# HG changeset patch
# User bgruening
# Date 1761734719 0
# Node ID 244d10cb5fbe154b0b08937afa5c1ab4cbad41c5
# Parent  d7346aaf11f4a216f8cc067dd56bddb46422f1a4
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 1971e35a365a91ae36c990b374a552a46196e888

diff -r d7346aaf11f4 -r 244d10cb5fbe macros.xml
--- a/macros.xml	Fri Jun 23 17:01:09 2023 +0000
+++ b/macros.xml	Wed Oct 29 10:45:19 2025 +0000
@@ -1,8 +1,13 @@
 <macros>
     <token name="@TOOL_VERSION@">1.10.1</token>
-    <token name="@VERSION_SUFFIX@">0</token>
+    <token name="@VERSION_SUFFIX@">3</token>
     <token name="@IDX_VERSION@">q7</token>
-    <token name="@PROFILE_VERSION@">20.01</token>
+    <token name="@PROFILE_VERSION@">24.1</token>
+    <xml name="xrefs">
+        <xrefs>
+            <xref type="bio.tools">salmon</xref>
+        </xrefs>
+    </xml>
     <xml name="requirements">
         <requirements>
             <requirement type="package" version="@TOOL_VERSION@">salmon</requirement>
@@ -14,8 +19,11 @@
             <requirement type="package" version="1.9.3">scipy</requirement>
         </requirements>
     </xml>
+    <xml name="version_command">
+        <version_command><![CDATA[salmon --no-version-check --version | cut -d" " -f2]]></version_command>
+    </xml>
     <xml name="orient">
-        <param name="orientation" type="select" label="Relative orientation of reads within a pair">
+        <param argument="--libType" name="orientation" type="select" label="Relative orientation of reads within a pair">
             <option value="M">Mates are oriented in the same direction (M = matching)</option>
             <option value="O">Mates are oriented away from each other (O = outward)</option>
             <option value="I" selected="True">Mates are oriented toward each other (I = inward)</option>
@@ -23,7 +31,7 @@
     </xml>
     <xml name="stranded">
         <conditional name="libtype" >
-            <param name="strandedness" type="select" label="Specify the strandedness of the reads" help="--libtype">
+            <param argument="--libType" name="strandedness" type="select" label="Specify the strandedness of the reads" help="--libtype">
                 <option value="A" selected="true">Infer automatically (A)</option>
                 <option value="U">Not stranded (U)</option>
                 <option value="SF">read comes from the forward strand (SF)</option>
@@ -63,7 +71,7 @@
                 <option value="history" selected="True">Use one from the history</option>
             </param>
             <when value="indexed">
-                <param name="index" type="select" label="Select a reference transcriptome" help="If your transcriptome of interest is not listed, contact your Galaxy admin">
+                <param argument="--index" type="select" label="Select a reference transcriptome" help="If your transcriptome of interest is not listed, contact your Galaxy admin">
                     <options from_data_table="salmon_indexes_versioned">
                         <filter type="sort_by" column="2"/>
                         <filter type="static_value" column="4" value="@IDX_VERSION@" />
@@ -73,14 +81,11 @@
             </when>  <!-- build-in -->
             <when value="history">
                 <section name="s_index" title="Salmon index" expanded="true">
-                    <param name="fasta" type="data" format="fasta" label="Transcripts FASTA file"/>
-                    <param name="genome" type="data" format="fasta" optional="true" label="Reference genome" 
+                    <param argument="--transcripts" name="fasta" type="data" format="fasta" label="Transcripts FASTA file"/>
+                    <param argument="--genome" type="data" format="fasta" optional="true" label="Reference genome" 
                         help="The reference genome is required for generating a decoy-away index. The decoy sequences are regions of the target genome 
                             that are sequence similar to annotated transcripts. These are the regions of the genome most likely to cause mismapping." />
-                    <param name="kmer" type="integer" value="31" label="Kmer length"/>
-                    <param name="phash" type="boolean" label="Perfect Hash" truevalue="--perfectHash" falsevalue="" checked="false"
-                        help="Build the index using a perfect hash rather than a dense hash.  This will require less memory (especially during quantification), 
-                            but will take longer to construct"/>
+                    <param argument="--kmerLen" name="kmer" type="integer" value="31" label="Kmer length"/>
                 </section>
             </when>
         </conditional>
@@ -90,75 +95,62 @@
             <conditional name="single_or_paired">
                 <param name="single_or_paired_opts" type="select" label="Is this library mate-paired?">
                     <option value="single">Single-end</option>
-                    <option value="paired">Paired-end</option>
                     <option value="paired_collection">Paired-end Dataset Collection</option>
-                    <option value="paired_interleaved">Paired-end data from single interleaved dataset</option>
                 </param>
                 <when value="single">
-                    <param name="input_singles" type="data" format="fastq,fasta,fastq.gz,fastq.bz2" label="FASTQ/FASTA file" help="FASTQ file." />
+                    <param argument="--unmatedReads" name="input_singles" type="data" format="fasta,fastqsanger,fastqsanger.gz,fastqsanger.bz2" label="FASTQ/FASTA file" help="FASTQ file." />
                     <expand macro="stranded_se"/>
                 </when>
-                <when value="paired">
-                    <param name="input_mate1" type="data" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" label="Mate pair 1" help="FASTQ file." />
-                    <param name="input_mate2" type="data" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" label="Mate pair 2" help="FASTQ file." />
+                <when value="paired_collection">
+                    <param name="input_1" format="fasta,fastqsanger,fastqsanger.gz,fastqsanger.bz2" type="data_collection" collection_type="paired" label="FASTQ Paired Dataset" help="(--mates1,--mates2)" />
                     <expand macro="stranded_pe"/>
                 </when>
-                <when value="paired_collection">
-                    <param name="input_1" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" type="data_collection" collection_type="paired" label="FASTQ Paired Dataset" help="Must be of datatype &quot;fastqsanger&quot; or &quot;fasta&quot;" />
-                    <expand macro="stranded_pe"/>
-                </when>
-                <when value="paired_interleaved">
-                   <param name="input_1" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" type="data" label="Interleaved FASTQ file" help="Must be of datatype &quot;fastqsanger&quot; or &quot;fasta&quot;. --interleaved"/>
-                   <expand macro="stranded_pe"/>
-                </when>
             </conditional>
         </section>
-        <param argument="--type" type="select" label="Type of index" help="When using quasi, orphaned reads will be considered when performing lightweight-alignment.">
-            <option value="quasi" selected="True">quasi</option>
-        </param>
         <!--Salmon quant type select: reads-based or alignment-based-->
-        <param name="discardOrphansQuasi" type="boolean" truevalue="--discardOrphansQuasi" falsevalue="" checked="False"
+        <param argument="--discardOrphansQuasi" type="boolean" truevalue="--discardOrphansQuasi" falsevalue="" checked="False"
         label="Discard orphan quasi"
