Mercurial > repos > bgruening > deeptools
annotate computeMatrix.xml @ 73:bf8a2284232c draft
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit f720d773e34ad90d14d7cae77c1a0bb5d126102c-dirty
author | iuc |
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date | Fri, 18 Sep 2015 15:14:34 -0400 |
parents | 1dbd76a58d8b |
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61 | 1 <tool id="deeptools_computeMatrix" name="computeMatrix" version="@WRAPPER_VERSION@.0"> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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2 <description>preparation step to plot a heatmap or a profile</description> |
61 | 3 <macros> |
4 <token name="@BINARY@">computeMatrix</token> | |
5 <import>deepTools_macros.xml</import> | |
6 </macros> | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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7 <expand macro="requirements" /> |
61 | 8 <command> |
9 <![CDATA[ | |
10 #import tempfile | |
11 | |
12 #for $rf in $regionsFiles: | |
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13 cat "$rf.regionsFile" >> ./temp_input_path; |
61 | 14 #if str($rf.label.value).strip(): |
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15 echo "\#$rf.label.value" >> ./temp_input_path; |
61 | 16 #else: |
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9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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17 echo "\#$rf.regionsFile.name" >> ./temp_input_path; |
61 | 18 #end if |
19 #end for | |
20 | |
21 computeMatrix | |
22 | |
23 $mode.mode_select | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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24 --regionsFileName ./temp_input_path |
61 | 25 --scoreFileName '$scoreFile' |
26 --outFileName '$outFileName' | |
27 | |
28 @THREADS@ | |
29 | |
30 #if $output.showOutputSettings == "yes" | |
31 #if $output.saveData: | |
32 --outFileNameData '$outFileNameData' | |
33 #end if | |
34 #if $output.saveMatrix: | |
35 --outFileNameMatrix '$outFileNameMatrix' | |
36 #end if | |
37 | |
38 #if $output.saveSortedRegions: | |
39 --outFileSortedRegions '$outFileSortedRegions' | |
40 #end if | |
41 #end if | |
42 | |
43 #if $mode.mode_select == "reference-point": | |
44 --referencePoint $mode.referencePoint | |
45 $mode.nanAfterEnd | |
46 --beforeRegionStartLength $mode.beforeRegionStartLength | |
47 --afterRegionStartLength $mode.afterRegionStartLength | |
48 #else | |
49 --regionBodyLength $mode.regionBodyLength | |
50 --startLabel "$mode.startLabel" | |
51 --endLabel "$mode.endLabel" | |
52 #if $mode.regionStartLength.regionStartLength_select == "yes": | |
53 --beforeRegionStartLength $mode.regionStartLength.beforeRegionStartLength | |
54 --afterRegionStartLength $mode.regionStartLength.afterRegionStartLength | |
55 #end if | |
56 #end if | |
57 | |
58 #if $advancedOpt.showAdvancedOpt == "yes": | |
59 --sortRegions '$advancedOpt.sortRegions' | |
60 --sortUsing '$advancedOpt.sortUsing' | |
61 --averageTypeBins '$advancedOpt.averageTypeBins' | |
62 $advancedOpt.missingDataAsZero | |
63 $advancedOpt.skipZeros | |
64 --binSize $advancedOpt.binSize | |
65 | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit f720d773e34ad90d14d7cae77c1a0bb5d126102c-dirty
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66 #if $advancedOpt.minThreshold is not None and str($advancedOpt.minThreshold) != '': |
61 | 67 --minThreshold $advancedOpt.minThreshold |
68 #end if | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit f720d773e34ad90d14d7cae77c1a0bb5d126102c-dirty
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69 #if $advancedOpt.maxThreshold is not None and str($advancedOpt.maxThreshold) != '': |
61 | 70 --maxThreshold $advancedOpt.maxThreshold |
71 #end if | |
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1dbd76a58d8b
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit f720d773e34ad90d14d7cae77c1a0bb5d126102c-dirty
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72 #if $advancedOpt.scale is not None and str($advancedOpt.scale) != '': |
61 | 73 --scale $advancedOpt.scale |
74 #end if | |
75 | |
76 #end if | |
77 ]]> | |
78 </command> | |
79 <inputs> | |
80 | |
81 <repeat name="regionsFiles" title="regions to plot" min="1"> | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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82 <param name="regionsFile" format="bed" type="data" label="Regions to plot" |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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83 help="File, in BED format, containing the regions to plot."/> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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84 <param name="label" type="text" size="30" optional="true" value="" label="Label" |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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85 help="Label to use in the output."/> |
