Mercurial > repos > bgruening > deeptools_plot_fingerprint
annotate plotFingerprint.xml @ 22:d92782646040 draft
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit e071ca1cc84da473ecc209c9d5e633d90cbc6a78-dirty
author | bgruening |
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date | Tue, 11 Jul 2017 04:59:05 -0400 |
parents | d0390db2a666 |
children | 49bf0a3db1d7 |
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0
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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1 <tool id="deeptools_plot_fingerprint" name="plotFingerprint" version="@WRAPPER_VERSION@.0"> |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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2 <description>plots profiles of BAM files; useful for assesing ChIP signal strength</description> |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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3 <macros> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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4 <token name="@BINARY@">plotFingerprint</token> |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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5 <import>deepTools_macros.xml</import> |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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6 </macros> |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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7 <expand macro="requirements" /> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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8 <command> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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9 <![CDATA[ |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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10 @multiple_input_bams@ |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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11 @BINARY@ |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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12 @THREADS@ |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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13 --bamfiles #echo " ".join($files) |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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14 --labels #echo " ".join($labels) |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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15 --plotFile $outFileName |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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16 |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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17 #if $output.showOutputSettings == "yes" |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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18 --plotFileFormat $output.outFileFormat |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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19 #if $output.saveRawCounts: |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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20 --outRawCounts '$outFileRawCounts' |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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21 #end if |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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22 #if $output.saveQualityMetrics: |
2768f00a7d25
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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23 --outQualityMetrics '$outFileQualityMetrics' |
2768f00a7d25
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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24 #if $output.JSDsample: |
2768f00a7d25
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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25 #if "'{}'".format($output.JSDsample.display_name) in $labels: |
2768f00a7d25
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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26 #set JSDidx = $labels.index("'{}'".format($output.JSDsample.display_name)) |
20
d0390db2a666
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b5fc4073c884a2d6a4092ea85150db1971010f28
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27 --JSDsample "${JSDidx}.bam" |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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28 #end if |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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29 #end if |
2768f00a7d25
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 0eeb2e1a184186ba5ac044136e71b943e2af0f09
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30 #end if |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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31 #else |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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32 --plotFileFormat 'png' |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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33 #end if |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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34 |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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35 #if str($region).strip() != '': |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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36 --region '$region' |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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37 #end if |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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38 |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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39 #if $advancedOpt.showAdvancedOpt == "yes": |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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40 --binSize '$advancedOpt.binSize' |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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41 --numberOfSamples '$advancedOpt.numberOfSamples' |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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42 |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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43 $advancedOpt.ignoreDuplicates |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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44 $advancedOpt.skipZeros |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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45 |
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46 #if $advancedOpt.plotTitle and str($advancedOpt.plotTitle.value) != "": |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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47 --plotTitle '$advancedOpt.plotTitle' |
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48 #end if |
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49 @ADVANCED_OPTS_READ_PROCESSING@ |
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50 @blacklist@ |
0
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51 #end if |
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52 ]]> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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53 </command> |
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54 |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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55 <inputs> |
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56 <expand macro="multiple_input_bams" MIN="1"/> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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57 <expand macro="region_limit_operation" /> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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58 |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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59 <conditional name="advancedOpt"> |
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60 <param name="showAdvancedOpt" type="select" label="Show advanced options" > |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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61 <option value="no" selected="true">no</option> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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62 <option value="yes">yes</option> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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63 </param> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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64 <when value="no" /> |
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65 <when value="yes"> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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66 <param argument="--binSize" type="integer" value="500" min="1" |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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67 label="Bin size in bases" |
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68 help="Length in bases for a window used to sample the genome."/> |
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69 <param argument="--numberOfSamples" type="integer" value="100000" min="1" |
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70 label="Number of samples" |
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71 help="Number of samples taken from the genome to compute the scaling factors."/> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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72 <expand macro="read_processing_options" /> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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73 <expand macro="skipZeros" /> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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74 <expand macro="plotTitle" /> |
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75 <expand macro="blacklist" /> |
0
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76 </when> |
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77 </conditional> |
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78 |
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79 <conditional name="output"> |
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80 <param name="showOutputSettings" type="select" label="Show advanced output settings"> |
bb874a0121d5
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b'a4965adc865b4c85b35ed205bc10821edc104f20'
