Mercurial > repos > bgruening > deeptools_plot_heatmap
comparison deepTools_macros.xml @ 9:f509604dbf06 draft
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 4f515024772311c950d277246db548453d24abd7
author | bgruening |
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date | Wed, 23 Dec 2015 14:38:05 -0500 |
parents | 8ed7e3b6a16c |
children | 5984ae55928f |
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8:8ed7e3b6a16c | 9:f509604dbf06 |
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53 | 53 |
54 | 54 |
55 <xml name="includeZeros"> | 55 <xml name="includeZeros"> |
56 <param argument="--includeZeros" type="boolean" truevalue="--includeZeros" falsevalue="" | 56 <param argument="--includeZeros" type="boolean" truevalue="--includeZeros" falsevalue="" |
57 label="Include zeros" | 57 label="Include zeros" |
58 help="If set, then regions with zero counts for *all* BAM files given are included. The default behavior is to ignore those cases." /> | 58 help="If set, then regions with zero counts for *all* BAM files are included. The default behavior is to ignore such regions." /> |
59 </xml> | 59 </xml> |
60 | 60 |
61 <xml name="zMin_zMax"> | 61 <xml name="zMin_zMax"> |
62 <param argument="--zMin" type="float" value="" optional="true" label="Minimum value for the heatmap intensities" | 62 <param argument="--zMin" type="float" value="" optional="true" label="Minimum value for the heatmap intensities" |
63 help="If not specified the value is set automatically."/> | 63 help="If not specified the value is set automatically."/> |
66 </xml> | 66 </xml> |
67 | 67 |
68 <xml name="region_limit_operation"> | 68 <xml name="region_limit_operation"> |
69 <param argument="--region" type="text" value="" | 69 <param argument="--region" type="text" value="" |
70 label="Region of the genome to limit the operation to" | 70 label="Region of the genome to limit the operation to" |
71 help="This is useful when testing parameters to reduce the computing time. The format is chr:start:end, for example "chr10" or "chr10:456700:891000"." /> | 71 help="This is useful when testing parameters to reduce the time required. The format is chr:start:end, for example "chr10" or "chr10:456700:891000"." /> |
72 </xml> | 72 </xml> |
73 | 73 |
74 <token name="@THREADS@">--numberOfProcessors "\${GALAXY_SLOTS:-4}"</token> | 74 <token name="@THREADS@">--numberOfProcessors "\${GALAXY_SLOTS:-4}"</token> |
75 <token name="@WRAPPER_VERSION@">2.0.0</token> | 75 <token name="@WRAPPER_VERSION@">2.0.0</token> |
76 <xml name="requirements"> | 76 <xml name="requirements"> |
84 <version_command>@BINARY@ --version</version_command> | 84 <version_command>@BINARY@ --version</version_command> |
85 </xml> | 85 </xml> |
86 | 86 |
87 <xml name="smoothLength"> | 87 <xml name="smoothLength"> |
88 <param argument="--smoothLength" type="integer" value="" optional="True" min="1" | 88 <param argument="--smoothLength" type="integer" value="" optional="True" min="1" |
89 label="Smooth values using the following length (in bp)" | 89 label="Smooth values using the following length (in bases)" |
90 help ="The smooth length defines a window, larger than the bin size, to average the number of reads. For example, if the bin size is set to 20 bp and the smooth length is set to 60 bp, then, for each bin size the average of it and its left and right neighbors is considered. Any value smaller than the bin size will be ignored and no smoothing will be applied."/> | 90 help ="The smooth length defines a window, larger than the bin size, over which the number of reads is to be averaged. For example, if the bin size is set to 20 and the smooth length is 60, then, for each bin, its value is set to the average of it and its left and right neighbors. Any value smaller than the bin size will be ignored and no smoothing will be applied."/> |
91 </xml> | 91 </xml> |
92 | 92 |
93 | 93 |
94 <xml name="kmeans_clustering"> | 94 <xml name="kmeans_clustering"> |
95 <conditional name="used_multiple_regions"> | 95 <conditional name="used_multiple_regions"> |
105 <option value="none">No clustering</option> | 105 <option value="none">No clustering</option> |
106 <option value="kmeans">Kmeans clustering</option> | 106 <option value="kmeans">Kmeans clustering</option> |
107 </param> | 107 </param> |
108 <when value="kmeans"> | 108 <when value="kmeans"> |
109 <param name="k_kmeans" type="integer" value="0" label="Number of clusters to compute" | 109 <param name="k_kmeans" type="integer" value="0" label="Number of clusters to compute" |
110 help="When this option is set, then the matrix is split into clusters using the kmeans algorithm. | 110 help="When this option is set, the matrix is split into clusters using the k-means algorithm. |
111 Only works for data that is not grouped, otherwise only the first group will be clustered. | 111 This only works for data that is not grouped, otherwise only the first group will be clustered. |
