Mercurial > repos > charles_s_test > seqsero2
diff README.md @ 0:6895de35a263 draft
planemo upload commit 844a891e4eaf732830043204ac636907eefb011d-dirty
author | charles_s_test |
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date | Thu, 19 Oct 2017 18:16:51 -0400 |
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children | 0d65b71ff8df |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.md Thu Oct 19 18:16:51 2017 -0400 @@ -0,0 +1,54 @@ + +# GalaxySeqSero +#This Project has been modified by the CFSAN FDA office to work in Galaxy it also has a batch functionality as well add by the Galaxy rapper code. + +All below dependencies are the same for Seqsero. + Add this project to the galaxy tools directory and then added to tool_conf.xml + +Adding custom tools to Galaxy + +https://galaxyproject.org/admin/tools/add-tool-tutorial/ + + +# SeqSero 1.1 +Salmonella serotyping from genome sequencing data + + +# Introduction +SeqSero is a pipeline for Salmonella serotype determination from raw sequencing reads or genome assemblies. A web app is available at www.denglab.info/SeqSero + +# Dependencies +SeqSero depends on: + +1. Python 2.7 and [Biopython 1.65](http://biopython.org/wiki/Download); + +2. [Burrows-Wheeler Aligner](http://sourceforge.net/projects/bio-bwa/files/); + +3. [Samtools](http://sourceforge.net/projects/samtools/files/samtools/); + +4. [NCBI BLAST](https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastDocs&DOC_TYPE=Download); + +5. [SRA Toolkit](http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?cmd=show&f=software&m=software&s=software); + +6. [isPcr](http://hgwdev.cse.ucsc.edu/~kent/exe/linux/) written by Jim Kent. + +# Executing the code + Usage: SeqSero.py + + -m <int> (input data type, '1' for interleaved paired-end reads , '2' for separated paired-end reads, '3' for single reads, '4' for genome assembly) + + -i <file> (/path/to/input/file) + + -b <string> (algorithms for bwa mapping; 'mem' for mem, 'sam' for samse/sampe; default=sam; optional) + +# Output +Upon executing the command, a directory named 'SeqSero_result_<time_you_run_SeqSero>' will be created. Your result will be stored in 'Seqsero_result.txt' in that directory + +# Citation +Zhang S, Yin Y, Jones MB, Zhang Z, Deatherage Kaiser BL, Dinsmore BA, Fitzgerald C, Fields PI, Deng X. +Salmonella serotype determination utilizing high-throughput genome sequencing data. +**J Clin Microbiol.** 2015 May;53(5):1685-92.[PMID:25762776](http://jcm.asm.org/content/early/2015/03/05/JCM.00323-15) +======= +# GalaxySeqSero +GalaxySeqSero +>>>>>>> 99716906dbfd51e33c03185441d7af761d5f74b2