# HG changeset patch # User bgruening # Date 1594674757 0 # Node ID 362ba03406b447e75cb5abcb95b20976a4f0b30d # Parent c30842f419ce49700cec3ae896de83d66f44bdaf "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4c71464b5f5047e0745067c115c37a5d06867649" diff -r c30842f419ce -r 362ba03406b4 macros.xml --- a/macros.xml Sat Apr 25 15:44:35 2020 +0000 +++ b/macros.xml Mon Jul 13 21:12:37 2020 +0000 @@ -1,15 +1,15 @@ - 0.14.1.2 + 1.3.0 galaxy0 - q6 + q7 + salmon seqtk - salmon samtools vpolo - pandas - scipy + pandas + scipy @@ -358,7 +358,7 @@ #end if salmon quant - --index $index_path + --index '$index_path' #if $quant_type.input.single_or_paired.single_or_paired_opts == 'single': --libType ${quant_type.input.single_or_paired.strandedness} #if $compressed == 'GZ': diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/complete_ref_lens.bin Binary file test-data/cached_locally/prebuilt_index/complete_ref_lens.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/ctable.bin Binary file test-data/cached_locally/prebuilt_index/ctable.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/ctg_offsets.bin Binary file test-data/cached_locally/prebuilt_index/ctg_offsets.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/duplicate_clusters.tsv --- a/test-data/cached_locally/prebuilt_index/duplicate_clusters.tsv Sat Apr 25 15:44:35 2020 +0000 +++ b/test-data/cached_locally/prebuilt_index/duplicate_clusters.tsv Mon Jul 13 21:12:37 2020 +0000 @@ -1,1 +1,1 @@ -RetainedTxp DuplicateTxp +RetainedRef DuplicateRef diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/hash.bin Binary file test-data/cached_locally/prebuilt_index/hash.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/header.json --- a/test-data/cached_locally/prebuilt_index/header.json Sat Apr 25 15:44:35 2020 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,14 +0,0 @@ -{ - "value0": { - "IndexType": 1, - "IndexVersion": "q6", - "UsesKmers": true, - "KmerLen": 31, - "BigSA": false, - "PerfectHash": false, - "SeqHash": "238a71484cdcf251805629c612f7edd515eb1a23d9be2e2014b62fd5931eb4ac", - "NameHash": "2fc64c34c65a78d18fec933772e1aa454cfed52b2b48d43eb75d2933a22feb04", - "SeqHash512": "a45ea3d0c5f11c16a45b8b75f902f908996117d7486d72b3d19a8ae619b07b22f3ca0888f5ee6e9dbabf639ae51f7fc74b8eea8808435bbb2ec70b96162849aa", - "NameHash512": "6123f1c58bf2153fca204e97fa72a2d25460242f14b886e421316ff251cc57293e77c02d536122e97f46d2508c626e42a35a1798209766359904ddf49fb7bf33" - } -} \ No newline at end of file diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/indexing.log --- a/test-data/cached_locally/prebuilt_index/indexing.log Sat Apr 25 15:44:35 2020 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -[2019-09-08 20:35:10.408] [jLog] [info] building index -[2019-09-08 20:35:10.448] [jLog] [info] done building index diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/info.json --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cached_locally/prebuilt_index/info.json Mon Jul 13 21:12:37 2020 +0000 @@ -0,0 +1,22 @@ +{ + "index_version": 4, + "reference_gfa": [ + "foo" + ], + "sampling_type": "dense", + "k": 31, + "num_kmers": 18902, + "num_contigs": 23, + "seq_length": 19592, + "have_ref_seq": true, + "have_edge_vec": false, + "SeqHash": "238a71484cdcf251805629c612f7edd515eb1a23d9be2e2014b62fd5931eb4ac", + "NameHash": "2fc64c34c65a78d18fec933772e1aa454cfed52b2b48d43eb75d2933a22feb04", + "SeqHash512": "a45ea3d0c5f11c16a45b8b75f902f908996117d7486d72b3d19a8ae619b07b22f3ca0888f5ee6e9dbabf639ae51f7fc74b8eea8808435bbb2ec70b96162849aa", + "NameHash512": "6123f1c58bf2153fca204e97fa72a2d25460242f14b886e421316ff251cc57293e77c02d536122e97f46d2508c626e42a35a1798209766359904ddf49fb7bf33", + "DecoySeqHash": "e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855", + "DecoyNameHash": "e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855", + "num_decoys": 0, + "first_decoy_index": 18446744073709551615, + "keep_duplicates": false +} \ No newline at end of file diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/mphf.bin Binary file test-data/cached_locally/prebuilt_index/mphf.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/pos.bin Binary file test-data/cached_locally/prebuilt_index/pos.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/pre_indexing.log --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cached_locally/prebuilt_index/pre_indexing.log Mon Jul 13 21:12:37 2020 +0000 @@ -0,0 +1,3 @@ +[2020-07-09 14:16:39.483] [jLog] [warning] The salmon index is being built without any decoy sequences. It is recommended that decoy sequence (either computed auxiliary decoy sequence or the genome of the organism) be provided during indexing. Further details can be found at https://salmon.readthedocs.io/en/latest/salmon.html#preparing-transcriptome-indices-mapping-based-mode. +[2020-07-09 14:16:39.484] [jLog] [info] building index +[2020-07-09 14:16:39.619] [jLog] [info] done building index diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/quasi_index.log --- a/test-data/cached_locally/prebuilt_index/quasi_index.log Sat Apr 25 15:44:35 2020 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,12 +0,0 @@ -[2019-09-08 20:35:10.409] [jointLog] [info] [Step 1 of 4] : counting k-mers -[2019-09-08 20:35:10.412] [jointLog] [info] Replaced 0 non-ATCG nucleotides -[2019-09-08 20:35:10.412] [jointLog] [info] Clipped poly-A tails from 0 transcripts -[2019-09-08 20:35:10.412] [jointLog] [info] Building rank-select dictionary and saving to disk -[2019-09-08 20:35:10.412] [jointLog] [info] done -[2019-09-08 20:35:10.412] [jointLog] [info] Writing sequence data to file . . . -[2019-09-08 20:35:10.412] [jointLog] [info] done -[2019-09-08 20:35:10.412] [jointLog] [info] Building 32-bit suffix array (length of generalized text is 28,577) -[2019-09-08 20:35:10.413] [jointLog] [info] Building suffix array . . . -[2019-09-08 20:35:10.445] [jointLog] [info] khash had 18,902 keys -[2019-09-08 20:35:10.445] [jointLog] [info] saving hash to disk . . . -[2019-09-08 20:35:10.447] [jointLog] [info] done diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/rank.bin Binary file test-data/cached_locally/prebuilt_index/rank.