> | |
| 21 <param label="Genome" name="positional_2" type="data" format="fasta"/> | |
| 22 | |
| 23 <param label="Minimum distance in codons to start of lipobox" name="lipobox_mindist" type="integer" value="10"/> | |
| 24 <param label="Maximum distance in codons to start of lipobox" name="lipobox_maxdist" type="integer" value="40"/> | |
| 25 </inputs> | |
| 26 <outputs> | |
| 27 <data format="gff3" name="default" label="Lipoboxes from ${on_string}"/> | |
| 28 </outputs> | |
| 29 <tests> | |
| 30 <test> | |
| 31 <param name="positional_1" value="T7_LiporyIn.gff3" /> | |
| 32 <param name="positional_2" value="T7_LiporyIn.fasta" /> | |
| 33 <param name="lipobox_mindist" value="10" /> | |
| 34 <param name="lipobox_maxdist" value="60" /> | |
| 35 <output name="default" value="T7_LiporyOut.gff3" /> | |
| 36 </test> | |
| 37 </tests> | |
| 38 <help><![CDATA[ | |
| 39 **What it does** | |
| 40 | |
| 41 Identifies possible LipoBoxes from an input GFF3 and FASTA. | |
| 42 | |
| 43 **How it works** | |
| 44 | |
| 45 Searches in the first 10-40 amino acids of an input protein sequence using regular expressions | |
| 46 for a 4-amino acid motif based on the consensus sequences described in (**Babu** et al. 2006. *J | |
| 47 Bacteriol.* 188(8):2761-2773 and **Kongari** *et al.* 2018 *BMC Bioinformatics*. 19:326). The | |
| 48 amino acids allowed here are relaxed to allow for the diversity of amino acids known to occur in lipoboxes. | |
| 49 | |
| 50 Position 1: ILMFTV or only A | |
| 51 | |
| 52 Position 2: any residue except REKD or only W | |
| 53 | |
| 54 Position 3: GAS | |
| 55 | |
| 56 Position 4: C | |
| 57 | |
| 58 ]]></help> | |
| 59 <expand macro="citations-2020" /> | |
| 60 </tool> |