61 | 86 </repeat> |
87 | |
88 <param name="scoreFile" format="bigwig" type="data" | |
89 label="Score file" | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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90 help="Should be a bigWig file (containing a score, usually covering |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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91 the whole genome). You can generate a bigWig file either from a |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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92 bedGraph or WIG file using UCSC tools or from a BAM file using the |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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93 deepTool bamCoverage. (-scoreFile)"/> |
61 | 94 |
95 <conditional name="mode" > | |
96 <param name="mode_select" type="select" | |
97 label="computeMatrix has two main output options" | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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98 help="In the scale-regions mode, all regions in the BED file are |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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99 stretched or shrunk to the same length (bp) that is indicated |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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100 by the user. Reference-point refers to a position within the BED |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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101 regions (e.g start of region). In the reference-point mode only |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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102 those genomic positions before (downstream) and/or after (upstream) |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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103 the reference point will be plotted."> |
61 | 104 <option value="scale-regions" selected="true">scale-regions</option> |
105 <option value="reference-point">reference-point</option> | |
106 </param> | |
107 | |
108 <when value="scale-regions" > | |
109 <param name="regionBodyLength" type="integer" value="500" | |
110 label="Distance in bp to which all regions are going to be fitted" help="(--regionBodyLength)"/> | |
111 <param name="startLabel" type="text" value="TSS" size="10" | |
112 label="Label for the region start" | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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113 help ="Label shown in the plot for the start of the region. |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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114 Default is TSS (transcription start site), but could be changed to anything, |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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115 e.g. "peak start". (--startLabel)" /> |
61 | 116 <param name="endLabel" type="text" value="TES" size="10" |
117 label="Label for the region end" | |
118 help="Label shown in the plot for the region end. Default is TES (transcription end site). (--endLabel)"/> | |
119 <conditional name="regionStartLength"> | |
120 <param name="regionStartLength_select" type="select" label="Set distance up- and downstream of the given regions"> | |
121 <option value="no" selected="true">no</option> | |
122 <option value="yes">yes</option> | |
123 </param> | |
124 <when value="no" /> | |
125 <when value="yes"> | |
126 <param name="beforeRegionStartLength" type="integer" value="1000" min="1" | |
127 label="Distance upstream of the start site of the regions defined in the region file" | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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128 help="If the regions are genes, this would be the |
9bee2c86eeb1
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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129 distance upstream of the transcription start site. (--beforeRegionStartLength)"/> |
61 | 130 <param name="afterRegionStartLength" type="integer" value="1000" min="1" |
131 label="Distance downstream of the end site of the given regions" | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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132 help="If the regions are genes, this would be the |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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133 distance downstream of the transcription end site. (--afterRegionStartLength)"/> |
61 | 134 </when> |
135 </conditional> | |
136 </when> | |
137 <when value="reference-point"> | |
