# HG changeset patch # User bgruening # Date 1450899846 18000 # Node ID 3748f04eb047001a1e267d4c4b7c8957f380e899 # Parent c07969d63d7b7170ba304a38a198617be8f0eaec planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 4f515024772311c950d277246db548453d24abd7 diff -r c07969d63d7b -r 3748f04eb047 bamCompare.xml --- a/bamCompare.xml Wed Dec 23 07:33:54 2015 -0500 +++ b/bamCompare.xml Wed Dec 23 14:44:06 2015 -0500 @@ -1,5 +1,5 @@ - normalizes and compares two BAM files to obtain the ratio, log2ratio or difference + normalizes and compares two BAM files to obtain the ratio, log2ratio or difference between them bamCompare deepTools_macros.xml @@ -75,9 +75,9 @@ help="The BAM file must be sorted."/> + label="Bin size in bases" + help="The genome will be divided into bins of the specified size. For each bin, the overlaping number of fragments (or reads) will be reported. + If only half a fragment overlaps then this fraction will be reported."/> + For example, if you know of copy number variations between samples then you may want to exclude these. + Another typical example is the difference in chromosome X copies between males and females in many species. + Example inputs are chrX,chrY,chr3 or chr10:12220-128932" /> @@ -200,18 +200,18 @@ This tool compares two BAM files based on the number of mapped reads. To compare the BAM files, the genome is partitioned into bins of equal size, then -the number of reads found in each BAM file is counted for such bins and -finally a summarizing value is reported. This value can be the ratio of the +the number of reads found in each BAM file is counted per bin and +finally a summary value reported. This value can be the ratio of the number of reads per bin, the log2 of the ratio or the difference. This tool -can normalize the number of reads on each BAM file using the SES method -proposed by Diaz et al. (2012). "Normalization, bias correction, and peak +can normalize the number of reads in each BAM file using the SES method +proposed in Diaz et al. (2012). "Normalization, bias correction, and peak calling for ChIP-seq". Statistical applications in genetics and molecular biology, 11(3). Normalization based on read counts is also available. The output is either a bedgraph or a bigwig file containing the bin location and the resulting comparison values. By default, if reads are mated, the fragment -length reported in the BAM file is used. In the case of paired-end mapping +length reported in the BAM file is used. In the case of paired-end mapping, each read mate is treated independently to avoid a bias when a mixture of -concordant and discordant pairs is present. This means that *each end* will be +concordant and discordant pairs are present. This means that *each end* will be extended to match the fragment length. diff -r c07969d63d7b -r 3748f04eb047 deepTools_macros.xml --- a/deepTools_macros.xml Wed Dec 23 07:33:54 2015 -0500 +++ b/deepTools_macros.xml Wed Dec 23 14:44:06 2015 -0500 @@ -55,7 +55,7 @@ + help="If set, then regions with zero counts for *all* BAM files are included. The default behavior is to ignore such regions." /> @@ -68,7 +68,7 @@ + help="This is useful when testing parameters to reduce the time required. The format is chr:start:end, for example "chr10" or "chr10:456700:891000"." /> --numberOfProcessors "\${GALAXY_SLOTS:-4}" @@ -86,8 +86,8 @@ + label="Smooth values using the following length (in bases)" + help ="The smooth length defines a window, larger than the bin size, over which the number of reads is to be averaged. For example, if the bin size is set to 20 and the smooth length is 60, then, for each bin, its value is set to the average of it and its left and right neighbors. Any value smaller than the bin size will be ignored and no smoothing will be applied."/> @@ -107,11 +107,10 @@ + run the clustering using other software."/> @@ -157,11 +156,11 @@ + By default *each* read mate is extended. + This can be modified using the SAM flags (see --samFlagInclude and --samFlagExclude options) to keep only the first or the second mate. + Unmated reads, mate reads that map to different chromosomes or too far apart are extended to the given value. + Reads are only extended if --extendReads is set to a value greater than the read length. *NOTE*: For spliced-read data, this option is not + recommended as it will extend reads over skipped regions, e.g. introns in RNA-seq data."> @@ -189,14 +188,14 @@ help="By default, bamCorrelate considers consecutive bins of the specified 'Bin size'. However, to reduce the computation time, a larger distance between bins can - by given. Larger distances result in less bins being + be given. Larger distances result in fewer bins being considered."/> + help="For paired-end data the fragment is defined by the bounds of the reads. For single-end data the bounds are defined by the read and the user-definable fragment/extension length. This option is useful to get a sharper signal around enriched regions."/> @@ -229,19 +228,19 @@ + help= "If set, only reads with a mapping quality score higher than this value are considered."/> + help ="If set, then zero counts that happen for *all* BAM files given are ignored. This may result in fewer considered regions." /> + help ="If paired-end reads are used, the fragment length is computed from the BAM file, so this is only needed for single-end data."/> @@ -302,7 +301,7 @@ + help="A Bigwig file."/> @@ -394,8 +393,8 @@ should be skipped. The default is to treat those regions as having a value of zero. The decision to skip non-covered regions depends on the interpretation - of the data. Non-covered regions may represent for - example repetitive regions that want to be skipped. + of the data. Non-covered regions may represent, for + example, repetitive regions that should be ignored. (default: False)" />