         help="Discard orphan mappings in quasi-mapping mode. If this flag is passed then only paired mappings will be considered toward quantification estimates.  The default behavior is to consider orphan mappings if no valid paired mappings exist."/>
         <conditional name="validmap">
-            <param name="validateMappings" type="boolean" truevalue="--validateMappings" falsevalue="" checked="False"
-            label="Validate mappings"
-            help="Validate mappings using alignment-based verifcation. If this flag is passed, quasi-mappings will be validated to ensure that they could give rise to a reasonable alignment before they are further used for quantification."/>
+            <param name="validateMappings" type="select" label="Validate mappings" help="Validate mappings using alignment-based verifcation. If this flag is passed, quasi-mappings will be validated to ensure that they could give rise to a reasonable alignment before they are further used for quantification.">
+                <option value="--validateMappings">True</option>
+                <option value="" selected="true">False</option>
+            </param>
             <when value="--validateMappings">
-                <param name="minScoreFraction" type="float" value="0.65" min="0.0" max="0.99"
+                <param argument="--minScoreFraction" type="float" value="0.65" min="0.0" max="0.99"
                 label="Min Score Fraction"
                 help="The fraction of the optimal possible alignment score that a mapping must achieve in order to be considered valid. Should be in (0,1]."/>
-                <param name="maxMMPExtension" type="integer" optional="True"
+                <param argument="--maxMMPExtension" type="integer" optional="True"
                 label="Sets the maximum allowable MMP extension when collecting suffix array intervals to be used in chaining. This prevents MMPs from becoming too long, and potentially masking intervals that would uncover other good quasi-mappings for the read. This heuristic mimics the idea of the maximum mappable safe prefix (MMSP) in selective alignment.  Setting a smaller value will potentially allow for more sensitive, but slower, mapping."/>
-                <param name="matchScore" argument="--ma" type="integer" value="2"
+                <param argument="--ma" name="matchScore" type="integer" value="2"
                 label="Match Score"
                 help="The value given to a match between read and reference nucleotides in an alignment."/>
-                <param name="mismatchPenalty" argument="--mp" type="integer" value="4"
+                <param argument="--mp" name="mismatchPenalty" type="integer" value="4"
                 label="Mismatch Penalty"
                 help="The value given to a mis-match between read and reference nucleotides in an alignment. This will be cast to a negative value."/>
-                <param name="gapOpenPenalty" argument="--go" type="integer" value="5"
+                <param argument="--go" name="gapOpenPenalty" type="integer" value="5"
                 label="Gap Open Penalty"
                 help="The value given to a gap opening in an alignment."/>
-                <param name="gapExtensionPenalty" argument="--ge" type="integer" value="3"
+                <param argument="--ge" name="gapExtensionPenalty" type="integer" value="3"
                 label="Gap Extension Penalty"
                 help="The value given to a gap extension in an alignment."/>
-                <param name="mimicBT2" type="boolean" truevalue="--mimicBT2" falsevalue="" checked="False"
+                <param argument="--mimicBT2" type="boolean" truevalue="--mimicBT2" falsevalue="" checked="False"
                 label="Mimic Bowtie 2"
                 help=" Set flags to mimic parameters similar to Bowtie2 with --no-discordant and --no-mixed flags. This increases disallows dovetailing reads, and discards orphans. Note, this does not impose the very strict parameters assumed by RSEM+Bowtie2, like gapless alignments. For that behavior, use the --mimiStrictBT2 flag "/>
-                <param name="mimicStrictBT2" type="boolean" truevalue="--mimicStrictBT2" falsevalue="" checked="False"
+                <param argument="--mimicStrictBT2" type="boolean" truevalue="--mimicStrictBT2" falsevalue="" checked="False"
                 label="Mimic Strict Bowtie 2"
                 help="Set flags to mimic the very strict parameters used by RSEM+Bowtie2. This increases --minScoreFraction to 0.8, disallows dovetailing reads, discards orphans, and disallows gaps in alignments."/>
-                <param name="hardFilter" type="boolean" truevalue="--hardFilter" falsevalue="" checked="False"
+                <param argument="--hardFilter" type="boolean" truevalue="--hardFilter" falsevalue="" checked="False"
                 label="Hard Filter"
                 help="Instead of weighting mappings by their alignment score, this flag will discard any mappings with sub-optimal alignment score. The default option of soft-filtering (i.e. weighting mappings by their alignment score) usually yields slightly more accurate abundance estimates but this flag may be desirable if you want more accurate 'naive' equivalence classes, rather than range factorized equivalence classes."/>
             </when>
             <when value=""/>
         </conditional>
-        <param name="consensusSlack" type="integer" optional="True" value="0"
+        <param argument="--consensusSlack" type="integer" optional="True" value="0"
         label="Consensus Slack"
         help="The amount of slack allowed in the quasi-mapping consensus mechanism.  Normally, a transcript must cover all hits to be considered for mapping. If this is set to a value, X, greater than 0, then a transcript can fail to cover up to X hits before it is discounted as a mapping candidate.  The default value of this option is 1 if --validateMappings is given and 0 otherwise."/>
-        <param name="dovetail" label="Allow Dovetail" type="boolean" truevalue="--allowDovetail" falsevalue="" checked="False" help="Allow dovetail mappings"/>
-        <param name="recoverOrphans" label="Recover Orphans" type="boolean" truevalue="--recoverOrphans" falsevalue="" checked="False"
+        <param argument="--dovetail" label="Allow Dovetail" type="boolean" truevalue="--allowDovetail" falsevalue="" checked="False" help="Allow dovetail mappings"/>
+        <param argument="--recoverOrphans" label="Recover Orphans" type="boolean" truevalue="--recoverOrphans" falsevalue="" checked="False"
         help="Attempt to recover the mates of orphaned reads. This uses edlib for orphan recovery, and so introduces some computational overhead, but it can improve sensitivity."/>
         <conditional name="bam_options">
             <param argument="--writeMappings" type="select" label="Write Mappings to BAM File" help="If this option is provided, then the quasi-mapping results will be written out in SAM-compatible format.  By default, output will be directed to stdout, but an alternative file name can be provided instead">
@@ -171,152 +163,135 @@
             </when>
             <when value=""/>
         </conditional>
-       <param name="consistentHits" type="boolean" truevalue="--consistentHits" falsevalue="" checked="False"
+       <param argument="--consistentHits" type="boolean" truevalue="--consistentHits" falsevalue="" checked="False"
         label="Consistent Hits"
         help="Force hits gathered during quasi-mapping to be 'consistent' (i.e. co-linear and approximately the right distance apart)."/>
-        <param name="quasiCoverage" type="integer" optional="True" label="Quasi Coverage"
