# HG changeset patch # User bgruening # Date 1479227028 18000 # Node ID c778eb2f721bd396ed06d95a1ed1f6f5c93d2cf1 # Parent eb06835653b0e79d0fea86035d36accb6a288043 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit 49aa400dbbcab6ce76272d8b05df6981a5307783 diff -r eb06835653b0 -r c778eb2f721b README.rst --- a/README.rst Wed Nov 02 10:27:57 2016 -0400 +++ b/README.rst Tue Nov 15 11:23:48 2016 -0500 @@ -1,15 +1,16 @@ -Galaxy wrappers for sailfish +Galaxy wrappers for salmon ===================================== -These wrappers are copyright 2014 by Björn Grüning, Mhd Ramez Alrawas and additional contributors. +These wrappers are copyright 2014, updated 2016 by Björn Grüning, Mhd Ramez Alrawas and additional contributors. All rights reserved. See the licence text below. -Currently tested with sailfish 0.10.1 +Currently tested with salmon 0.7.2. + Automated Installation ====================== -Galaxy should be able to automatically install the sailfish for you. +Galaxy should be able to automatically install the salmon for you. History @@ -20,7 +21,8 @@ -------- ---------------------------------------------------------------------- v0.6.3.0 - First release -------- ---------------------------------------------------------------------- -v0.10.1 - Second release +v0.7.2 - Second release + ======== ====================================================================== diff -r eb06835653b0 -r c778eb2f721b sailfish.tar.bz2 Binary file sailfish.tar.bz2 has changed diff -r eb06835653b0 -r c778eb2f721b sailfish.xml --- a/sailfish.xml Wed Nov 02 10:27:57 2016 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,429 +0,0 @@ - - transcript quantification from RNA-seq data - - - - - - - - - - - sailfish - - - - - - - - - sailfish -version - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - geneMap - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - `_ RNA-seq - read aligner has a different convention for specifying the format of the library. - Below is a table that provides the corresponding sailfish/salmon library format - string for each of the potential TopHat library types: - - - +---------------------+-------------------------+ - | TopHat | Salmon (and Sailfish) | - +=====================+============+============+ - | | Paired-end | Single-end | - +---------------------+------------+------------+ - |``-fr-unstranded`` |``-l IU`` |``-l U`` | - +---------------------+------------+------------+ - |``-fr-firststrand`` |``-l ISR`` |``-l SR`` | - +---------------------+------------+------------+ - |``-fr-secondstrand`` |``-l ISF`` |``-l SF`` | - +---------------------+------------+------------+ - - The remaining salmon library format strings are not directly expressible in terms - of the TopHat library types, and so there is no direct mapping for them. - - - ]]> - - 10.1038/nbt.2862 - - diff -r eb06835653b0 -r c778eb2f721b salmon.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/salmon.xml Tue Nov 15 11:23:48 2016 -0500 @@ -0,0 +1,486 @@ + + + Transcript Quantification from RNA-seq data + + + + + + + + + + + + + salmon + + + + + + + + + + salmon -version + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+
+ + + + + geneMap + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `_ RNA-seq + read aligner has a different convention for specifying the format of the library. + Below is a table that provides the corresponding Salmon/salmon library format + string for each of the potential TopHat library types: + + +---------------------+-------------------------+ + | TopHat | Salmon (and Sailfish) | + +=====================+============+============+ + | | Paired-end | Single-end | + +---------------------+------------+------------+ + |``-fr-unstranded`` |``-l IU`` |``-l U`` | + +---------------------+------------+------------+ + |``-fr-firststrand`` |``-l ISR`` |``-l SR`` | + +---------------------+------------+------------+ + |``-fr-secondstrand`` |``-l ISF`` |``-l SF`` | + +---------------------+------------+------------+ + + The remaining salmon library format strings are not directly expressible in terms + of the TopHat library types, and so there is no direct mapping for them. +]]> + + 10.1101/021592 + +
