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1 <tool id="cummerbund" name="cummeRbund" version="0.0.4">
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2
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3 <description>R package designed to aid and simplify the task of analyzing Cufflinks RNA-Seq output</description>
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4
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5 <command interpreter="python">
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6 cummerbund_wrapper.py
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7 --r-script ${script_file}
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8 --html-report-from-directory "${output_html}" "${output_html.files_path}"
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9 </command>
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10
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11 <inputs>
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12 <conditional name="backend_database_source">
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13 <param name="backend_database_selector" type="select" label="Will you select a backend database file from the history or do you want to build a new one using cuffdiff output?">
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14 <option value="history" selected="true">Use backend database from the history</option>
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15 <option value="cuffdiff_output">Build backend database using cuffdiff output</option>
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16 </param>
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17 <when value="cuffdiff_output">
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18 <param format="tabular" name="isoforms_fpkm_tracking" type="data" label="Transcript FPKM tracking"/>
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19 <param format="tabular" name="isoforms_exp" type="data" label="Transcript differential expression testing"/>
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20 <param format="tabular" name="genes_fpkm_tracking" type="data" label="Gene FPKM tracking"/>
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21 <param format="tabular" name="genes_exp" type="data" label="Gene differential expression testing"/>
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22 <param format="tabular" name="tss_groups_fpkm_tracking" type="data" label="TSS groups FPKM tracking"/>
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23 <param format="tabular" name="tss_groups_exp" type="data" label="TSS groups differential expression testing"/>
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24 <param format="tabular" name="cds_fpkm_tracking" type="data" label="CDS FPKM tracking"/>
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25 <param format="tabular" name="cds_exp_diff" type="data" label="CDS FPKM differential expression testing"/>
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26 <param format="tabular" name="cds_diff" type="data" label="CDS overloading diffential expression testing"/>
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27 <param format="tabular" name="promoters_diff" type="data" label="Promoters differential expression testing"/>
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28 <param format="tabular" name="splicing_diff" type="data" label="Splicing differential expression testing"/>
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29 <param name="rebuild" type="hidden" value="TRUE"/>
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30 </when>
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31 <when value="history">
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32 <param name="input_database" type="data" format="data" label="Select backend database (sqlite)"/>
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33 </when>
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34 </conditional>
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35 <repeat name="plots" title="Plots">
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36 <param name="width" type="text" value="1280" label="The width of the image"/>
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37 <param name="height" type="text" value="960" label="The height of the image"/>
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38 <conditional name="plot">
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39 <param name="type" type="select" label="Plot type">
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40 <option value="density" selected="true">Density</option>
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41 <option value="boxplot">Boxplot</option>
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42 <option value="scatter">Scatter</option>
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43 <option value="volcano">Volcano</option>
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44 <option value="heatmap">Heatmap</option>
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45 <option value="cluster">Cluster</option>
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46 <option value="expressionplot">Expression Plot</option>
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47 <option value="expressionbarplot">Expression Bar Plot</option>
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48 </param>
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49 <when value="density">
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50 <param name="log_mode" type="boolean" truevalue="T" falsevalue="F" checked="True" label="Apply log10 transformation on FPKM values?"/>
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51 </when>
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52 <when value="boxplot">
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53 <param name="log_mode" type="boolean" truevalue="T" falsevalue="F" checked="True" label="Apply log10 transformation on FPKM values?"/>
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54 </when>
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55 <when value="scatter">
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56 <param name="x" type="text" label="Sample name for x axis"/>
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57 <param name="y" type="text" label="Sample name for y axis"/>
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58 <param name="log_mode" type="boolean" truevalue="T" falsevalue="F" checked="True" label="Apply log10 transformation on FPKM values?"/>
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59 <param name="smooth" type="boolean" truevalue="T" falsevalue="F" checked="True" label="Add a smooth-fit regression line"/>
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60 <conditional name="multiple_genes">
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61 <param name="multiple_genes_selector" type="boolean" truevalue="T" falsevalue="F" checked="False" label="Limit plot to a group of genes?"/>
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62 <when value="T">
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63 <param name="features" type="select" label="Expression levels to plot?">
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64 <option value="gene" selected="true">Genes</option>
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65 <option value="isoforms">Isoforms</option>
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66 <option value="tss">TSS</option>
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67 <option value="cds">CDS</option>
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68 </param>
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69 <repeat name="genes" title="Genes">
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70 <param name="gene_id" type="text" label="Gene ID"/>
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71 </repeat>
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72 </when>
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73 <when value="F"/>
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74 </conditional>
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75 </when>
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76 <when value="volcano">
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77 <param name="x" type="text" label="First sample name for comparison"/>
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78 <param name="y" type="text" label="Second sample name for comparison"/>
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79 <conditional name="multiple_genes">
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80 <param name="multiple_genes_selector" type="boolean" truevalue="T" falsevalue="F" checked="False" label="Limit plot to a group of genes?"/>
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81 <when value="T">
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82 <param name="features" type="select" label="Expression levels to plot?">
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83 <option value="gene" selected="true">Genes</option>
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84 <option value="isoforms">Isoforms</option>
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85 <option value="tss">TSS</option>
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86 <option value="cds">CDS</option>
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87 </param>
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88 <repeat name="genes" title="Genes">
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89 <param name="gene_id" type="text" label="Gene ID"/>