+        <param argument="--quasiCoverage" type="integer" optional="True" label="Quasi Coverage"
         help="[Experimental]: The fraction of the read that must be covered by MMPs (of length >= 31) if this read is to be considered as 'mapped'. This may help to avoid 'spurious' mappings. A value of 0 (the default) denotes no coverage threshold (a single 31-mer can yield a mapping). Since coverage by exact matching, large, MMPs is a rather strict condition, this value should likely be set to something low, if used."/>
     </xml>
     <xml name="align">
-        <param name="afile" type="data" format="qname_input_sorted.bam,qname_sorted.bam" label="Alignment file"/>
+        <param argument="--alignments" name="afile" type="data" format="qname_input_sorted.bam,qname_sorted.bam" label="Alignment file"/>
         <param argument="--ont" type="boolean" truevalue="--ont" falsevalue="" label="Is this Alignment file a Oxford Nanopore Technologies (ONT) dataset ?" help="Select this for both cDNA and direct RNA ONT datasets. Enables an alignment error model designed to work with long-read alignments and disables the length effect in the generative model when computing the conditional probability of observing a fragment given that it arises from a specific transcript. This is because in long-read sequencing, we do not expect to observe (i.e. sequence) multiple fragments from the same molecule, and thus we do not expect the transcript length to directly affect the observed fragment count directly"/>
-        <param name="transcript" type="data" format="fasta,fa" label="Transcript file"/>
+        <param argument="--targets" name="transcript" type="data" format="fasta,fasta.gz" label="Transcript file"/>
         <expand macro="stranded_se"/>
-        <param name="discardOrphans" type="boolean" truevalue="--discardOrphans" falsevalue="" checked="False"
+        <param argument="--discardOrphans" type="boolean" truevalue="--discardOrphans" falsevalue="" checked="False"
         label="Discard orphans"
         help="Discard orphan alignments in the input. If this flag is passed, then only paired alignments will be considered toward quantification estimates. The default behavior is to consider orphan alignments if no valid paired mappings exist."/>
-        <param name="noErrorModel" type="boolean" truevalue="--noErrorModel" falsevalue="" checked="False"
+        <param argument="--noErrorModel" type="boolean" truevalue="--noErrorModel" falsevalue="" checked="False"
         label="No Error Model"
         help="Turn off the alignment error model, which takes into account the the observed frequency of different types of mismatches / indels when computing the likelihood of a given alignment. Turning this off can speed up alignment-based salmon, but can harm quantification accuracy."/>
-        <param name="numErrorBins" type="integer" optional="True" value="6"
+        <param argument="--numErrorBins" type="integer" optional="True" value="6"
         label="Number of Error Bins"
         help="The number of bins into which to divideeach read when learning and applying the error model. For example, a value of 10 would mean that effectively, a separate error model is learned and applied to each 10th of the read, whilea value of 3 would mean that a separate error model is applied to the read beginning (first third), middle (second third) and end (final third)."/>
-        <param name="sampleOut" type="boolean" truevalue="--sampleOut" falsevalue="" checked="False"
+        <param argument="--sampleOut" type="boolean" truevalue="--sampleOut" falsevalue="" checked="False"
         label="Sample out"
         help="Write a 'postSample.bam' file in the output directory that will sample the input alignments according to the estimated transcript abundances. If you're going to perform downstream analysis of the alignments with tools which don't, themselves, take fragment assignment ambiguity into account, you should use this output"/>
-        <param name="sampleUnaligned" type="boolean" truevalue="--sampleUnaligned" falsevalue="" checked="False"
+        <param argument="--sampleUnaligned" type="boolean" truevalue="--sampleUnaligned" falsevalue="" checked="False"
         label="Sample unaligned"
         help="In addition to sampling the aligned reads, also write the un-aligned reads to 'postSample.bam'."/>
-        <param name="gencode" type="boolean" truevalue="--gencode" falsevalue="" checked="False"
+        <param argument="--gencode" type="boolean" truevalue="--gencode" falsevalue="" checked="False"
         label="Gencode"
         help="This flag will expect the input transcript fasta to be in GENCODE format, and will split the transcript name at the first '|' character.  These reduced names will be used in the output and when looking for these transcripts in a gene to transcript GTF."/>
     </xml>
     <xml name="quantboth">
-        <param name="geneMap" type="data" format="tabular,gff,gtf" optional="True"
+        <param argument="--geneMap" type="data" format="tabular,gff,gtf" optional="True"
         label="File containing a mapping of transcripts to genes"
         help="If this file is provided Salmon will output both quant.sf and quant.genes.sf files, where the latter contains aggregated gene-level abundance estimates. The transcript to gene mapping should be provided as either a GTF file, or a in a simple tab-delimited format where each line contains the name of a transcript and the gene to which it belongs separated by a tab."/>
-        <param name="seqBias" type="boolean" truevalue="--seqBias" falsevalue="" checked="False"
+        <param argument="--seqBias" type="boolean" truevalue="--seqBias" falsevalue="" checked="False"
         label="Perform sequence-specific bias correction"/>
-        <param name="gcBias" type="boolean" truevalue="--gcBias" falsevalue="" checked="False"
+        <param argument="--gcBias" type="boolean" truevalue="--gcBias" falsevalue="" checked="False"
         label="Perform fragment GC bias correction"/>
-        <param name="incompatPrior" type="float" optional="True" min="0.0" max="1" value="0"
+        <param argument="--incompatPrior" type="float" optional="True" min="0.0" max="1" value="0"
         help="Sets the prior probability that an alignment that disagrees with the specified library type (orientation and strandedness) results from the true fragment origin. Setting this to 0 specifies that alignments that disagree with the library type should be 'impossible', while setting it to 1 says that alignments that disagree with the library type are no less likely than those that do"/>
-        <param name="meta" type="boolean" truevalue="--meta" falsevalue="" checked="False"
+        <param argument="--meta" type="boolean" truevalue="--meta" falsevalue="" checked="False"
         label="Meta"
         help="If you're using Salmon on a metagenomic dataset, consider setting this flag to disable parts of the abundance estimation model that make less sense for metagenomic data."/>
         <!--Salmon quant advanced options: available to both kinds of quant-->
         <section name="adv" title="Additional Options">
-            <param name="skipQuant" type="boolean" truevalue="--skipQuant" falsevalue="" checked="False"
+            <param argument="--skipQuant" type="boolean" truevalue="--skipQuant" falsevalue="" checked="False"
             label="skipQuant" help="Skip performing the actual transcript quantification (including any Gibbs sampling or bootstrapping)."/>
-            <param name="dumpEq" type="boolean" truevalue="--dumpEq" falsevalue="" checked="False"
+            <param argument="--dumpEq" type="boolean" truevalue="--dumpEq" falsevalue="" checked="False"
             label="Dump equivalence class counts"
