Mercurial > repos > bgruening > deeptools_bam_coverage
diff bamCoverage.xml @ 8:9e8925f84444 draft
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 4f515024772311c950d277246db548453d24abd7
author | bgruening |
---|---|
date | Wed, 23 Dec 2015 14:38:45 -0500 |
parents | a259f484f884 |
children | 300e98d37770 |
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--- a/bamCoverage.xml Wed Dec 23 07:29:21 2015 -0500 +++ b/bamCoverage.xml Wed Dec 23 14:38:45 2015 -0500 @@ -55,8 +55,8 @@ help="The BAM file must be sorted."/> <param name="binSize" type="integer" value="50" min="1" - label="Bin size in bp" - help="The genome will be divided in bins (also called tiles) of the specified length. For each bin the overlaping number of fragments (or reads) will be reported. If only half a fragment overlaps, this fraction will be reported. "/> + label="Bin size in bases" + help="The genome will be divided into bins of the specified size. For each bin, the overlaping number of fragments (or reads) will be reported. If only half a fragment overlaps, this fraction will be reported. "/> <conditional name="scaling"> <param name="type" type="select" label="Scaling/Normalization method" > @@ -94,16 +94,16 @@ label="Regions that should be excluded for normalization" help="A list of chromosome names separated by spaces containing those chromosomes that should be excluded - for computing the normalization. This is useful when + during normalization. This is useful when considering samples with unequal coverage across - chromosomes like male samples. Example: chrX chrM" /> + chromosomes, like male and female samples. Example: chrX chrM" /> <expand macro="skipNAs" /> <expand macro="read_processing_options" /> <param argument="--MNase" type="boolean" truevalue="--MNase" falsevalue="" label="Determine nucleosome positions from MNase-seq data" - help="Only 3 nucleotides at the center of each fragment are counted. The fragment ends are defined by the two mate reads. *NOTE*: Requires paired-end data." /> + help="Only the 3 nucleotides at the center of each fragment are counted. The fragment ends are defined by the two mate reads. *NOTE*: Requires paired-end data." /> </when> </conditional> @@ -163,7 +163,7 @@ number of reads (either extended to match the fragment length or not) that overlap each bin in the genome. The resulting read counts can be normalized using either a given scaling factor, the RPKM formula or to get a 1x depth of -coverage (RPGC). In the case of paired-end mapping each read mate is treated +coverage (RPGC). In the case of paired-end mapping, each read mate is treated independently to avoid a bias when a mixture of concordant and discordant pairs is present. This means that *each end* will be extended to match the fragment length.