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90 </repeat>
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91 </when>
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92 <when value="F"/>
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93 </conditional>
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94 </when>
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95 <when value="heatmap">
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96 <param name="features" type="select" label="Expression levels to plot?">
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97 <option value="gene" selected="true">Genes</option>
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98 <option value="isoforms">Isoforms</option>
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99 <option value="tss">TSS</option>
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100 <option value="cds">CDS</option>
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101 </param>
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102 <repeat name="genes" title="Genes">
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103 <param name="gene_id" type="text" label="Gene ID"/>
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104 </repeat>
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105 <param name="clustering" type="select" label="Cluster by">
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106 <option value="row">Row</option>
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107 <option value="column">Column</option>
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108 <option value="both" selected="true">Both</option>
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109 <option value="none">None</option>
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110 </param>
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111 <param name="labcol" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Display column labels?"/>
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112 <param name="labrow" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Display column labels?"/>
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113 <param name="log_mode" type="boolean" truevalue="T" falsevalue="F" checked="True" label="Apply log10 transformation on FPKM values?"/>
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114 <param name="border" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="False" label="Draw border around plot?"/>
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115 </when>
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116 <when value="cluster">
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117 <param name="features" type="select" label="Expression levels to plot?">
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118 <option value="gene" selected="true">Genes</option>
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119 <option value="isoforms">Isoforms</option>
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120 <option value="tss">TSS</option>
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121 <option value="cds">CDS</option>
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122 </param>
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123 <repeat name="genes" title="Genes">
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124 <param name="gene_id" type="text" label="Gene ID"/>
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125 </repeat>
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126 <param name="k" type="text" label="Number of pre-defined clusters to attempt to find."/>
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127 <param name="iter_max" type="text" value="100" label="Max iterations"/>
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128 </when>
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129 <when value="expressionplot">
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130 <param name="features" type="select" label="Expression levels to plot?">
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131 <option value="gene" selected="true">Genes</option>
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132 <option value="isoforms">Isoforms</option>
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133 <option value="tss">TSS</option>
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134 <option value="cds">CDS</option>
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135 </param>
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136 <param name="gene_id" type="text" label="Gene ID"/>
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137 <param name="log_mode" type="boolean" truevalue="T" falsevalue="F" checked="True" label="Apply log10 transformation on FPKM values?"/>
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138 <param name="draw_summary" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="False" label="Draw a 'summary' line with mean FPKM
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139 values for each condition?"/>
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140 <param name="show_error_bars" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Draw error bars?"/>
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141 </when>
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142 <when value="expressionbarplot">
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143 <param name="features" type="select" label="Expression levels to plot?">
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144 <option value="gene" selected="true">Genes</option>
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145 <option value="isoforms">Isoforms</option>
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146 <option value="tss">TSS</option>
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147 <option value="cds">CDS</option>
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148 </param>
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149 <param name="gene_id" type="text" label="Gene ID"/>
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150 <param name="log_mode" type="boolean" truevalue="T" falsevalue="F" checked="True" label="Apply log10 transformation on FPKM values?"/>
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151 <param name="show_error_bars" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Draw error bars?"/>
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152 </when>
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153 </conditional>
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154 </repeat>
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155 </inputs>
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156
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157 <outputs>
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158 <data format="data" name="output_database" label="${tool.name} on ${on_string}: Database File (sqlite)">
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159 <filter>backend_database_source['backend_database_selector'] == "cuffdiff_output"</filter>
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160 </data>
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161 <data format="html" name="output_html" label="${tool.name} on ${on_string} (HTML)"/>
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162 </outputs>
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163
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164 <requirements>
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165 <requirement type="binary">R</requirement>
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166 </requirements>
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167
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168 <!-->
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169 <tests>
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170 <test>
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171 <param name="" value=""/>
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172 <output name="" file=""/>
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173 </test>
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174 </tests>
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175 -->
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176 <configfiles>
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177 <configfile name="script_file">
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178
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179 ## Feature Selection ##
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180 get_features <- function(myGenes, f="gene") {
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181 if (f == "isoforms")
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182 return(isoforms(myGenes))
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183 else if (f == "tss")
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184 return(TSS(myGenes))
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185 else if (f == "cds")
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186 return(CDS(myGenes))
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187 else
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188 return(myGenes)
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189 }
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190
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191 ## Main Function ##
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192
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193 ## Load cummeRbund library