             help="Dump the equivalence class counts that were computed during quasi-mapping."/>
-            <param name="dumpEqWeights" type="boolean" truevalue="--dumpEqWeights" falsevalue="" checked="False"
+            <param argument="--dumpEqWeights" type="boolean" truevalue="--dumpEqWeights" falsevalue="" checked="False"
             label="Dump equivalence class counts including rich weights"
             help="Includes 'rich' equivlance class weights in the output when equivalence class information is being dumped to file."/>
-            <param name="minAssignedFrags" type="integer" optional="True" label="Minimum assigned fragments"
+            <param argument="--minAssignedFrags" type="integer" optional="True" label="Minimum assigned fragments"
             help="The minimum number of fragments that must be assigned to the transcriptome for quantification to proceed."/>
-            <param name="biasSpeedSamp" type="integer" value="5" optional="True"
+            <param argument="--biasSpeedSamp" type="integer" value="5" optional="True"
             label="The value at which the fragment length PMF is down-sampled when evaluating GC fragment bias." help="Larger values speed up effective length correction, but may decrease the fidelity of bias modeling results."/>
-            <param name="fldMax" type="integer" value="1000" optional="True"
+            <param argument="--fldMax" type="integer" value="1000" optional="True"
             label="The maximum fragment length to consider when building the empirical distribution."/>
-            <param name="fldMean" type="integer" value="250" optional="True"
+            <param argument="--fldMean" type="integer" value="250" optional="True"
             label="The mean used in the fragment length distribution prior"
             help="If single end reads are being used for quantification, or there are an insufficient number of uniquely mapping reads when performing paired-end quantification to estimate the empirical fragment length distribution, then use this value to calculate effective lengths."/>
-            <param name="fldSD" type="integer" value="25" optional="True"
+            <param argument="--fldSD" type="integer" value="25" optional="True"
             label="Standard deviation"
             help="The standard deviation used in the fragment length distribution prior."/>
-            <param name="forgettingFactor" type="float" value="0.65000000000000002" optional="True"
+            <param argument="--forgettingFactor" type="float" value="0.65000000000000002" optional="True"
             label="The forgetting factor used in the online learning schedule."
             help=" A smaller value results in quicker learning, but higher variance and may be unstable. A larger value results in slower learning but may be more stable.  Value should be in the interval (0.5, 1.0]." />
-            <param name="initUniform" type="boolean" truevalue="--initUniform" falsevalue="" checked="False"
+            <param argument="--initUniform" type="boolean" truevalue="--initUniform" falsevalue="" checked="False"
             label="Initialization with uniform parameters"
             help="initialize the offline inference with uniform parameters, rather than seeding with online parameters." />
-            <param name="maxReadOcc" type="integer" value="100" optional="True"
+            <param argument="--maxReadOcc" type="integer" value="100" optional="True"
             label="Maximal read mapping occurence"
             help="Reads mapping to more than this many places won't be considered."/>
-            <param name="noLengthCorrection" type="boolean" truevalue="--noLengthCorrection" falsevalue=""
+            <param argument="--noLengthCorrection" type="boolean" truevalue="--noLengthCorrection" falsevalue=""
             label="No length correction"
             help="[experimental] : Entirely disables length correction when estimating the abundance of transcripts.  This option can be used with protocols where one expects that fragments derive from their underlying targets without regard to that target's length (e.g. QuantSeq)"/>
-            <param name="noEffectiveLengthCorrection" type="boolean" truevalue="--noEffectiveLengthCorrection" falsevalue="" checked="False"
+            <param argument="--noEffectiveLengthCorrection" type="boolean" truevalue="--noEffectiveLengthCorrection" falsevalue="" checked="False"
             label="Disable effective length correction"
             help="Disables effective length correction when computing the probability that a fragment was generated from a transcript. If this flag is passed in, the fragment length distribution is not taken into account when computing this probability."/>
-            <param name="noFragLengthDist" type="boolean" truevalue="--noFragLengthDist" falsevalue="" checked="False"
+            <param argument="--noFragLengthDist" type="boolean" truevalue="--noFragLengthDist" falsevalue="" checked="False"
             label="Ignore fragment length distribution"
             help="[experimental] : Don't consider concordance with the learned fragment length distribution when trying to determine the probability that a fragment has originated from a specified location.  Normally, Fragments with unlikely lengths will be assigned a smaller relative probability than those with more likely lengths. When this flag is passed in, the observed fragment length has no effect on that fragment's a priori probability." />
-            <param name="noBiasLengthThreshold" type="boolean" truevalue="--noBiasLengthThreshold" falsevalue="" checked="False"
+            <param argument="--noBiasLengthThreshold" type="boolean" truevalue="--noBiasLengthThreshold" falsevalue="" checked="False"
             label="[experimental] : If this option is enabled, then no (lower) threshold will be set on how short bias correction can make effecctive lengths."
             help="This can increase the precision of bias correction, but harm robustness. The default correction applies a threshold." />
-            <param name="numBiasSamples" type="integer" value="2000000" optional="True"
+            <param argument="--numBiasSamples" type="integer" value="2000000" optional="True"
             label="Number of fragment mappings to use when learning the sequence-specific bias model."/>
-            <param name="numAuxModelSamples" type="integer" value="5000000" optional="True"
+            <param argument="--numAuxModelSamples" type="integer" value="5000000" optional="True"
             label="The first numAuxModelSamples are used to train the auxiliary model parameters."
             help="(e.g. fragment length distribution, bias, etc.). After ther first numAuxModelSamples observations the auxiliary model parameters will be assumed to have converged and will be fixed." />
-            <param name="numPreAuxModelSamples" type="integer" value="5000" optional="True"
+            <param argument="--numPreAuxModelSamples" type="integer" value="5000" optional="True"
             label="The first numPreAuxModelSamples will have their assignment likelihoods and contributions to the transcript abundances computed without applying any auxiliary models."
             help=" The purpose of ignoring the auxiliary models for the first numPreAuxModelSamples observations is to avoid applying these models before thier parameters have been learned sufficiently well." />
-            <param name="useEM" type="boolean" truevalue="--useEM" falsevalue="" checked="False"
+            <param argument="--useEM" type="boolean" truevalue="--useEM" falsevalue="" checked="False"
             label="Use the traditional EM algorithm for optimization in the batch passes."
