# HG changeset patch
# User bgruening
# Date 1479227028 18000
# Node ID c778eb2f721bd396ed06d95a1ed1f6f5c93d2cf1
# Parent eb06835653b0e79d0fea86035d36accb6a288043
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit 49aa400dbbcab6ce76272d8b05df6981a5307783
diff -r eb06835653b0 -r c778eb2f721b README.rst
--- a/README.rst Wed Nov 02 10:27:57 2016 -0400
+++ b/README.rst Tue Nov 15 11:23:48 2016 -0500
@@ -1,15 +1,16 @@
-Galaxy wrappers for sailfish
+Galaxy wrappers for salmon
=====================================
-These wrappers are copyright 2014 by Björn Grüning, Mhd Ramez Alrawas and additional contributors.
+These wrappers are copyright 2014, updated 2016 by Björn Grüning, Mhd Ramez Alrawas and additional contributors.
All rights reserved. See the licence text below.
-Currently tested with sailfish 0.10.1
+Currently tested with salmon 0.7.2.
+
Automated Installation
======================
-Galaxy should be able to automatically install the sailfish for you.
+Galaxy should be able to automatically install the salmon for you.
History
@@ -20,7 +21,8 @@
-------- ----------------------------------------------------------------------
v0.6.3.0 - First release
-------- ----------------------------------------------------------------------
-v0.10.1 - Second release
+v0.7.2 - Second release
+
======== ======================================================================
diff -r eb06835653b0 -r c778eb2f721b sailfish.tar.bz2
Binary file sailfish.tar.bz2 has changed
diff -r eb06835653b0 -r c778eb2f721b sailfish.xml
--- a/sailfish.xml Wed Nov 02 10:27:57 2016 -0400
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-
- transcript quantification from RNA-seq data
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- sailfish
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- sailfish -version
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- `_ RNA-seq
- read aligner has a different convention for specifying the format of the library.
- Below is a table that provides the corresponding sailfish/salmon library format
- string for each of the potential TopHat library types:
-
-
- +---------------------+-------------------------+
- | TopHat | Salmon (and Sailfish) |
- +=====================+============+============+
- | | Paired-end | Single-end |
- +---------------------+------------+------------+
- |``-fr-unstranded`` |``-l IU`` |``-l U`` |
- +---------------------+------------+------------+
- |``-fr-firststrand`` |``-l ISR`` |``-l SR`` |
- +---------------------+------------+------------+
- |``-fr-secondstrand`` |``-l ISF`` |``-l SF`` |
- +---------------------+------------+------------+
-
- The remaining salmon library format strings are not directly expressible in terms
- of the TopHat library types, and so there is no direct mapping for them.
-
-
- ]]>
-
- 10.1038/nbt.2862
-
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diff -r eb06835653b0 -r c778eb2f721b salmon.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/salmon.xml Tue Nov 15 11:23:48 2016 -0500
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+ Transcript Quantification from RNA-seq data
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+ salmon
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+ salmon -version
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+ `_ RNA-seq
+ read aligner has a different convention for specifying the format of the library.
+ Below is a table that provides the corresponding Salmon/salmon library format
+ string for each of the potential TopHat library types:
+
+ +---------------------+-------------------------+
+ | TopHat | Salmon (and Sailfish) |
+ +=====================+============+============+
+ | | Paired-end | Single-end |
+ +---------------------+------------+------------+
+ |``-fr-unstranded`` |``-l IU`` |``-l U`` |
+ +---------------------+------------+------------+
+ |``-fr-firststrand`` |``-l ISR`` |``-l SR`` |
+ +---------------------+------------+------------+
+ |``-fr-secondstrand`` |``-l ISF`` |``-l SF`` |
+ +---------------------+------------+------------+
+
+ The remaining salmon library format strings are not directly expressible in terms
+ of the TopHat library types, and so there is no direct mapping for them.