138 <param name="referencePoint" type="select" label="The reference point for the plotting"> | |
139 <option value="TSS" selected="true">beginning of region (e.g. TSS)</option> | |
140 <option value="TES">end of region (e.g. TES)</option> | |
141 <option value="center">center of region</option> | |
142 </param> | |
143 <param name="nanAfterEnd" type="boolean" truevalue="--nanAfterEnd" falsevalue="" | |
144 label="Discard any values after the region end" | |
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145 help="This is useful to visualize the region end when not using the |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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146 scale-regions mode and when the reference-point is set to the TSS. (--nanAfterEnd)"/> |
61 | 147 <param name="beforeRegionStartLength" type="integer" value="1000" min="1" |
148 label="Distance upstream of the start site of the regions defined in the region file" | |
149 help="If the regions are genes, this would be the distance upstream of the transcription start site. (--beforeRegionStartLength)"/> | |
150 <param name="afterRegionStartLength" type="integer" value="1000" min="1" | |
151 label="Distance downstream of the end site of the given regions" | |
152 help="If the regions are genes, this would be the distance downstream of the transcription end site. (--afterRegionStartLength)"/> | |
153 </when> | |
154 </conditional> | |
155 | |
156 <expand macro="input_graphic_output_settings"> | |
157 <expand macro="input_save_matrix_values" /> | |
158 </expand> | |
159 | |
160 <conditional name="advancedOpt" > | |
161 <param name="showAdvancedOpt" type="select" label="Show advanced options" > | |
162 <option value="no" selected="true">no</option> | |
163 <option value="yes">yes</option> | |
164 </param> | |
165 <when value="no" /> | |
166 <when value="yes"> | |
167 <param name="binSize" type="integer" value="10" min="1" | |
168 label="Length, in base pairs, of the non-overlapping bin for averaging the score over the regions length" | |
169 help="(--binSize)"/> | |
170 <param name="sortRegions" type="select" label="Sort regions" | |
171 help="Whether the output file should present the regions sorted."> | |
172 <option value="no" selected="true">no ordering</option> | |
173 <option value="descend">descending order</option> | |
174 <option value="ascend">ascending order</option> | |
175 </param> | |
176 | |
177 <param name="sortUsing" type="select" label="Method used for sorting" | |
178 help="The value is computed for each row. (--sortUsing)" > | |
179 <option value="mean" selected="true">mean</option> | |
180 <option value="median">median</option> | |
181 <option value="min">min</option> | |
182 <option value="max">max</option> | |
183 <option value="sum">sum</option> | |
184 <option value="region_length">region length</option> | |
185 </param> | |
186 | |
187 <param name="averageTypeBins" type="select" | |
188 label="Define the type of statistic that should be displayed." | |
189 help="The value is computed for each bin. (--averageTypeBins)"> | |
190 <option value="mean" selected="true">mean</option> | |
191 <option value="median">median</option> | |
192 <option value="min">min</option> | |
193 <option value="max">max</option> | |
194 <option value="sum">sum</option> | |
195 <option value="std">std</option> | |
196 </param> | |
197 | |
198 <param name="missingDataAsZero" type="boolean" truevalue="--missingDataAsZero" falsevalue="" | |
199 label="Indicate missing data as zero" | |
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200 help="Set to "yes", if missing data should be indicated as zeros. |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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201 Default is to ignore such cases which will be depicted as black areas in the |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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202 heatmap. (see "Missing data color" options of the heatmapper for additional options). |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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203 (--missingDataAsZero)"/> |
61 | 204 <param name="skipZeros" type="boolean" truevalue="--skipZeros" falsevalue="" |
205 label="Skip zeros" | |
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206 help="Whether regions with only scores of zero should be included or not. |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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207 Default is to include them. (--skipZeros)"/> |
61 | 208 <param name="minThreshold" type="float" optional="True" |
209 label="Minimum threshold" | |