             help=""/>
-            <param name="rangeFactorizationBins" type="integer" value="0" label="Range of factorization bins"
+            <param argument="--rangeFactorizationBins" type="integer" value="0" label="Range of factorization bins"
             help="Factorizes the likelihood used in quantification by adopting a new notion of equivalence classes based on the conditional probabilities with which fragments are generated from different transcripts. This is a more fine-grained factorization than the normal rich equivalence classes. The default value (0) corresponds to the standard rich equivalence classes, and larger values imply a more fine-grained factorization. If range factorization is enabled, a common value to select for this parameter is 4."/>
-            <param name="numGibbsSamples" type="integer" value="0" optional="True"
+            <param argument="--numGibbsSamples" type="integer" value="0" optional="True"
             label="Number of Gibbs sampling rounds to perform."
             help="" />
-            <param name="noGammaDraw" type="boolean" truevalue="--noGammaDraw" falsevalue="" checked="False"
+            <param argument="--noGammaDraw" type="boolean" truevalue="--noGammaDraw" falsevalue="" checked="False"
             label="No gamma draw"
             help="This switch will disable drawing transcript fractions from a Gamma distribution during Gibbs sampling. In this case the sampler does not account for shot-noise, but only assignment ambiguity"/>
-            <param name="numBootstraps" type="integer" value="0" optional="True"
+            <param argument="--numBootstraps" type="integer" value="0" optional="True"
             label="Number of bootstrap samples to generate. Note: This is mutually exclusive with Gibbs sampling."
             help="" />
-            <param name="bootstrapReproject" type="boolean" truevalue="--bootstrapReproject" falsevalue="" checked="False"
+            <param argument="--bootstrapReproject" type="boolean" truevalue="--bootstrapReproject" falsevalue="" checked="False"
             label="Bootstrap reproject"
             help="This switch will learn the parameter distribution from the bootstrapped counts for each sample, but will reproject those parameters onto the original equivalence class counts."/>
-            <param name="thinningFactor" type="integer" value="16" optional="true" label="Thinning factor"
+            <param argument="--thinningFactor" type="integer" value="16" optional="true" label="Thinning factor"
             help="Number of steps to discard for every sample kept from the Gibbs chain. The larger this number, the less chance that subsequent samples are auto-correlated, but the slower sampling becomes."/>
-            <param name="perTranscriptPrior" type="boolean" truevalue="--perTranscriptPrior" falsevalue="" checked="False"
+            <param argument="--perTranscriptPrior" type="boolean" truevalue="--perTranscriptPrior" falsevalue="" checked="False"
             label="The prior will be interpreted as a transcript-level prior."
             help="either the default or the argument provided via --vbPrior" />
-            <param name="sigDigits" type="integer" value="3"
+            <param argument="--sigDigits" type="integer" value="3"
             label="Significant Digits"
             help="The number of significant digits to write when outputting the EffectiveLength and NumReads columns."/>
-            <param name="vbPrior" type="float" value="1.0000000000000001e-05" optional="True"
+            <param argument="--vbPrior" type="float" value="1.0000000000000001e-05" optional="True"
             label="The prior that will be used in the VBEM algorithm."
             help="This is interpreted as a per-nucleotide prior, unless the --perTranscriptPrior flag is also given, in which case this is used as a transcript-level prior." />
-            <param name="writeOrphanLinks" type="boolean" truevalue="--writeOrphanLinks" falsevalue="" checked="False" label="Write orphan links"
+            <param argument="--writeOrphanLinks" type="boolean" truevalue="--writeOrphanLinks" falsevalue="" checked="False" label="Write orphan links"
             help="Write the transcripts that are linked by orphaned reads."/>
-            <param name="writeUnmappedNames" type="boolean" truevalue="--writeUnmappedNames" falsevalue="" checked="False"
+            <param argument="--writeUnmappedNames" type="boolean" truevalue="--writeUnmappedNames" falsevalue="" checked="False"
             label="Write the names of un-mapped reads to the file unmapped_names.txt."
             help=""/>
         </section>
     </xml>
-    <xml name="quantmerge">
-        <repeat name="quantfiles" title="Quant file and names">
-            <param name="quants" type="data" format="tsv,tabular"
-            label="Salmon quant tabular output files"/>
-            <param name="names" type="text" label="One-word sample names" optional="True"/>
-        </repeat>
-        <param name="column" type="select" label="Columns"
-        help="Name of columns that will be merged in the output file">
-            <option value="len" selected="True">Length</option>
-            <option value="elen">Effective Length</option>
-            <option value="tpm">Transcripts per Million</option>
-            <option value="numreads">NumReads</option>
-        </param>
-        <param name="genes" type="boolean" truevalue="--genes" falsevalue="" checked="False"
-        label="Genes" help="Use gene quantifications instead of transcript"/>
-        <param name="missing" type="integer" label="Missing" optional="True" help="The value of missing values"/>
-    </xml>
     <xml name="citations">
         <citations>
             <citation type="doi">10.1186/s13059-019-1670-y</citation>
@@ -327,26 +302,26 @@
     <token name="@indexing@"><![CDATA[
         mkdir ./index &&
         mkdir ./output &&
-        #if $quant_type.refTranscriptSource.TranscriptSource != "indexed":
-            #if $quant_type.refTranscriptSource.s_index.genome
-                cat '${quant_type.refTranscriptSource.s_index.genome}' | grep "^>" | cut -d " " -f 1 > 'decoys.txt' &&
+        #if $TRANSCRIPTSOURCE_CONDITIONAL.TranscriptSource != "indexed":
+            #if $TRANSCRIPTSOURCE_CONDITIONAL.s_index.genome
+                cat '${TRANSCRIPTSOURCE_CONDITIONAL.s_index.genome}' | grep "^>" | cut -d " " -f 1 > 'decoys.txt' &&
                 sed -i.bak -e 's/>//g' 'decoys.txt' &&
-                cat '${quant_type.refTranscriptSource.s_index.fasta}' '${quant_type.refTranscriptSource.s_index.genome}' > 'input_index.fasta' &&
+                cat '${TRANSCRIPTSOURCE_CONDITIONAL.s_index.fasta}' '${TRANSCRIPTSOURCE_CONDITIONAL.s_index.genome}' > 'input_index.fasta' &&
             #else
-                ln -s '${quant_type.refTranscriptSource.s_index.fasta}' 'input_index.fasta' &&
+                ln -s '${TRANSCRIPTSOURCE_CONDITIONAL.s_index.fasta}' 'input_index.fasta' &&
             #end if
-            salmon index -i ./index
-                --kmerLen '${quant_type.refTranscriptSource.s_index.kmer}'
+            salmon --no-version-check index -i ./index
+                --kmerLen '${TRANSCRIPTSOURCE_CONDITIONAL.s_index.kmer}'
                 --gencode
-                --threads "\${GALAXY_SLOTS:-4}"
+                --threads "\$SLOTS"
                 --transcripts 'input_index.fasta'
-                #if $quant_type.refTranscriptSource.s_index.genome
+                #if $TRANSCRIPTSOURCE_CONDITIONAL.s_index.genome
                     --decoy 'decoys.txt'
                 #end if
             &&
             #set $index_path = './index'
         #else
-            #set $index_path = $quant_type.refTranscriptSource.index.fields.path