+]]>
+
+ 10.1101/021592
+
+
diff -r eb06835653b0 -r c778eb2f721b test-data/gene_map.tab
--- a/test-data/gene_map.tab Wed Nov 02 10:27:57 2016 -0400
+++ b/test-data/gene_map.tab Tue Nov 15 11:23:48 2016 -0500
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NM_174914 foo
NM_001168316 bar
NR_003084 baz
+NR_031764 foo
diff -r eb06835653b0 -r c778eb2f721b test-data/sailfish_bias_result1.tab
--- a/test-data/sailfish_bias_result1.tab Wed Nov 02 10:27:57 2016 -0400
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
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-Name Length EffectiveLength TPM NumReads
-NM_001168316 2283 1528.95 12702.4 158.926
-NM_174914 2385 1599.63 114719 1501.66
-NR_031764 1853 1214.33 10407.1 103.415
-NM_004503 1681 1085.83 37300.1 331.428
-NM_006897 1541 984.724 82401.9 664
-NM_014212 2037 1316.12 5106.81 55
-NM_014620 2300 1541.27 46908.6 591.628
-NM_017409 1959 1273.24 4510.99 47
-NM_017410 2396 1562.29 3285.28 42
-NM_018953 1612 1019.15 27338 227.994
-NM_022658 2288 1634.87 364846 4881
-NM_153633 1666 1082.85 40694.7 360.597
-NM_153693 2072 1374.67 6520.1 73.3448
-NM_173860 849 483.271 243258 962
-NR_003084 1640 1052.77 1.09566 0.00943897
diff -r eb06835653b0 -r c778eb2f721b test-data/sailfish_genMap_result1.tab
--- a/test-data/sailfish_genMap_result1.tab Wed Nov 02 10:27:57 2016 -0400
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
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-Name Length EffectiveLength TPM NumReads
-baz 1640 1052.77 1.09566 0.00943897
-NR_031764 1853 1214.33 10407.1 103.415
-foo 348.949 235.269 976889 9737.65
-bar 2283 1528.95 12702.4 158.926
diff -r eb06835653b0 -r c778eb2f721b test-data/sailfish_quant_result1.tab
--- a/test-data/sailfish_quant_result1.tab Wed Nov 02 10:27:57 2016 -0400
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
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-Name Length EffectiveLength TPM NumReads
-NM_001168316 2283 2082.61 12552.5 161.366
-NM_174914 2385 2184.61 111020 1497.1
-NR_031764 1853 1652.61 10345.6 105.535
-NM_004503 1681 1480.61 36162.7 330.503
-NM_006897 1541 1340.61 80240.2 664
-NM_014212 2037 1836.61 4851.45 55
-NM_014620 2300 2099.61 45082 584.273
-NM_017409 1959 1758.61 4329.67 47
-NM_017410 2396 2195.61 3098.99 42
-NM_018953 1612 1411.61 26165.8 227.994
-NM_022658 2288 2087.61 378779 4881
-NM_153633 1666 1465.61 40626.6 367.539
-NM_153693 2072 1871.61 6464.46 74.683
-NM_173860 849 648.611 240280 962
-NR_003084 1640 1439.61 1.04309 0.00926914
diff -r eb06835653b0 -r c778eb2f721b test-data/salmon_genMap_result1.tab
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/salmon_genMap_result1.tab Tue Nov 15 11:23:48 2016 -0500
@@ -0,0 +1,5 @@
+Name Length EffectiveLength TPM NumReads
+baz 1640 1052.77 1.09566 0.00943897
+NR_031764 1853 1214.33 10407.1 103.415
+foo 348.949 235.269 976889 9737.65
+bar 2283 1528.95 12702.4 158.926
diff -r eb06835653b0 -r c778eb2f721b test-data/salmon_quant_result1.tab
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/salmon_quant_result1.tab Tue Nov 15 11:23:48 2016 -0500
@@ -0,0 +1,16 @@
+Name Length EffectiveLength TPM NumReads
+NM_001168316 2283 2106.09 12428 158.641
+NM_174914 2385 2208.09 112348 1503.55
+NR_031764 1853 1676.09 10021.5 101.805
+NM_004503 1681 1504.09 36259.8 330.551
+NM_006897 1541 1364.09 80313.1 664
+NM_014212 2037 1860.09 4878.54 55
+NM_014620 2300 2123.09 45879.2 590.368
+NM_017409 1959 1782.09 4351.4 47
+NM_017410 2396 2219.09 3122.74 42
+NM_018953 1612 1435.09 26213 228
+NM_022658 2288 2111.09 381473 4881
+NM_153633 1666 1489.09 40073.1 361.67
+NM_153693 2072 1895.09 6478.45 74.4115
+NM_173860 849 672.091 236161 962
+NR_003084 1640 1463.09 0.00490872 4.3529e-05
diff -r eb06835653b0 -r c778eb2f721b tool-data/sailfish_indexes.loc.sample
--- a/tool-data/sailfish_indexes.loc.sample Wed Nov 02 10:27:57 2016 -0400
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
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-# This is a sample file distributed with Galaxy that is used to define a
-# list of sailfish indices, using three columns tab separated:
-#
-# sailfish_index_id{tab}Sailfish Index desciption{tab}/data/sailfish_indexes/sailfish_index_id/
-#
-# An index can be created with the following command:
-#
-# sailfish index
-# --transcripts your_transcripts.fasta
-# --kmerSize 21
-# --out /data/sailfish_indexes/sailfish_index_id/
-# --threads 4
-#
-
diff -r eb06835653b0 -r c778eb2f721b tool_data_table_conf.xml.sample
--- a/tool_data_table_conf.xml.sample Wed Nov 02 10:27:57 2016 -0400
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
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-
-
-