# HG changeset patch # User biomonika # Date 1409684972 14400 # Node ID 38fbf97cfce21abfe934f4b6609759d3019d2ccc # Parent 1955f03f092e1c240cb1e71068e041ac33788339 new dependencies diff -r 1955f03f092e -r 38fbf97cfce2 README --- a/README Tue Sep 02 14:16:04 2014 -0400 +++ b/README Tue Sep 02 15:09:32 2014 -0400 @@ -1,19 +1,14 @@ LinkYX -The pipeline can be executed by the following command: - -./runLinkYX.sh email@email.com hostname - -Please, make sure that all paths in config_devel are set accordingly. -Otherwise, use online version available from https://perun.metacentrum.cz/perun-registrar-non/?vo=linkYX +Fully automated pipeline for detection of sex linked genes using RNA-Seq data +bitbucket.org/biomonika/linkyx -Also, make sure that the input files are copied into the folder named 'input' and follow naming conventions: -mother_1.fastx, mother_2.fastx -father_1.fastx, mother_2.fastx -daughter_1.fastx, daughter_2.fastx -son_1.fastx, son_2.fastx +Please install following dependencies from toolshed: +toolshed.g2.bx.psu.edu/repos/devteam/fastq_groomer/fastq_groomer/1.0.4 +toolshed.g2.bx.psu.edu/repos/bhaas/trinityrnaseq/trinityrnaseq/0.0.1 +toolshed.g2.bx.psu.edu/repos/devteam/bwa_wrappers/bwa_wrapper/1.2.3 +toolshed.g2.bx.psu.edu/repos/devteam/sam_to_bam/sam_to_bam/1.1.4 +toolshed.g2.bx.psu.edu/repos/iuc/samtools_sort/samtools_sort/1.0.2 +toolshed.g2.bx.psu.edu/repos/devteam/picard/rgPicardMarkDups/1.56.0 -Instead of fastx, use fastq or fasta format, depending on the type of your -data. If only single-end reads are used, do not upload the second file. - -If you have any questions, please use following email address: michalovova.monika@gmail.com \ No newline at end of file +If you have any questions, please use following email address: biomonika@psu.edu \ No newline at end of file diff -r 1955f03f092e -r 38fbf97cfce2 tool_dependencies.xml --- a/tool_dependencies.xml Tue Sep 02 14:16:04 2014 -0400 +++ b/tool_dependencies.xml Tue Sep 02 15:09:32 2014 -0400 @@ -37,4 +37,29 @@ Bundle::BioPerl + + + + + + + + + + + + + + + + + + + + + + + + + \ No newline at end of file