diff libs/special_gene_test_assemblies.py @ 0:6895de35a263 draft

planemo upload commit 844a891e4eaf732830043204ac636907eefb011d-dirty

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/libs/special_gene_test_assemblies.py	Thu Oct 19 18:16:51 2017 -0400
@@ -0,0 +1,66 @@
+#just an possible use, we can use it to replace H**.py, treat fliC and fljB as the target genes? 
+
+from __future__ import division
+import sys
+import os
+from Bio.Blast import NCBIXML
+from Bio import SeqIO
+
+Makebltdb="/nfs/sw/apps/blast/ncbi-blast-2.6.0+/bin/makeblastdb"
+Blastnpth="/nfs/sw/apps/blast/ncbi-blast-2.6.0+/bin/blastn"
+
+def special_gene(target_fie,database,gene_list):
+  database=database.split("/")[-1]##########1/27/2015
+  os.system(Makebltdb+' -in database/'+database+' -out '+database+'_db -dbtype nucl')##########1/28/2015
+  os.system(Blastnpth+' -query '+target_file+' -db '+database+'_db -out '+database+'_vs_'+target_file+'.xml '+'-outfmt 5')##########1/28/2015
+  xml_file=database+'_vs_'+target_file+'.xml'
+  result_handle=open(xml_file)
+  blast_record=NCBIXML.parse(result_handle)
+  records=list(blast_record)
+  E_thresh=1e-10
+  for x in gene_list:
+    handle=SeqIO.parse("database/"+database,"fasta")##########1/28/2015
+    length_list=[]
+    for y in handle:
+      if x in y.description:
+        length_x=len(y.seq)
+        length_list.append(length_x)
+    aver_len=float(sum(length_list))/len(length_list)
+    hspbit=[]
+    alignmentlist=[]
+    for record in records:
+      for alignment in record.alignments:
+        if x in alignment.hit_def: #multi gene database, so...
+          print x,"got a hit, evaluating the hit quality..."
+          score=0
+          for hsp in alignment.hsps:
+            if hsp.expect<E_thresh:
+              score+=hsp.bits
+          alignment=alignment.hit_def+':'+str(score)
+          hspbit.append(score)
+          alignmentlist.append(alignment)
+    scorelist=dict(zip(alignmentlist,hspbit))
+    score=0
+    for Htype in scorelist:
+      if scorelist[Htype]>score:
+        First_Choice=Htype
+        score=scorelist[Htype]
+    if float(score)>=0.1*aver_len:
+      print "$$$",First_Choice,"got a hit, score:",score
+    else:
+      print "$$$No ",x,"exists"
+  os.system("rm "+database+"_db.*")##########1/28/2015
+  os.system("rm "+xml_file)##########1/28/2015
+
+database=sys.argv[1]
+target_file=sys.argv[2]
+gene_list=[]
+a=1
+i=3
+while a==1:
+  try:
+    gene_list.append(sys.argv[i])
+    i+=1
+  except:
+    a=0
+special_gene(target_file,database,gene_list)