+            #set $index_path = $TRANSCRIPTSOURCE_CONDITIONAL.index.fields.path
         #end if
         ]]>
     </token>
@@ -356,58 +331,33 @@
             #if $quant_type.input.single_or_paired.input_singles.ext == 'fasta':
                 #set $ext = 'fasta'
             #else:
-                #if $quant_type.input.single_or_paired.input_singles.is_of_type("fastq.gz", "fastqsanger.gz"):
+                #if $quant_type.input.single_or_paired.input_singles.is_of_type("fastqsanger.gz"):
                     #set compressed = 'GZ'
-                #else if $quant_type.input.single_or_paired.input_singles.is_of_type("fastq.bz2", "fastqsanger.bz2"):
+                #else if $quant_type.input.single_or_paired.input_singles.is_of_type("fastqsanger.bz2"):
                     #set compressed = 'BZ2'
                 #end if
                 #set $ext = 'fastq'
             #end if
             ln -s $quant_type.input.single_or_paired.input_singles ./single.$ext &&
-        #else if $quant_type.input.single_or_paired.single_or_paired_opts == 'paired':
-            #if $quant_type.input.single_or_paired.input_mate1.ext == 'fasta':
-                #set $ext = 'fasta'
-            #else:
-                #if $quant_type.input.single_or_paired.input_mate1.is_of_type("fastq.gz", "fastqsanger.gz"):
-                    #set compressed = 'GZ'
-                #else if $quant_type.input.single_or_paired.input_mate1.is_of_type("fastq.bz2", "fastqsanger.bz2"):
-                    #set compressed = 'BZ2'
-                #end if
-                #set $ext = 'fastq'
-            #end if
-            ln -s $quant_type.input.single_or_paired.input_mate1 ./mate1.$ext &&
-            ln -s $quant_type.input.single_or_paired.input_mate2 ./mate2.$ext &&
         #else if $quant_type.input.single_or_paired.single_or_paired_opts == 'paired_collection':
             #if $quant_type.input.single_or_paired.input_1.forward.ext == 'fasta':
                 #set $ext = 'fasta'
             #else:
-                #if $quant_type.input.single_or_paired.input_1.forward.is_of_type("fastq.gz", "fastqsanger.gz"):
+                #if $quant_type.input.single_or_paired.input_1.forward.is_of_type("fastqsanger.gz"):
                     #set compressed = 'GZ'
-                #else if $quant_type.input.single_or_paired.input_1.forward.is_of_type("fastq.bz2", "fastqsanger.bz2"):
+                #else if $quant_type.input.single_or_paired.input_1.forward.is_of_type("fastqsanger.bz2"):
                     #set compressed = 'BZ2'
                 #end if
                 #set $ext = 'fastq'
             #end if
             ln -s '${quant_type.input.single_or_paired.input_1.forward}' ./mate1.$ext &&
             ln -s '${quant_type.input.single_or_paired.input_1.reverse}' ./mate2.$ext &&
-        #else if '$quant_type.input.single_or_paired.single_or_paired_opts' == 'paired_interleaved':
-            #if $quant_type.input.single_or_paired.input_1.ext == 'fasta':
-                #set $ext = 'fasta'
-            #else:
-                #if $quant_type.input.single_or_paired.input_1.is_of_type("fastq.gz", "fastqsanger.gz"):
-                    #set compressed = 'GZ'
-                #else if $quant_type.input.single_or_paired.input_1.is_of_type("fastq.bz2", "fastqsanger.bz2"):
-                    #set compressed = 'BZ2'
-                #end if
-                #set $ext = 'fastq'
-            #end if
-            ln -s '$quant_type.input.single_or_paired.input_1' ./mate1.$ext &&
         #end if
         #if $geneMap:
             ln -s '$geneMap' ./geneMap.${geneMap.ext} &&
         #end if
 
-        salmon quant
+        salmon --no-version-check quant
             --index '$index_path'
             #if $quant_type.input.single_or_paired.single_or_paired_opts == 'single':
                 --libType ${quant_type.input.single_or_paired.libtype.strandedness}
@@ -425,28 +375,18 @@
                 #else
                     ${quant_type.input.single_or_paired.libtype.orientation}${quant_type.input.single_or_paired.libtype.strandedness}
                 #end if
-                #if $quant_type.input.single_or_paired.single_or_paired_opts == 'paired_interleaved':
-                    #if $compressed == 'BZ2':
-                        --mates1 <(bzcat < ./mate1.$ext | seqtk seq -1)
-                        --mates2 <(bzcat < ./mate1.$ext | seqtk seq -2)
-                    #else:
-                        --mates1 <(seqtk seq -1 ./mate1.$ext)
-                        --mates2 <(seqtk seq -2 ./mate1.$ext)
-                    #end if
+                #if $compressed == 'GZ':
+                    --mates1 <(zcat < ./mate1.$ext)
+                    --mates2 <(zcat < ./mate2.$ext)
+                #else if $compressed == 'BZ2':
+                    --mates1 <(bzcat < ./mate1.$ext)
+                    --mates2 <(bzcat < ./mate2.$ext)
                 #else:
-                    #if $compressed == 'GZ':
-                        --mates1 <(zcat < ./mate1.$ext)
-                        --mates2 <(zcat < ./mate2.$ext)
-                    #else if $compressed == 'BZ2':
-                        --mates1 <(bzcat < ./mate1.$ext)
-                        --mates2 <(bzcat < ./mate2.$ext)
-                    #else:
-                        --mates1 ./mate1.$ext
-                        --mates2 ./mate2.$ext
-                    #end if
+                    --mates1 ./mate1.$ext
+                    --mates2 ./mate2.$ext
                 #end if
             #end if
-            --threads "\${GALAXY_SLOTS:-4}"
+            --threads "\$SLOTS"
             ${quant_type.discardOrphansQuasi}
             ${quant_type.validmap.validateMappings}
             #if $quant_type.validmap.validateMappings:
@@ -491,12 +431,12 @@
         #if $geneMap:
             ln -s "$geneMap" ./geneMap.${geneMap.ext} &&
         #end if
-        salmon quant
-        -t '${quant_type.transcript}'
-        -l '${quant_type.libtype.strandedness}'
-        -a '${quant_type.afile}'
+        salmon --no-version-check quant
+        --targets '${quant_type.transcript}'
+        --libType '${quant_type.libtype.strandedness}'
+        --alignments '${quant_type.afile}'
         $quant_type.ont
-        --threads "\${GALAXY_SLOTS:-4}"
+        --threads "\$SLOTS"
         ${quant_type.discardOrphans}
         ${quant_type.noErrorModel}
         #if $quant_type.numErrorBins:
@@ -584,33 +524,7 @@
         -o ./output
         ]]>
     </token>
-    <token name="@qmerge@"><![CDATA[
-        #import re
 
-        #for $counter, $input_data in enumerate($quantfiles):
-            rank_of_series=$counter
-            mkdir ${counter}.quantmerge &&
-            ln -s $input_data.quants ${counter}.quantmerge/quant.sf &&
-        #end for
-        salmon quantmerge
-            --quants *.quantmerge
-            --names
-            #for $counter, $input_data in enumerate($quantfiles):
-                #if $input_data.names and re.sub(' ', '', '$input_data.names') != '':
-                    '${input_data.names}'
-                #else:
-                    #set $identifier=re.sub('[^\w\-\s]', '_', str($input_data.quants.element_identifier))
-                    "$identifier"
-               #end if
-            #end for
-            --column '${column}'
-            ${genes}
-            #if $missing:
-                --missing '${missing}'
-            #end if
-            -o qmergeout.tab
-        ]]>
-    </token>
     <token name="@salmonhelp@"><![CDATA[
         Salmon is a lightweight method for quantifying transcript abundance from RNA–seq reads, combining a dual-phase parallel inference algorithm and feature-rich bias models with an ultra-fast read mapping procedure.
