# HG changeset patch # User bgruening # Date 1450899525 18000 # Node ID 9e8925f844449588dcad4875bb2079732561c54f # Parent a259f484f8849426ea13fc132cc50da552cdb770 planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 4f515024772311c950d277246db548453d24abd7 diff -r a259f484f884 -r 9e8925f84444 bamCoverage.xml --- a/bamCoverage.xml Wed Dec 23 07:29:21 2015 -0500 +++ b/bamCoverage.xml Wed Dec 23 14:38:45 2015 -0500 @@ -55,8 +55,8 @@ help="The BAM file must be sorted."/> + label="Bin size in bases" + help="The genome will be divided into bins of the specified size. For each bin, the overlaping number of fragments (or reads) will be reported. If only half a fragment overlaps, this fraction will be reported. "/> @@ -94,16 +94,16 @@ label="Regions that should be excluded for normalization" help="A list of chromosome names separated by spaces containing those chromosomes that should be excluded - for computing the normalization. This is useful when + during normalization. This is useful when considering samples with unequal coverage across - chromosomes like male samples. Example: chrX chrM" /> + chromosomes, like male and female samples. Example: chrX chrM" /> + help="Only the 3 nucleotides at the center of each fragment are counted. The fragment ends are defined by the two mate reads. *NOTE*: Requires paired-end data." /> @@ -163,7 +163,7 @@ number of reads (either extended to match the fragment length or not) that overlap each bin in the genome. The resulting read counts can be normalized using either a given scaling factor, the RPKM formula or to get a 1x depth of -coverage (RPGC). In the case of paired-end mapping each read mate is treated +coverage (RPGC). In the case of paired-end mapping, each read mate is treated independently to avoid a bias when a mixture of concordant and discordant pairs is present. This means that *each end* will be extended to match the fragment length. diff -r a259f484f884 -r 9e8925f84444 deepTools_macros.xml --- a/deepTools_macros.xml Wed Dec 23 07:29:21 2015 -0500 +++ b/deepTools_macros.xml Wed Dec 23 14:38:45 2015 -0500 @@ -55,7 +55,7 @@ + help="If set, then regions with zero counts for *all* BAM files are included. The default behavior is to ignore such regions." /> @@ -68,7 +68,7 @@ + help="This is useful when testing parameters to reduce the time required. The format is chr:start:end, for example "chr10" or "chr10:456700:891000"." /> --numberOfProcessors "\${GALAXY_SLOTS:-4}" @@ -86,8 +86,8 @@ + label="Smooth values using the following length (in bases)" + help ="The smooth length defines a window, larger than the bin size, over which the number of reads is to be averaged. For example, if the bin size is set to 20 and the smooth length is 60, then, for each bin, its value is set to the average of it and its left and right neighbors. Any value smaller than the bin size will be ignored and no smoothing will be applied."/> @@ -107,11 +107,10 @@ + run the clustering using other software."/> @@ -157,11 +156,11 @@ + By default *each* read mate is extended. + This can be modified using the SAM flags (see --samFlagInclude and --samFlagExclude options) to keep only the first or the second mate. + Unmated reads, mate reads that map to different chromosomes or too far apart are extended to the given value. + Reads are only extended if --extendReads is set to a value greater than the read length. *NOTE*: For spliced-read data, this option is not + recommended as it will extend reads over skipped regions, e.g. introns in RNA-seq data."> @@ -189,14 +188,14 @@ help="By default, bamCorrelate considers consecutive bins of the specified 'Bin size'. However, to reduce the computation time, a larger distance between bins can - by given. Larger distances result in less bins being + be given. Larger distances result in fewer bins being considered."/> + help="For paired-end data the fragment is defined by the bounds of the reads. For single-end data the bounds are defined by the read and the user-definable fragment/extension length. This option is useful to get a sharper signal around enriched regions."/> @@ -229,19 +228,19 @@ + help= "If set, only reads with a mapping quality score higher than this value are considered."/> + help ="If set, then zero counts that happen for *all* BAM files given are ignored. This may result in fewer considered regions." /> + help ="If paired-end reads are used, the fragment length is computed from the BAM file, so this is only needed for single-end data."/> @@ -302,7 +301,7 @@ + help="A Bigwig file."/> @@ -394,8 +393,8 @@ should be skipped. The default is to treat those regions as having a value of zero. The decision to skip non-covered regions depends on the interpretation - of the data. Non-covered regions may represent for - example repetitive regions that want to be skipped. + of the data. Non-covered regions may represent, for + example, repetitive regions that should be ignored. (default: False)" />