112 If more specific clustering methods are required it is advisable to save the underlying matrix and | 112 If more specific clustering methods are required it is advisable to save the underlying matrix and |
113 run the clustering using other software. The plotting of the clustering may fail (Error: Segmentation fault) | 113 run the clustering using other software."/> |
114 if a cluster has very few members compared to the total number or regions. (default: 0 [do not cluster])."/> | |
115 </when> | 114 </when> |
116 <when value="none" /> | 115 <when value="none" /> |
117 </conditional> | 116 </conditional> |
118 </when> | 117 </when> |
119 <when value="yes" /> | 118 <when value="yes" /> |
155 | 154 |
156 <xml name="extendReads"> | 155 <xml name="extendReads"> |
157 <conditional name="doExtendCustom"> | 156 <conditional name="doExtendCustom"> |
158 <param name="doExtend" type="select" label="Extend reads to the given average fragment size." | 157 <param name="doExtend" type="select" label="Extend reads to the given average fragment size." |
159 help="(1) Single-end reads and singletons are extended to match this length. (2) Paired-end reads are extended to match the fragment size, regardless of what is set here. | 158 help="(1) Single-end reads and singletons are extended to match this length. (2) Paired-end reads are extended to match the fragment size, regardless of what is set here. |
160 By default *each* read mate is extended. | 159 By default *each* read mate is extended. |
161 This can be modified using the SAM flags (see --samFlagInclude and --samFlagExclude options) to keep only the first or the second mate. | 160 This can be modified using the SAM flags (see --samFlagInclude and --samFlagExclude options) to keep only the first or the second mate. |
162 Unmated reads, mate reads that map on different chromosomes or too far apart are extended to the given value. | 161 Unmated reads, mate reads that map to different chromosomes or too far apart are extended to the given value. |
163 Reads are only extended if --extendReads is set to a value greater than the read length. *NOTE*: For spliced-read data, this option is not | 162 Reads are only extended if --extendReads is set to a value greater than the read length. *NOTE*: For spliced-read data, this option is not |
164 recommended as it will extend reads over skipped regions, e.g. introns in RNA-seq data."> | 163 recommended as it will extend reads over skipped regions, e.g. introns in RNA-seq data."> |
165 <option value="no" selected="True">No extension. The default value and most typically appropriate.</option> | 164 <option value="no" selected="True">No extension. The default value and most typically appropriate.</option> |
166 <option value="yes">Paired-end extension. Suitable only for paired-end datasets.</option> | 165 <option value="yes">Paired-end extension. Suitable only for paired-end datasets.</option> |
167 <option value="custom">A custom length, which will be applied to ALL reads.</option> | 166 <option value="custom">A custom length, which will be applied to ALL reads.</option> |
168 </param> | 167 </param> |
169 <when value="no" /> | 168 <when value="no" /> |
187 <param argument="--distanceBetweenBins" type="integer" value="0" min="0" | 186 <param argument="--distanceBetweenBins" type="integer" value="0" min="0" |
188 label="Distance between bins" | 187 label="Distance between bins" |
189 help="By default, bamCorrelate considers consecutive bins of | 188 help="By default, bamCorrelate considers consecutive bins of |
190 the specified 'Bin size'. However, to reduce the | 189 the specified 'Bin size'. However, to reduce the |
191 computation time, a larger distance between bins can | 190 computation time, a larger distance between bins can |
192 by given. Larger distances result in less bins being | 191 be given. Larger distances result in fewer bins being |
193 considered."/> | 192 considered."/> |
194 </xml> | 193 </xml> |
195 | 194 |
196 <xml name="centerReads"> | 195 <xml name="centerReads"> |
197 <param argument="--centerReads" type="boolean" truevalue="--centerReads" falsevalue="" | 196 <param argument="--centerReads" type="boolean" truevalue="--centerReads" falsevalue="" |
198 label="Center regions with respect to the fragment length" | 197 label="Center regions with respect to the fragment length" |
199 help="For paired-end data, the read is centered at the fragment length defined by the two ends of the fragment. For single-end data, the given fragment length is used. This option is useful to get a sharper signal around enriched regions. "/> | 198 help="For paired-end data the fragment is defined by the bounds of the reads. For single-end data the bounds are defined by the read and the user-definable fragment/extension length. This option is useful to get a sharper signal around enriched regions."/> |