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/refAccumLengths.bin Binary file test-data/cached_locally/prebuilt_index/refAccumLengths.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/refInfo.json --- a/test-data/cached_locally/prebuilt_index/refInfo.json Sat Apr 25 15:44:35 2020 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,5 +0,0 @@ -{ - "ReferenceFiles": [ - "test-data/transcripts.fasta" - ] -} \ No newline at end of file diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/ref_indexing.log --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cached_locally/prebuilt_index/ref_indexing.log Mon Jul 13 21:12:37 2020 +0000 @@ -0,0 +1,29 @@ +[2020-07-09 14:16:39.484] [puff::index::jointLog] [info] Running fixFasta +[2020-07-09 14:16:39.490] [puff::index::jointLog] [info] Replaced 0 non-ATCG nucleotides +[2020-07-09 14:16:39.490] [puff::index::jointLog] [info] Clipped poly-A tails from 0 transcripts +[2020-07-09 14:16:39.491] [puff::index::jointLog] [info] Filter size not provided; estimating from number of distinct k-mers +[2020-07-09 14:16:39.494] [puff::index::jointLog] [info] ntHll estimated 54811 distinct k-mers, setting filter size to 2^20 +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Starting the Pufferfish indexing by reading the GFA binary file. +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Setting the index/BinaryGfa directory foo +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Done wrapping the rank vector with a rank9sel structure. +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] contig count for validation: 23 +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Total # of Contigs : 23 +[2020-07-09 14:16:39.546] [puff::index::jointLog] [info] Total # of numerical Contigs : 23 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] Total # of contig vec entries: 36 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] bits per offset entry 6 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] Done constructing the contig vector. 24 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] # segments = 23 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] total length = 19,592 +[2020-07-09 14:16:39.547] [puff::index::jointLog] [info] Reading the reference files ... +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] positional integer width = 15 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] seqSize = 19,592 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] rankSize = 19,592 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] edgeVecSize = 0 +[2020-07-09 14:16:39.548] [puff::index::jointLog] [info] num keys = 18,902 +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] mphf size = 0.0125198 MB +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] chunk size = 9,796 +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] chunk 0 = [0, 9,796) +[2020-07-09 14:16:39.614] [puff::index::jointLog] [info] chunk 1 = [9,796, 19,562) +[2020-07-09 14:16:39.618] [puff::index::jointLog] [info] finished populating pos vector +[2020-07-09 14:16:39.618] [puff::index::jointLog] [info] writing index components +[2020-07-09 14:16:39.619] [puff::index::jointLog] [info] finished writing dense pufferfish index diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/reflengths.bin Binary file test-data/cached_locally/prebuilt_index/reflengths.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/refseq.bin Binary file test-data/cached_locally/prebuilt_index/refseq.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/rsd.bin Binary file test-data/cached_locally/prebuilt_index/rsd.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/sa.bin Binary file test-data/cached_locally/prebuilt_index/sa.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/seq.bin Binary file test-data/cached_locally/prebuilt_index/seq.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/txpInfo.bin Binary file test-data/cached_locally/prebuilt_index/txpInfo.bin has changed diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/prebuilt_index/versionInfo.json --- a/test-data/cached_locally/prebuilt_index/versionInfo.json Sat Apr 25 15:44:35 2020 +0000 +++ b/test-data/cached_locally/prebuilt_index/versionInfo.json Mon Jul 13 21:12:37 2020 +0000 @@ -1,6 +1,7 @@ { - "indexVersion": 4, + "indexVersion": 5, "hasAuxIndex": false, "auxKmerLength": 31, - "indexType": 1 + "indexType": 2, + "salmonVersion": "1.3.0" } \ No newline at end of file diff -r c30842f419ce -r 362ba03406b4 test-data/cached_locally/salmon_indexes_versioned.loc --- a/test-data/cached_locally/salmon_indexes_versioned.loc Sat Apr 25 15:44:35 2020 +0000 +++ b/test-data/cached_locally/salmon_indexes_versioned.loc Mon Jul 13 21:12:37 2020 +0000 @@ -30,4 +30,4 @@ #dm3 dm3 D. melanogaster (dm3) /depot/data2/galaxy/salmon_indexes/dm3/version_1 q5 # # -hg19_transcript_subset hg19 Human (Homo sapiens): hg19 transcript test subset ${__HERE__}/prebuilt_index q6 +hg19_transcript_subset hg19 Human (Homo sapiens): hg19 transcript test subset ${__HERE__}/prebuilt_index q7