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194 library("cummeRbund")
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195
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196 ## Initialize cuff object
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197 cuff <- readCufflinks(dir = "",
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198 #if $backend_database_source.backend_database_selector == "cuffdiff_output":
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199 dbFile = "${output_database}",
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200 geneFPKM = "${genes_fpkm_tracking}",
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201 geneDiff = "${genes_exp}",
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202 isoformFPKM = "${isoforms_fpkm_tracking}",
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203 isoformDiff = "${isoforms_exp}",
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204 TSSFPKM = "${tss_groups_fpkm_tracking}",
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205 TSSDiff = "${tss_groups_exp}",
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206 CDSFPKM = "${cds_fpkm_tracking}",
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207 CDSExpDiff = "${cds_exp_diff}",
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208 CDSDiff = "${cds_diff}",
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209 promoterFile = "${promoters_diff}",
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210 splicingFile = "${splicing_diff}",
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211 rebuild = T)
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212 #else:
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213 dbFile = "${backend_database_source.input_database}",
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214 rebuild = F)
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215 #end if
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216
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217 #for $i, $p in enumerate($plots, start=1):
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218 #set $filename = "plot%02d-%s.png" % ($i, $p.plot['type'])
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219 png(filename = "${filename}", width = ${p.width}, height = ${p.height})
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220
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221 ## Density plot ##
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222 #if $p.plot['type'] == "density":
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223 csDensity(genes(cuff))
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224 devname = dev.off()
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225
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226 ## Boxplot ##
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227 #elif $p.plot['type'] == "boxplot":
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228 csBoxplot(genes(cuff))
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229 devname = dev.off()
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230
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231 ## Scatter ##
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232 #elif $p.plot['type'] == "scatter":
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233 #if $p.plot.multiple_genes['multiple_genes_selector']:
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234 myGeneIds <- c()
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235 #for $g in $p.plot.multiple_genes['genes']:
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236 myGeneIds <- c(myGeneIds, "$g['gene_id']")
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237 #end for
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238 myGenes <- getGenes(cuff, myGeneIds)
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239 csScatter(get_features(myGenes, "$p.plot.multiple_genes['features']"), "${p.plot.x}", "${p.plot.y}", smooth=${p.plot.smooth})
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240 #else
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241 csScatter(genes(cuff), "${p.plot.x}", "${p.plot.y}", smooth=${p.plot.smooth})
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242 #end if
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243 devname = dev.off()
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244
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245 ## Volcano ##
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246 #elif $p.plot['type'] == "volcano":
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247 #if $p.plot.multiple_genes['multiple_genes_selector']:
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248 myGeneIds <- c()
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249 #for $g in $p.plot.multiple_genes['genes']:
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250 myGeneIds <- c(myGeneIds, "$g['gene_id']")
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251 #end for
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252 myGenes <- getGenes(cuff, myGeneIds)
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253 csVolcano(get_features(myGenes, "$p.plot.multiple_genes['features']"), "${p.plot.x}", "${p.plot.y}")
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254 #else
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255 csVolcano(genes(cuff), "${p.plot.x}", "${p.plot.y}")
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256 #end if
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257 devname = dev.off()
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258
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259 ## Heatmap ##
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260 #elif $p.plot['type'] == "heatmap":
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261 myGeneIds <- c()
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262 #for $g in $p.plot.genes:
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263 myGeneIds <- c(myGeneIds, "$g['gene_id']")
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264 #end for
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265 myGenes <- getGenes(cuff, myGeneIds)
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266 csHeatmap(get_features(myGenes, "${p.plot.features}"), clustering="${p.plot.clustering}", labCol="${p.plot.labcol}", labRow="${p.plot.labrow}", border="${p.plot.border}")
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267 devname = dev.off()
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268
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269 ## Cluster ##
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270 #elif $p.plot['type'] == "cluster":
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271 myGeneIds <- c()
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272 #for $g in $p.plot.genes:
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273 myGeneIds <- c(myGeneIds, "$g['gene_id']")
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274 #end for
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275 myGenes <- getGenes(cuff, myGeneIds)
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276 csCluster(get_features(myGenes, "${p.plot.features}"), k=${p.plot.k}, iter.max="${p.plot.iter_max}")
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277 devname = dev.off()
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278
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279 ## Expression Plot ##
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280 #elif $p.plot['type'] == "expressionplot":
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281 myGeneId <- "$p.plot.gene_id"
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282 myGenes <- getGenes(cuff, myGeneId)
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283 expressionPlot(get_features(myGenes, "${p.plot.features}"), drawSummary=${p.plot.draw_summary}, iter.max="${p.plot.show_error_bars}")
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284 devname = dev.off()
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285
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286 ## Expression Bar Plot ##
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287 #elif $p.plot['type'] == "expressionbarplot":
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288 myGeneId <- "$p.plot.gene_id"
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289 myGenes <- getGenes(cuff, myGeneId)
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290 expressionBarplot(get_features(myGenes, "${p.plot.features}"), iter.max="${p.plot.show_error_bars}")
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291 devname = dev.off()
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292 #end if
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293
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294 #end for
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295 </configfile>
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296 </configfiles>
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297
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298 <help>
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299 This tool allows for persistent storage, access, exploration, and manipulation of Cufflinks high-throughput sequencing data. In addition, provides numerous plotting functions for commonly used visualizations.
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300 </help>
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301
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302 </tool>
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