200 </xml> | 199 </xml> |
201 | 200 |
202 <xml name="ignoreDuplicates"> | 201 <xml name="ignoreDuplicates"> |
203 <param argument="--ignoreDuplicates" type="boolean" truevalue="--ignoreDuplicates" falsevalue="" | 202 <param argument="--ignoreDuplicates" type="boolean" truevalue="--ignoreDuplicates" falsevalue="" |
204 label="Ignore duplicates" | 203 label="Ignore duplicates" |
227 </xml> | 226 </xml> |
228 | 227 |
229 <xml name="minMappingQuality"> | 228 <xml name="minMappingQuality"> |
230 <param argument="--minMappingQuality" type="integer" optional="true" value="1" min="1" | 229 <param argument="--minMappingQuality" type="integer" optional="true" value="1" min="1" |
231 label="Minimum mapping quality" | 230 label="Minimum mapping quality" |
232 help= "If set, only reads that have a mapping quality score higher than the given value are considered. *Note* Bowtie's Mapping quality is related to uniqueness: the higher the score, the more unique is a read. A mapping quality defined by Bowtie of 10 or less indicates that there is at least a 1 in 10 chance that the read truly originated elsewhere."/> | 231 help= "If set, only reads with a mapping quality score higher than this value are considered."/> |
233 </xml> | 232 </xml> |
234 | 233 |
235 <xml name="skipZeros"> | 234 <xml name="skipZeros"> |
236 <param argument="--skipZeros" type="boolean" truevalue="--skipZeros" falsevalue="" | 235 <param argument="--skipZeros" type="boolean" truevalue="--skipZeros" falsevalue="" |
237 label ="Skip zeros" | 236 label ="Skip zeros" |
238 help ="If set, then zero counts that happen for *all* BAM files given are ignored. This might have the effect that fewer regions are considered than indicated in the option where the number of samples is defined." /> | 237 help ="If set, then zero counts that happen for *all* BAM files given are ignored. This may result in fewer considered regions." /> |
239 </xml> | 238 </xml> |
240 | 239 |
241 <xml name="fragmentLength"> | 240 <xml name="fragmentLength"> |
242 <param argument="--fragmentLength" type="integer" value="300" min="1" | 241 <param argument="--fragmentLength" type="integer" value="300" min="1" |
243 label="Fragment length used for the sequencing" | 242 label="Fragment length used for the sequencing" |
244 help ="If paired-end reads are used, the fragment length is computed from the BAM file."/> | 243 help ="If paired-end reads are used, the fragment length is computed from the BAM file, so this is only needed for single-end data."/> |
245 </xml> | 244 </xml> |
246 | 245 |
247 <xml name="scaleFactor"> | 246 <xml name="scaleFactor"> |
248 <param argument="--scaleFactor" type="float" value="1" label="Scaling factor" | 247 <param argument="--scaleFactor" type="float" value="1" label="Scaling factor" |
249 help="When used in combination with --normalizeTo1x or | 248 help="When used in combination with --normalizeTo1x or |
300 </xml> | 299 </xml> |
301 | 300 |
302 <xml name="multiple_input_bigwigs"> | 301 <xml name="multiple_input_bigwigs"> |
303 <param argument="--bigwigfiles" type="data" format="bigwig" multiple="True" min="2" | 302 <param argument="--bigwigfiles" type="data" format="bigwig" multiple="True" min="2" |
304 label="Bigwig file" | 303 label="Bigwig file" |
305 help="The Bigwig file must be sorted."/> | 304 help="A Bigwig file."/> |
306 </xml> | 305 </xml> |
307 | 306 |
308 <xml name="plotTitle"> | 307 <xml name="plotTitle"> |
309 <param argument="--plotTitle" type="text" value="" size="30" optional="True" | 308 <param argument="--plotTitle" type="text" value="" size="30" optional="True" |
310 label="Title of the plot" | 309 label="Title of the plot" |
392 help="This parameter determines if non-covered regions | 391 help="This parameter determines if non-covered regions |
393 (regions without overlapping reads) in a bam/bigWig file | 392 (regions without overlapping reads) in a bam/bigWig file |
394 should be skipped. The default is to treat those | 393 should be skipped. The default is to treat those |
395 regions as having a value of zero. The decision to | 394 regions as having a value of zero. The decision to |
396 skip non-covered regions depends on the interpretation | 395 skip non-covered regions depends on the interpretation |
397 of the data. Non-covered regions may represent for | 396 of the data. Non-covered regions may represent, for |
398 example repetitive regions that want to be skipped. | 397 example, repetitive regions that should be ignored. |
399 (default: False)" /> | 398 (default: False)" /> |
400 </xml> | 399 </xml> |
401 | 400 |
402 <xml name="input_save_matrix_values"> | 401 <xml name="input_save_matrix_values"> |
403 <param argument="--saveMatrix" type="boolean" label="Save the matrix of values underlying the heatmap"/> | 402 <param argument="--saveMatrix" type="boolean" label="Save the matrix of values underlying the heatmap"/> |