 
diff -r d7346aaf11f4 -r 244d10cb5fbe salmonquant.xml
--- a/salmonquant.xml	Fri Jun 23 17:01:09 2023 +0000
+++ b/salmonquant.xml	Wed Oct 29 10:45:19 2025 +0000
@@ -3,9 +3,16 @@
     <macros>
         <import>macros.xml</import>
     </macros>
+    <expand macro="xrefs"/>
     <expand macro="requirements"/>
+    <expand macro="version_command"/>
     <command detect_errors="aggressive"><![CDATA[
+        ## salmon uses one thread to much
+        ## https://github.com/COMBINE-lab/salmon/issues/993
+        SLOTS=\$(( \${GALAXY_SLOTS:-4} > 1 ? \${GALAXY_SLOTS:-4} - 1 : 1 ));
+
         #if $quant_type.qtype == "reads":
+            #set TRANSCRIPTSOURCE_CONDITIONAL = $quant_type.refTranscriptSource
             @indexing@
             @salreads@
         #else:
@@ -60,9 +67,13 @@
                 </conditional>
                 <section name="input">
                     <conditional name="single_or_paired">
-                        <param name="single_or_paired_opts" value="paired" />
-                        <param name="input_mate1" value="fastqs/reads_1.fastq" />
-                        <param name="input_mate2" value="fastqs/reads_2.fastq" />
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
                         <conditional name="libtype">
                             <param name="strandedness" value="U"/>
                         </conditional>
@@ -100,9 +111,15 @@
                     </section>
                 </conditional>
                 <section name="input">
-                    <param name="single_or_paired.single_or_paired_opts" value="paired" />
-                    <param name="single_or_paired.input_mate1" value="fastqs/reads_1.fastq" />
-                    <param name="single_or_paired.input_mate2" value="fastqs/reads_2.fastq" />
+                    <conditional name="single_or_paired">
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
+                    </conditional>
                 </section>
                 <conditional name="bam_options">
                     <param name="writeMappings" value="--writeMappings=./output/samout.sam"/>
@@ -136,10 +153,18 @@
                     </section>
                 </conditional>
                 <section name="input">
-                    <param name="single_or_paired.single_or_paired_opts" value="paired" />
-                    <param name="single_or_paired.input_mate1" value="fastqs/reads_1.fastq.gz" />
-                    <param name="single_or_paired.input_mate2" value="fastqs/reads_2.fastq.gz" />
-                    <param name="single_or_paired.strandedness" value="U"/>
+                    <conditional name="single_or_paired">
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
+                        <conditional name="libtype">
+                            <param name="strandedness" value="U"/>
+                        </conditional>
+                    </conditional>
                 </section>
             </conditional>
             <output name="output_quant" ftype="tabular">
@@ -167,9 +192,13 @@
                     </section>
                 </conditional>
                 <section name="input">
-                    <param name="single_or_paired.single_or_paired_opts" value="single" />
-                    <param name="single_or_paired.input_mate1" value="fastqs/reads_both.fastq.bz2" />
-                    <param name="single_or_paired.strandedness" value="U"/>
+                    <conditional name="single_or_paired">
+                        <param name="single_or_paired_opts" value="single" />
+                        <param name="input_singles" value="fastqs/reads_both.fastq.bz2"/>
+                        <conditional name="libtype">
+                            <param name="strandedness" value="U"/>
+                        </conditional>
+                    </conditional>
                 </section>
             </conditional>
             <output name="output_quant" ftype="tabular">
@@ -193,9 +222,9 @@
                 <param name="qtype" value="alignment"/>
                 <param name="afile" value="salmonbam.bam"/>
                 <param name="transcript" value="transcripts.fasta"/>
-                <param name="sampleOut" value="--sampleOut"/>
-                <param name="sampleUnaligned" value="--sampleUnaligned"/>
-                <param name="noErrorModel" value="--noErrorModel"/>
+                <param name="sampleOut" value="true"/>
+                <param name="sampleUnaligned" value="true"/>
+                <param name="noErrorModel" value="true"/>
                 <param name="numErrorBins" value="5"/>
             </conditional>
             <!-- bam output uses non-deterministic sampling, so can only use compare="sim_size" -->
@@ -210,9 +239,9 @@
                 <param name="afile" value="salmonbam.bam"/>
                 <param name="ont" value="true" />
                 <param name="transcript" value="transcripts.fasta"/>
-                <param name="sampleOut" value="--sampleOut"/>
-                <param name="sampleUnaligned" value="--sampleUnaligned"/>
-                <param name="noErrorModel" value="--noErrorModel"/>
+                <param name="sampleOut" value="true"/>
+                <param name="sampleUnaligned" value="true"/>
+                <param name="noErrorModel" value="true"/>
                 <param name="numErrorBins" value="5"/>
             </conditional>
             <assert_stderr>
@@ -232,17 +261,25 @@
                     </section>
                 </conditional>
                 <section name="input">
-                    <param name="single_or_paired.single_or_paired_opts" value="paired" />
-                    <param name="single_or_paired.input_mate1" value="fastqs/reads_1.fastq" />
-                    <param name="single_or_paired.input_mate2" value="fastqs/reads_2.fastq" />
-                    <param name="single_or_paired.strandeness" value="U"/>
+                    <conditional name="single_or_paired">
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
+                        <conditional name="libtype">
+                            <param name="strandedness" value="U"/>
+                        </conditional>
+                    </conditional>
                 </section>
-                <param name="discardOrphansQuasi" value="-discardOrphansQuasi"/>