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81 <option value="no" selected="true">no</option> |
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82 <option value="yes">yes</option> |
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83 </param> |
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84 <when value="no" /> |
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85 <when value="yes"> |
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86 <expand macro="input_image_file_format" /> |
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87 <param name="saveRawCounts" type="boolean" label="Save the bin counts" help="(--outRawCounts)"/> |
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88 <param name="saveQualityMetrics" type="boolean" label="Save quality metrics" |
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89 help="The file will have one row per input BAM file and columns containing a variety of QC metrics. For the full description of all metrics and their interpretation, please see our online documentation: http://deeptools.readthedocs.io/en/latest/content/feature/plotFingerprint_QC_metrics.html. (--outQualityMetrics)"/> |
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90 <param name="JSDsample" type="data" format="bam" optional="true" |
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91 label="Jensen-Shannon distance reference sample" |
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92 help="If output metrics should be saved, then use this sample as the reference for computing the Jensen-Shannon distance and CHANCE metrics of all other samples. Normally this is an input sample. If you don't specify this, then these metrics will not be computed. This is only applicable if quality metrics are being saved. (--JSDsample)" /> |
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93 </when> |
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94 </conditional> |
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95 </inputs> |
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96 <outputs> |
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97 <expand macro="output_image_file_format" /> |
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98 <data format="tabular" name="outFileRawCounts" label="${tool.name} on ${on_string}: bin counts"> |
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99 <filter> |
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100 (( |
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101 output['showOutputSettings'] == 'yes' and |
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102 output['saveRawCounts'] is True |
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103 )) |
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104 </filter> |
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105 </data> |
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106 <data format="tabular" name="outFileQualityMetrics" label="${tool.name} on ${on_string}: quality metrics"> |
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107 <filter> |
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108 (( |
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109 output['showOutputSettings'] == 'yes' and |
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110 output['saveQualityMetrics'] is True |
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111 )) |
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112 </filter> |
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113 </data> |
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114 </outputs> |
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115 <tests> |
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116 <test> |
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117 <param name="bamfiles" value="bowtie2 test1.bam,bowtie2 test1.bam" ftype="bam" /> |
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118 <param name="showAdvancedOpt" value="no" /> |
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119 <param name="showOutputSettings" value="no" /> |
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120 <output name="outFileName" file="plotFingerprint_result1.png" ftype="png" compare="sim_size" /> |
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121 </test> |
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122 <test> |
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123 <param name="bamfiles" value="bowtie2 test1.bam,bowtie2 test1.bam" ftype="bam" /> |
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124 <param name="showAdvancedOpt" value="yes" /> |
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125 <param name="showOutputSettings" value="yes" /> |
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126 <param name="saveRawCounts" value="True" /> |
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127 <param name="saveQualityMetrics" value="True" /> |
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128 <param name="plotTitle" value="Test Fingerprint Plot" /> |
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129 <param name="JSDsample" value="bowtie2 test1.bam" ftype="bam" /> |
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130 <output name="outFileName" file="plotFingerprint_result2.png" ftype="png" compare="sim_size" /> |
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131 <output name="outFileRawCounts" file="plotFingerprint_result2.tabular" ftype="tabular" /> |
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132 <output name="outFileQualityMetrics" file="plotFingerprint_quality_metrics.tabular" ftype="tabular" /> |
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133 </test> |
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134 </tests> |
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135 <help> |
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136 <![CDATA[ |
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137 |
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138 |
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139 What it does |
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140 ------------ |
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141 |
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142 This tool is useful for assessing the strength of a ChIP (i.e. how clearly the enrichment signal can be separated from the background) |
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143 and is based on a method described in Diaz et al. (2012) Stat Appl Genet Mol Biol 11(3). |
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144 |
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145 |
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146 Output |
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147 ------ |
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148 |
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149 The default output is a diagnostic plot (see below for an example and further down for some background information). |
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150 |
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151 Optionally, you can obtain the table of raw values that were used to generate the plot. |
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152 |
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153 .. image:: $PATH_TO_IMAGES/plotFingerprint_output.png |
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154 :width: 600 |
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155 :height: 395 |
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156 |
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157 |
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158 Example plot |
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159 ------------- |
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160 |
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161 What follows is the output of ``plotFingerprint`` with our test ChIP-Seq data sets, limiting the analysis to chromosome X. Single-end reads were extended to |
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162 200 bp (advanced options). |
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164 .. image:: $PATH_TO_IMAGES/bamFP_galaxy_output.png |
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165 :width: 600 |
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166 :height: 450 |
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167 |
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168 ----- |
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169 |
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170 Theoretical Background |
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171 ---------------------- |
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172 |
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173 The tool first samples indexed BAM files and sums the per-base coverage of reads/fragments overlapping a window (bin) of the specified length. |
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174 These values are then sorted according to their rank (the bin with the highest number of reads has the highest rank) |
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175 and the cumulative sum plotted. An ideal input (control) with a uniform distribution of reads alignments |
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176 and infinite sequencing depth will result in a diagonal line. A very specific and strong ChIP enrichment, on the other hand, would result in a large portion |
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177 of reads accumulating in just a few bins and the resulting plot showing a steep rise toward the right-most edge. Such results are |
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178 most commonly seen with transcription factors. |
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179 |
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180 |
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181 .. image:: $PATH_TO_IMAGES/QC_fingerprint.png |
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182 :width: 600 |
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183 :height: 294 |
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184 |
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186 |
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187 @REFERENCES@ |
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188 ]]> |
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189 </help> |
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190 <expand macro="citations" /> |
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191 </tool> |