# HG changeset patch # User bgruening # Date 1450809900 18000 # Node ID ae54e08b15d7639b8de3f6271a9b07afb293c845 # Parent d5b93c88cc6b3e58d190e193fbbc242d78e66c14 planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 8b9cdb8dc4a8fedd2b2286e7afab6529e49a9d22-dirty diff -r d5b93c88cc6b -r ae54e08b15d7 deepTools_macros.xml --- a/deepTools_macros.xml Mon Dec 21 19:07:40 2015 -0500 +++ b/deepTools_macros.xml Tue Dec 22 13:45:00 2015 -0500 @@ -40,10 +40,10 @@ - #if $plotting_type.zMin: + #if str($plotting_type.zMin) != "": --zMin $plotting_type.zMin #end if - #if $plotting_type.zMax: + #if str($plotting_type.zMax) != "": --zMax $plotting_type.zMax #end if --colorMap '$plotting_type.colorMap' @@ -107,7 +107,11 @@ + help="When this option is set, then the matrix is split into clusters using the kmeans algorithm. + Only works for data that is not grouped, otherwise only the first group will be clustered. + If more specific clustering methods are required it is advisable to save the underlying matrix and + run the clustering using other software. The plotting of the clustering may fail (Error: Segmentation fault) + if a cluster has very few members compared to the total number or regions. (default: 0 [do not cluster])."/> @@ -408,7 +412,6 @@ - @@ -456,14 +459,6 @@ - - - (( - output['showOutputSettings'] == 'yes' and - output['saveData'] is True - )) - - (( diff -r d5b93c88cc6b -r ae54e08b15d7 plotCorrelation.xml --- a/plotCorrelation.xml Mon Dec 21 19:07:40 2015 -0500 +++ b/plotCorrelation.xml Tue Dec 22 13:45:00 2015 -0500 @@ -1,5 +1,5 @@ - creates a heatmap of correlation scores between different samples + creates a heatmap or scatterplot of correlation scores between different samples plotCorrelation deepTools_macros.xml @@ -91,7 +91,7 @@ **What it does** This tools takes a compressed matrix of scores (such as read coverages) for a number of genomic regions -and different samples. It can visualize the correlation among samples as scatterplots or as +and different samples. It can visualize the correlation among samples as scatterplots or as heatmap of correlation coefficients. Further output files are optional. The compressed input matrices are easily generated using the "bamCorrelate" and "bigwigCorrelate" modules of deeptools. @@ -105,10 +105,18 @@ **Output files**: -- **correlation structure**: a scatterplot of all mutual correlations between all samples in matrix -- **diagnostic plot**: clustered heatmap displaying the values for each pair-wise correlation, see below for an example +- **diagnostic plot**: Either a scatterplot or clustered heatmap (select above) displaying the values for each pair-wise correlation, + see below for an example - data matrix (optional): if you want to analyze or plot the correlation values using a different program, e.g. R, this matrix can be used +**Output with test dataset**: + +Following is the output of plotCorrelation with our test ChIP-Seq datasets. Average coverages were computed over 10kb bins for chromosome X, +from bigwig files using bigwigCorrelate. The output was used by plotCorrelation to make a heatmap of spearman correlation between samples. + +.. image:: $PATH_TO_IMAGES/plotCorrelation_galaxy_bw_heatmap_output.png + + ----- @REFERENCES@ diff -r d5b93c88cc6b -r ae54e08b15d7 static/images/plotCorrelation_galaxy_bw_heatmap_output.png Binary file static/images/plotCorrelation_galaxy_bw_heatmap_output.png has changed