+                <param name="discardOrphansQuasi" value="true"/>
                 <conditional name="validmap">
                     <param name="validateMappings" value="--validateMappings"/>
                 </conditional>
-                <param name="dovetail" value="--allowDovetail"/>
-                <param name="recoverOrphans" value="--recoverOrphans"/>
+                <param name="dovetail" value="true"/>
+                <param name="recoverOrphans" value="true"/>
             </conditional>
             <output name="output_quant" ftype="tabular">
                 <assert_contents>
@@ -265,21 +302,29 @@
                     </section>
                 </conditional>
                 <section name="input">
-                    <param name="single_or_paired.single_or_paired_opts" value="paired" />
-                    <param name="single_or_paired.input_mate1" value="fastqs/reads_1.fastq" />
-                    <param name="single_or_paired.input_mate2" value="fastqs/reads_2.fastq" />
-                    <param name="single_or_paired.strandedness" value="U"/>
+                    <conditional name="single_or_paired">
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
+                        <conditional name="libtype">
+                            <param name="strandedness" value="U"/>
+                        </conditional>
+                    </conditional>
                 </section>
             </conditional>
-            <param name="seqBias" value="--seqBias"/>
-            <param name="gcBias" value="--gcBias"/>
+            <param name="seqBias" value="true"/>
+            <param name="gcBias" value="true"/>
             <section name="adv">
-                <param name="dumpEq" value="--dumpEq"/>
+                <param name="dumpEq" value="true"/>
                 <param name="minAssignedFrags" value="10"/>
-                <param name="noLengthCorrection"/>
-                <param name="initUniform" value="--initUniform"/>
-                <param name="useEM" value="--useEM"/>
-                <param name="noGammaDraw" value="--noGammaDraw"/>
+                <!-- can not be enabled simultaneously with seqBias <param name="noLengthCorrection" value="true"/> -->
+                <param name="initUniform" value="true"/>
+                <param name="useEM" value="true"/>
+                <param name="noGammaDraw" value="true"/>
             </section>
             <output name="output_quant" ftype="tabular">
                 <assert_contents>
@@ -298,10 +343,16 @@
                 </conditional>
                 <section name="input">
                     <conditional name="single_or_paired">
-                        <param name="single_or_paired_opts" value="paired" />
-                        <param name="input_mate1" value="fastqs/reads_1.fastq" />
-                        <param name="input_mate2" value="fastqs/reads_2.fastq" />
-                        <param name="strandedness" value="U"/>
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
+                        <conditional name="libtype">
+                            <param name="strandedness" value="U"/>
+                        </conditional>
                     </conditional>
                 </section>
             </conditional>
@@ -332,9 +383,15 @@
                     </section>
                 </conditional>
                 <section name="input">
-                    <param name="single_or_paired.single_or_paired_opts" value="paired" />
-                    <param name="single_or_paired.input_mate1" value="fastqs/reads_1.fastq" />
-                    <param name="single_or_paired.input_mate2" value="fastqs/reads_2.fastq" />
+                    <conditional name="single_or_paired">
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
+                    </conditional>
                 </section>
                 <conditional name="bam_options">
                     <param name="writeMappings" value="--writeMappings=./output/samout.sam"/>
@@ -372,9 +429,13 @@
                 </conditional>
                 <section name="input">
                     <conditional name="single_or_paired">
-                        <param name="single_or_paired_opts" value="paired" />
-                        <param name="input_mate1" value="fastqs/reads_1.fastq" />
-                        <param name="input_mate2" value="fastqs/reads_2.fastq" />
+                        <param name="single_or_paired_opts" value="paired_collection" />
+                        <param name="input_1">
+                            <collection type="paired" name="mates">
+                                <element name="forward" value="fastqs/reads_1.fastq"/>
+                                <element name="reverse" value="fastqs/reads_2.fastq"/>
+                            </collection>
+                        </param>
                         <conditional name="libtype">
                             <param name="strandedness" value="U"/>
                         </conditional>
diff -r d7346aaf11f4 -r 244d10cb5fbe test-data/full_data_structure.txt
--- a/test-data/full_data_structure.txt	Fri Jun 23 17:01:09 2023 +0000
+++ b/test-data/full_data_structure.txt	Wed Oct 29 10:45:19 2025 +0000
@@ -544,7 +544,7 @@
 8	198	199	200	201	202	203	204	205	1	1	32	1	TACGGGCTGC	1
 1	170	1	1	21	1	GAACATTTGT	1
 3	29	30	31	2	2	34	1	CGGCGTCCGG	1	1	1	AGCGGCTGGT	1
-11	5	6	7	8	9	10	11	12	13	14	16	1	1	93	1	TTGACACCAT	1
+1	16	1	1	93	1	TTGACACCAT	1
 1	146	1	1	46	1	AACACAGATA	1
 4	287	289	291	293	2	2	140	1	CCGTACGGGG	1	35	1	GGATTCAGTA	1
 3	273	274	275	1	1	86	1	CCGACTCGTT	1
diff -r d7346aaf11f4 -r 244d10cb5fbe umiout.sh
--- a/umiout.sh	Fri Jun 23 17:01:09 2023 +0000
+++ b/umiout.sh	Wed Oct 29 10:45:19 2025 +0000
@@ -1,11 +1,14 @@
 #!/usr/bin/bash
 
-mkdir fixed;
+set -e
+
+mkdir -p fixed;
 for file in ./umiout/*;
-do prefix="${file%.dot.gz}";
-prefix=${prefix/.\/umiout\//};
-gunzip $file;
-sed "s/umiout\/$prefix.dot.gz/$prefix/" umiout/$prefix.dot > fixed/$prefix.dot;
-dot -Tpdf fixed/$prefix.dot -o fixed/$prefix.pdf;
+do
+prefix="${file%.dot.gz}";
+prefix="${prefix/.\/umiout\//}";
+gunzip "$file";
+sed "s/umiout\/$prefix.dot.gz/$prefix/" "umiout/$prefix.dot" > "fixed/$prefix.dot";
+dot -Tpdf "fixed/$prefix.dot" -o "fixed/$prefix.pdf";
 done
 ls fixed