# HG changeset patch
# User bgruening
# Date 1450899657 18000
# Node ID ab153e5b542812dc44ad841546808c4dd3d22be3
# Parent b97024fb9c9f0d20d796b6cda09dae2e887c5d3e
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 4f515024772311c950d277246db548453d24abd7
diff -r b97024fb9c9f -r ab153e5b5428 bamFingerprint.xml
--- a/bamFingerprint.xml Wed Dec 23 07:31:18 2015 -0500
+++ b/bamFingerprint.xml Wed Dec 23 14:40:57 2015 -0500
@@ -58,8 +58,8 @@
+ label="Bin size in bases"
+ help="Length in bases for a window used to sample the genome."/>
@@ -115,16 +115,15 @@
**What it does**
-This tool is useful to assess the strength of a ChIP (i.e. how clearly the enrichment signal can be separated from the background signal)
-and it is based on a method developed by Diaz et al. (2012) Stat Appl Genet Mol Biol 11(3).
+This tool is useful for assessing the strength of a ChIP (i.e. how clearly the enrichment signal can be separated from the background)
+and is based on a method described in Diaz et al. (2012) Stat Appl Genet Mol Biol 11(3).
-The tool first samples indexed BAM files and counts all reads overlapping a window (bin) of specified length.
+The tool first samples indexed BAM files and counts all reads overlapping a window (bin) of the specified length.
These counts are then sorted according to their rank (the bin with the highest number of reads has the highest rank)
-and the cumulative sum of read counts are plotted. An ideal input (control sample) with perfect uniform distribution of reads
-along the genome (i.e. without enrichments in open chromatin etc.) should
-generate a straight diagonal line. A very specific and strong ChIP enrichment will be indicated by a prominent and steep
-rise of the cumulative sum towards the highest rank. This means that a big chunk of reads from the ChIP sample is located in
-few bins which corresponds to high, narrow enrichments seen for transcription factors.
+and the cumulative sum of read counts are plotted. An ideal input (control) with a uniform distribution of reads alignments
+result in a diagonal line. A very specific and strong ChIP enrichment, on the other hand, would result in a large portion
+of reads accumulating in just a few bins and the resulting plot showing a steep rise toward the right-most edge. Such results are
+most commonly seen with transcription factors.
.. image:: $PATH_TO_IMAGES/QC_fingerprint.png
@@ -140,7 +139,7 @@
**Output with test dataset**:
-Following is the output of bamFingerPrint with our test ChIP-Seq datasets, limiting analysis to chromosome X. Single-end reads were extended to
+What follows is the output of bamFingerPrint with our test ChIP-Seq datasets, limiting analysis to chromosome X. Single-end reads were extended to
200 bp (advanced options).
.. image:: $PATH_TO_IMAGES/bamFP_galaxy_output.png
diff -r b97024fb9c9f -r ab153e5b5428 deepTools_macros.xml
--- a/deepTools_macros.xml Wed Dec 23 07:31:18 2015 -0500
+++ b/deepTools_macros.xml Wed Dec 23 14:40:57 2015 -0500
@@ -55,7 +55,7 @@
+ help="If set, then regions with zero counts for *all* BAM files are included. The default behavior is to ignore such regions." />
@@ -68,7 +68,7 @@
+ help="This is useful when testing parameters to reduce the time required. The format is chr:start:end, for example "chr10" or "chr10:456700:891000"." />
--numberOfProcessors "\${GALAXY_SLOTS:-4}"
@@ -86,8 +86,8 @@
+ label="Smooth values using the following length (in bases)"
+ help ="The smooth length defines a window, larger than the bin size, over which the number of reads is to be averaged. For example, if the bin size is set to 20 and the smooth length is 60, then, for each bin, its value is set to the average of it and its left and right neighbors. Any value smaller than the bin size will be ignored and no smoothing will be applied."/>
@@ -107,11 +107,10 @@
+ run the clustering using other software."/>
@@ -157,11 +156,11 @@
+ By default *each* read mate is extended.
+ This can be modified using the SAM flags (see --samFlagInclude and --samFlagExclude options) to keep only the first or the second mate.
+ Unmated reads, mate reads that map to different chromosomes or too far apart are extended to the given value.
+ Reads are only extended if --extendReads is set to a value greater than the read length. *NOTE*: For spliced-read data, this option is not
+ recommended as it will extend reads over skipped regions, e.g. introns in RNA-seq data.">
@@ -189,14 +188,14 @@
help="By default, bamCorrelate considers consecutive bins of
the specified 'Bin size'. However, to reduce the
computation time, a larger distance between bins can
- by given. Larger distances result in less bins being
+ be given. Larger distances result in fewer bins being
considered."/>
+ help="For paired-end data the fragment is defined by the bounds of the reads. For single-end data the bounds are defined by the read and the user-definable fragment/extension length. This option is useful to get a sharper signal around enriched regions."/>
@@ -229,19 +228,19 @@
+ help= "If set, only reads with a mapping quality score higher than this value are considered."/>
+ help ="If set, then zero counts that happen for *all* BAM files given are ignored. This may result in fewer considered regions." />
+ help ="If paired-end reads are used, the fragment length is computed from the BAM file, so this is only needed for single-end data."/>
@@ -302,7 +301,7 @@
+ help="A Bigwig file."/>
@@ -394,8 +393,8 @@
should be skipped. The default is to treat those
regions as having a value of zero. The decision to
skip non-covered regions depends on the interpretation
- of the data. Non-covered regions may represent for
- example repetitive regions that want to be skipped.
+ of the data. Non-covered regions may represent, for
+ example, repetitive regions that should be ignored.
(default: False)" />