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210 help="Any region containing a value that is equal or less than this numeric |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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211 value will be skipped. This is useful to skip, for example, genes where the |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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212 read count is zero for any of the bins. This could be the result of |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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213 unmappable areas and can bias the overall results. (--minThreshold)"/> |
61 | 214 <param name="maxThreshold" type="float" optional="True" |
215 label="Maximum threshold" | |
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216 help="Any region containing a value that is equal or higher that this |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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217 numeric value will be skipped. The max threshold is useful to skip those |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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218 few regions with very high read counts (e.g. major satellites) that may |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit ab1ab06323702186cf0c883d5774720cbb822cb5-dirty
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219 bias the average values. (--maxThreshold)"/> |
61 | 220 <param name="scale" type="float" optional="True" label="Scaling factor" |
221 help="If set, all values are multiplied by this number. (--scale)"/> | |
222 </when> | |
223 </conditional> | |
224 </inputs> | |
225 <outputs> | |
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226 <data format="deeptools_compute_matrix_archive" name="outFileName" label="${tool.name} on ${on_string}: Matrix" /> |
61 | 227 <expand macro="output_graphic_outputs" /> |
228 <expand macro="output_save_matrix_values" /> | |
229 </outputs> | |
230 <!-- | |
231 computeMatrix -S test.bw -R test2.bed -a 100 -b 100 -bs 1 | |
232 --> | |
233 <tests> | |
234 <test> | |
235 <param name="regionsFile" value="computeMatrix1.bed" ftype="bed" /> | |
236 <param name="scoreFile" value="bamCoverage_result4.bw" ftype="bigwig" /> | |
237 <param name="showAdvancedOpt" value="yes" /> | |
238 <param name="mode_select" value="reference-point" /> | |
239 <param name="binSize" value="10" /> | |
240 <param name="sortUsing" value="sum" /> | |
241 <param name="averageTypeBins" value="sum" /> | |
242 <param name="missingDataAsZero" value="True" /> | |
243 <param name="beforeRegionStartLength" value="10" /> | |
244 <param name="afterRegionStartLength" value="10" /> | |
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245 <output name="outFileName" file="computeMatrix_result1.gz" ftype="deeptools_compute_matrix_archive" compare="sim_size" /> |
61 | 246 </test> |
247 <test> | |
248 <param name="regionsFile" value="computeMatrix2.bed" ftype="bed" /> | |
249 <param name="scoreFile" value="computeMatrix2.bw" ftype="bigwig" /> | |
250 <param name="showAdvancedOpt" value="yes" /> | |
251 <param name="mode_select" value="reference-point" /> | |
252 <param name="binSize" value="10" /> | |
253 <param name="beforeRegionStartLength" value="10" /> | |
254 <param name="afterRegionStartLength" value="10" /> | |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit f720d773e34ad90d14d7cae77c1a0bb5d126102c-dirty
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255 <output name="outFileName" file="computeMatrix_result2.gz" ftype="deeptools_compute_matrix_archive" compare="sim_size" /> |
61 | 256 </test> |
257 </tests> | |
258 <help> | |
259 <![CDATA[ | |
260 **What it does** | |
261 | |
262 This tool prepares an intermediary file (a gzipped table of values) | |
263 that contains scores associated with genomic regions that can be used | |
264 afterwards to plot a heatmap or a profile. | |
265 | |
266 Genomic regions can really be anything - genes, parts of genes, ChIP-seq | |
267 peaks, favorite genome regions... as long as you provide a proper file | |
268 in BED or INTERVAL format. If you would like to compare different groups of regions | |
269 (i.e. genes from chromosome 2 and 3), you can supply more than 1 BED file, one for each group. | |
270 | |
271 computeMatrix can also be used to filter and sort | |
272 regions according to their score by making use of its advanced output options. | |
273 | |
274 | |
275 .. image:: $PATH_TO_IMAGES/flowChart_computeMatrixetc.png | |
276 :alt: Relationship between computeMatrix, heatmapper and profiler | |
277 | |
278 | |
279 You can find more details on the computeMatrix wiki page: https://github.com/fidelram/deepTools/wiki/Visualizations#wiki-computeMatrix | |
280 | |
281 | |
282 ----- | |
283 | |
284 @REFERENCES@ | |
285 ]]> | |
286 </help> | |
287 <expand macro="citations" /> | |
288 </tool> |