view getfastaBed.xml @ 5:b78d20957e7f draft

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<tool id="bedtools_getfastabed" name="GetFastaBed" version="0.2.0">
    <description></description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <expand macro="stdio" />
    <command>
        bedtools getfasta
        
        $name
        $tab
        $strand
        $split
        -fi $fasta 
        -bed $inputA
        -fo $output
    </command>
    <inputs>
        <param format="bed,vcf,gff,gff3" name="inputA" type="data" label="BED or BAM file"/>
        <param format="fasta" name="fasta" type="data" label="Fasta file"/>

        <param name="name" type="boolean" checked="false" truevalue="-name" falsevalue="" label="Use the “name” column in the BED file for the FASTA headers in the output FASTA file" />
        <param name="tab" type="boolean" checked="false" truevalue="-tab" falsevalue="" label="Report extract sequences in a tab-delimited format instead of in FASTA format." />
<param name="strand" type="boolean" checked="false" truevalue="-s" falsevalue="" label="Force strandedness." help="If the feature occupies the antisense strand, the sequence will be reverse complemented." />
<param name="split" type="boolean" checked="false" truevalue="-split" falsevalue="" label="Given BED12 input, extract and concatenate the sequences from the BED “blocks” (e.g., exons)" />
    </inputs>
    <outputs>
        <data format="fasta" name="output" />
    </outputs>
    <help>

**What it does**

bedtools getfasta will extract the sequence defined by the coordinates in a BED interval and create a new FASTA entry in the output file for each extracted sequence. By default, the FASTA header for each extracted sequence will be formatted as follows: “<chrom>:<start>-<end>”.

.. image:: $PATH_TO_IMAGES/getfasta-glyph.png

.. class:: warningmark

1. The headers in the input FASTA file must exactly match the chromosome column in the BED file.

2. You can use the UNIX fold command to set the line width of the FASTA output. For example, fold -w 60 will make each line of the FASTA file have at most 60 nucleotides for easy viewing.
    </help>
</tool>