diff -r eb06835653b0 -r c778eb2f721b test-data/gene_map.tab --- a/test-data/gene_map.tab Wed Nov 02 10:27:57 2016 -0400 +++ b/test-data/gene_map.tab Tue Nov 15 11:23:48 2016 -0500 @@ -1,3 +1,4 @@ NM_174914 foo NM_001168316 bar NR_003084 baz +NR_031764 foo diff -r eb06835653b0 -r c778eb2f721b test-data/sailfish_bias_result1.tab --- a/test-data/sailfish_bias_result1.tab Wed Nov 02 10:27:57 2016 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,16 +0,0 @@ -Name Length EffectiveLength TPM NumReads -NM_001168316 2283 1528.95 12702.4 158.926 -NM_174914 2385 1599.63 114719 1501.66 -NR_031764 1853 1214.33 10407.1 103.415 -NM_004503 1681 1085.83 37300.1 331.428 -NM_006897 1541 984.724 82401.9 664 -NM_014212 2037 1316.12 5106.81 55 -NM_014620 2300 1541.27 46908.6 591.628 -NM_017409 1959 1273.24 4510.99 47 -NM_017410 2396 1562.29 3285.28 42 -NM_018953 1612 1019.15 27338 227.994 -NM_022658 2288 1634.87 364846 4881 -NM_153633 1666 1082.85 40694.7 360.597 -NM_153693 2072 1374.67 6520.1 73.3448 -NM_173860 849 483.271 243258 962 -NR_003084 1640 1052.77 1.09566 0.00943897 diff -r eb06835653b0 -r c778eb2f721b test-data/sailfish_genMap_result1.tab --- a/test-data/sailfish_genMap_result1.tab Wed Nov 02 10:27:57 2016 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,5 +0,0 @@ -Name Length EffectiveLength TPM NumReads -baz 1640 1052.77 1.09566 0.00943897 -NR_031764 1853 1214.33 10407.1 103.415 -foo 348.949 235.269 976889 9737.65 -bar 2283 1528.95 12702.4 158.926 diff -r eb06835653b0 -r c778eb2f721b test-data/sailfish_quant_result1.tab --- a/test-data/sailfish_quant_result1.tab Wed Nov 02 10:27:57 2016 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,16 +0,0 @@ -Name Length EffectiveLength TPM NumReads -NM_001168316 2283 2082.61 12552.5 161.366 -NM_174914 2385 2184.61 111020 1497.1 -NR_031764 1853 1652.61 10345.6 105.535 -NM_004503 1681 1480.61 36162.7 330.503 -NM_006897 1541 1340.61 80240.2 664 -NM_014212 2037 1836.61 4851.45 55 -NM_014620 2300 2099.61 45082 584.273 -NM_017409 1959 1758.61 4329.67 47 -NM_017410 2396 2195.61 3098.99 42 -NM_018953 1612 1411.61 26165.8 227.994 -NM_022658 2288 2087.61 378779 4881 -NM_153633 1666 1465.61 40626.6 367.539 -NM_153693 2072 1871.61 6464.46 74.683 -NM_173860 849 648.611 240280 962 -NR_003084 1640 1439.61 1.04309 0.00926914 diff -r eb06835653b0 -r c778eb2f721b test-data/salmon_genMap_result1.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/salmon_genMap_result1.tab Tue Nov 15 11:23:48 2016 -0500 @@ -0,0 +1,5 @@ +Name Length EffectiveLength TPM NumReads +baz 1640 1052.77 1.09566 0.00943897 +NR_031764 1853 1214.33 10407.1 103.415 +foo 348.949 235.269 976889 9737.65 +bar 2283 1528.95 12702.4 158.926 diff -r eb06835653b0 -r c778eb2f721b test-data/salmon_quant_result1.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/salmon_quant_result1.tab Tue Nov 15 11:23:48 2016 -0500 @@ -0,0 +1,16 @@ +Name Length EffectiveLength TPM NumReads +NM_001168316 2283 2106.09 12428 158.641 +NM_174914 2385 2208.09 112348 1503.55 +NR_031764 1853 1676.09 10021.5 101.805 +NM_004503 1681 1504.09 36259.8 330.551 +NM_006897 1541 1364.09 80313.1 664 +NM_014212 2037 1860.09 4878.54 55 +NM_014620 2300 2123.09 45879.2 590.368 +NM_017409 1959 1782.09 4351.4 47 +NM_017410 2396 2219.09 3122.74 42 +NM_018953 1612 1435.09 26213 228 +NM_022658 2288 2111.09 381473 4881 +NM_153633 1666 1489.09 40073.1 361.67 +NM_153693 2072 1895.09 6478.45 74.4115 +NM_173860 849 672.091 236161 962 +NR_003084 1640 1463.09 0.00490872 4.3529e-05 diff -r eb06835653b0 -r c778eb2f721b tool-data/sailfish_indexes.loc.sample --- a/tool-data/sailfish_indexes.loc.sample Wed Nov 02 10:27:57 2016 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,14 +0,0 @@ -# This is a sample file distributed with Galaxy that is used to define a -# list of sailfish indices, using three columns tab separated: -# -# sailfish_index_id{tab}Sailfish Index desciption{tab}/data/sailfish_indexes/sailfish_index_id/ -# -# An index can be created with the following command: -# -# sailfish index -# --transcripts your_transcripts.fasta -# --kmerSize 21 -# --out /data/sailfish_indexes/sailfish_index_id/ -# --threads 4 -# - diff -r eb06835653b0 -r c778eb2f721b tool_data_table_conf.xml.sample --- a/tool_data_table_conf.xml.sample Wed Nov 02 10:27:57 2016 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,6 +0,0 @@ - - - value, name, path - -
-