# HG changeset patch
# User bgruening
# Date 1457561643 18000
# Node ID b7e2129ad34825f563b54ac4cdd3c587202f17b5
# Parent 23bd2384fbca3819389fbf6a64a0fba3d02d7a10
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 2e8510e4f4015f51f7726de5697ba2de9b4e2f4c
diff -r 23bd2384fbca -r b7e2129ad348 bamCoverage.xml
--- a/bamCoverage.xml Thu Feb 18 11:32:16 2016 -0500
+++ b/bamCoverage.xml Wed Mar 09 17:14:03 2016 -0500
@@ -46,13 +46,17 @@
#if str($advancedOpt.ignoreForNormalization).strip() != '':
--ignoreForNormalization $advancedOpt.ignoreForNormalization
#end if
+
+ #if str($advancedOpt.filterRNAstrand) != 'no':
+ --filterRNAstrand '$advancedOpt.filterRNAstrand'
+ #end if
#end if
]]>
</command>
<inputs>
<param name="bamInput" format="bam" type="data" label="BAM file"
- help="The BAM file must be sorted."/>
+ help=""/>
<param name="binSize" type="integer" value="50" min="1"
label="Bin size in bases"
@@ -105,6 +109,14 @@
label="Determine nucleosome positions from MNase-seq data"
help="Only the 3 nucleotides at the center of each fragment are counted. The fragment ends are defined by the two mate reads. *NOTE*: Requires paired-end data." />
+ <param argument="filterRNAstrand" type="select" label="Only include reads originating from fragments from the forward or reverse strand."
+ help="By default (the no option), all reads are processed, regardless of the strand they originated from. For RNAseq, it can be useful to separately create bigWig files for the forward or reverse strands.
+ Note that this tools assumes that a dUTP-based method was used, so fragments will be assigned to the reverse strand if the second read in a pair is reverse complemented.">
+ <option value="no" selected="true">no</option>
+ <option value="forward">forward</option>
+ <option value="reverse">reverse</option>
+ </param>
+
</when>
</conditional>
</inputs>
@@ -153,6 +165,14 @@
<param name="binSize" value="10" />
<output name="outFileName" file="bamCoverage_result4.bg" ftype="bedgraph" />
</test>
+ <test>
+ <param name="bamInput" value="phiX.bam" ftype="bam" />
+ <param name="outFileFormat" value="bigwig" />
+ <param name="showAdvancedOpt" value="yes" />
+ <param name="filterRNAstrand" value="reverse" />
+ <param name="binSize" value="10" />
+ <output name="outFileName" file="bamCoverage_result5.bw" ftype="bigwig" />
+ </test>
</tests>
<help>
<![CDATA[
diff -r 23bd2384fbca -r b7e2129ad348 deepTools_macros.xml
--- a/deepTools_macros.xml Thu Feb 18 11:32:16 2016 -0500
+++ b/deepTools_macros.xml Wed Mar 09 17:14:03 2016 -0500
@@ -72,12 +72,12 @@
</xml>
<token name="@THREADS@">--numberOfProcessors "\${GALAXY_SLOTS:-4}"</token>
- <token name="@WRAPPER_VERSION@">2.1.0</token>
+ <token name="@WRAPPER_VERSION@">2.2.2</token>
<xml name="requirements">
<requirements>
<requirement type="package" version="2.7.10">python</requirement>
<requirement type="binary">@BINARY@</requirement>
- <requirement type="package" version="2.1.0">deepTools</requirement>
+ <requirement type="package" version="2.2.2">deepTools</requirement>
<yield />
</requirements>
<expand macro="stdio" />
@@ -310,13 +310,13 @@
<xml name="multiple_input_bams">
<param argument="--bamfiles" type="data" format="bam" min="1"
label="Bam file" multiple="true"
- help="The BAM file must be sorted."/>
+ help=""/>
</xml>
<xml name="multiple_input_bams_min2">
<param argument="--bamfiles" type="data" format="bam" min="2"
label="Bam file" multiple="true"
- help="The BAM file must be sorted."/>
+ help=""/>
</xml>
<xml name="multiple_input_bigwigs">
diff -r 23bd2384fbca -r b7e2129ad348 test-data/bamCoverage_result5.bw
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/computeMatrix_result1.gz
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/computeMatrix_result2.gz
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/computeMatrix_result3.gz
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/heatmapper_result1.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/heatmapper_result2.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/heatmapper_result2.tabular
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/heatmapper_result2.tabular Wed Mar 09 17:14:03 2016 -0500
@@ -0,0 +1,3 @@
+bin labels -0.0Kb 0.0Kb
+bins 1 2
+file_0 genes 2477942.34473 2610259.65234
diff -r 23bd2384fbca -r b7e2129ad348 test-data/plotCorrelation_result1.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/plotCoverage_result1.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/plotPCA_result1.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/plotPCA_result2.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/plotPCA_result2.tabular
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/plotPCA_result2.tabular Wed Mar 09 17:14:03 2016 -0500
@@ -0,0 +1,3 @@
+Component bowtie2-test1.bam bowtie2-test1.bam Eigenvalue
+1 -0.707106781187 -0.707106781187 6.0
+2 -0.707106781187 0.707106781187 1.23259516441e-32
diff -r 23bd2384fbca -r b7e2129ad348 test-data/profiler_result1.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/profiler_result2.png
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diff -r 23bd2384fbca -r b7e2129ad348 test-data/profiler_result2.tabular
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/profiler_result2.tabular Wed Mar 09 17:14:03 2016 -0500
@@ -0,0 +1,3 @@
+bin labels -0.0Kb 0.0Kb
+bins 1 2
+file_0 genes 2477942.34473 2610259.65234