view bigwigCompare.xml @ 21:89766a53cb44 draft

planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 3ea8716dd65a9abde96b69610be31571e4f0ae6d

<tool id="deeptools_bigwig_compare" name="bigwigCompare" version="@WRAPPER_VERSION@.0">
    <description>normalizes and compares two bigWig files to obtain the ratio, log2ratio or difference between them</description>
    <macros>
        <token name="@BINARY@">bigwigCompare</token>
        <import>deepTools_macros.xml</import>
    </macros>
    <expand macro="requirements"/>
    <command>
<![CDATA[
        @BINARY@
            @THREADS@
            --bigwig1 '$bigwigFile1'
            --bigwig2 '$bigwigFile2'

            --outFileName '$outFileName'
            --outFileFormat '$outFileFormat'

            --ratio $comparison.comparison_select

            #if $comparison.comparison_select in ['ratio','log2']:
                --pseudocount $comparison.pseudocount
            #end if

            #if str($region).strip() != '':
                --region '$region'
            #end if

            #if $advancedOpt.showAdvancedOpt == "yes":

              $advancedOpt.skipNAs
              --scaleFactors '$advancedOpt.scaleFactor1:$advancedOpt.scaleFactor2'
              --binSize $advancedOpt.binSize

                #if $advancedOpt.plotTitle and str($advancedOpt.plotTitle.value) != "":
                    --plotTitle '$advancedOpt.plotTitle'
                #end if

            #end if
]]>
    </command>
    <inputs>
        <param name="bigwigFile1" format="bigwig" type="data" label="Treatment bigwig file" />
        <param name="bigwigFile2" format="bigwig" type="data" label="bigWig file" />

        <conditional name="comparison">
            <param name="comparison_select" type="select" 
                label="How to compare the two files"
                help="The default is to output the log2ratio between the two samples.
                The reciprocal ratio returns the negative of the inverse of the ratio if
                the ratio is less than 0. The resulting values are interpreted as negative fold changes. (--ratio)">
                <option value="log2" selected="true">compute log2 of the number of reads ratio</option>
                <option value="ratio">compute the ratio of the number of reads</option>
                <option value="subtract">compute difference (subtract input from treatment) of the number of reads</option>
                <option value="add">compute the sum over all reads</option>
                <option value="reciprocal_ratio">Computes the fold change. If the fold change is less than 1, the negative of the inverse is reported. E.g. A fold change of 10 to 5 would be reported not as 0.5 but as -2</option>
            </param>
            <when value="log2">
                <expand macro="pseudocount" />
            </when>
            <when value="ratio">
                <expand macro="pseudocount" />
            </when>
            <when value="subtract" />
            <when value="add" />
            <when value="reciprocal_ratio">
                <expand macro="pseudocount" />
            </when>
        </conditional>

        <param name="outFileFormat" type="select" label="Coverage file format">
            <option value="bigwig" selected="true">bigwig</option>
            <option value="bedgraph">bedgraph</option>
        </param>

        <expand macro="region_limit_operation" />

        <conditional name="advancedOpt">
            <param name="showAdvancedOpt" type="select" label="Show advanced options" >
                <option value="no" selected="true">no</option>
                <option value="yes">yes</option>
            </param>
            <when value="no" />
            <when value="yes">
                <param argument="--binSize" type="integer" value="50" min="1" 
                    label="Length, in bases, of the non-overlapping bins used for averaging the score over the regions length"
                    help="Size of the bins in bases for the output of the bigwig/bedgraph file."/>

                <expand macro="skipNAs" />
                <expand macro="scaleFactors" />
                <expand macro="plotTitle" />
            </when>
        </conditional>
    </inputs>
    <outputs>
        <data format="bigwig" name="outFileName">
            <change_format>
                <when input="outFileFormat" value="bigwig" format="bigwig" />
                <when input="outFileFormat" value="bedgraph" format="bedgraph" />
            </change_format>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="bigwigFile1" value="test.bw" ftype="bigwig" />
            <param name="bigwigFile2" value="test.bw" ftype="bigwig" />
            <param name="showAdvancedOpt" value="no" />
            <param name="outFileFormat" value="bigwig" />
            <param name="binSize" value="5" />
            <param name="comparison_select" value="ratio" />
            <output name="outFileName" file="bigwigCompare_result1.bw" ftype="bigwig" />
        </test>
        <test>
            <param name="bigwigFile1" value="test.bw" ftype="bigwig" />
            <param name="bigwigFile2" value="test.bw" ftype="bigwig" />
            <param name="showAdvancedOpt" value="no" />
            <param name="outFileFormat" value="bedgraph" />
            <param name="binSize" value="10" />
            <param name="comparison_select" value="ratio" />
            <output name="outFileName" file="bigwigCompare_result2.bg" ftype="bedgraph" />
        </test>
    </tests>
    <help>
<![CDATA[

What it does
--------------

This tool compares two bigWig files based on the number of mapped reads. To
compare the bigwig files, the genome is partitioned into bins of equal size,
then the scores (e.g., number of reads) found in each bigWig file are counted for such bins and, finally, a summary value is reported.
This value can be the ratio of the number of reads per bin, the log2 of the ratio, the sum or the difference.

Note that you can actually produce a human-readable bedGraph format instead of the compressed bigWig format if you're interested in having a look at the values yourself.

.. image:: $PATH_TO_IMAGES/bigwigCompare_output.png
   :width: 600
   :height: 436

-----

@REFERENCES@
]]>
    </help>
    <expand macro="citations" />
</tool>