# HG changeset patch
# User bebatut
# Date 1464855714 14400
# Node ID b0cd2569ccfbbe3a6fed1de62f0c090ce785db38
# Parent 7838137cbc7f4753efdbf2c39d90700f415b7b8f
planemo upload for repository https://github.com/ASaiM/galaxytools/tree/master/tools/humann2/ commit 79571d981d7d56657699be8aa24a40a36a8d0ab5-dirty
diff -r 7838137cbc7f -r b0cd2569ccfb README.rst
--- a/README.rst Thu Jun 02 02:31:20 2016 -0400
+++ b/README.rst Thu Jun 02 04:21:54 2016 -0400
@@ -2,10 +2,11 @@
===========================
Galaxy should be able to automatically install the dependencies, i.e. the
-MetaPhlAn2 binaries.
+HUMAnN2 binaries and its dependencies.
After installation, you must tell Galaxy about the defaut database with
-clade-specific marker genes, and where to find it:
+clade-specific marker genes used for taxonomic profiling (MetaPhlAn2), and where
+to find it:
* Put the ``metaphlan2_db.loc`` file in the ``tool-data/`` folder, after uncommenting last line
* Download whole MetaPhlan2 source code: https://bitbucket.org/biobakery/metaphlan2/get/2.5.0.zip
diff -r 7838137cbc7f -r b0cd2569ccfb humann2.xml
--- a/humann2.xml Thu Jun 02 02:31:20 2016 -0400
+++ b/humann2.xml Thu Jun 02 04:21:54 2016 -0400
@@ -38,15 +38,17 @@
&&
#end if
- #if $metaphlan2_db.metaphlan2_db_selector == "history"
- mkdir ref_db
- &&
- bowtie2-build $metaphlan2_db.metaphlan2_db_sequences ref_db/ref_db
- &&
- python $__tool_directory__/transform_json_to_pkl.py
- --json_input $metaphlan2_db_metadata
- --pkl_output ref_db/metadata.pkl
- &&
+ #if $taxonomic_profile.taxonomic_profile_test == "false"
+ #if $taxonomic_profile.metaphlan2_db_choice.metaphlan2_db_selector == "history"
+ `mkdir ref_db`
+ &&
+ bowtie2-build $taxonomic_profile.metaphlan2_db_choice.metaphlan2_db_sequences ref_db/ref_db
+ &&
+ python $__tool_directory__/transform_json_to_pkl.py
+ --json_input $metaphlan2_db_metadata
+ --pkl_output ref_db/metadata.pkl
+ &&
+ #end if
#end if
humann2
@@ -56,9 +58,9 @@
#if $taxonomic_profile.taxonomic_profile_test == "true":
--taxonomic-profile $taxonomic_profile.taxonomic_profile_file
#else
- #if $metaphlan2_db.metaphlan2_db_selector == "cached"
- #set $table = dict([(_[0], _[2]) for _ in $metaphlan2_db.cached_metaphlan2_db.input.options.tool_data_table.data])
- #set $db_choice = $metaphlan2_db.cached_metaphlan2_db.value
+ #if $taxonomic_profile.metaphlan2_db_choice.metaphlan2_db_selector == "cached"
+ #set $table = dict([(_[0], _[2]) for _ in $taxonomic_profile.metaphlan2_db_choice.cached_metaphlan2_db.input.options.tool_data_table.data])
+ #set $db_choice = $taxonomic_profile.metaphlan2_db_choice.cached_metaphlan2_db.value
#set $metaphlan_option += " --bowtie2db " + $table[$db_choice]
#set $metaphlan_option += " --mpa_pkl " + $table[$db_choice] + ".pkl"
#else
@@ -66,7 +68,6 @@
#set $metaphlan_option += " --mpa_pkl " + ref_db/metadata.pkl
#end if
#end if
-
--metaphlan-options="$metaphlan_option"
--evalue $e_value
@@ -110,11 +111,10 @@
-
+
-
+
@@ -122,7 +122,7 @@
-
+
diff -r 7838137cbc7f -r b0cd2569ccfb transform_json_to_pkl.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/transform_json_to_pkl.py Thu Jun 02 04:21:54 2016 -0400
@@ -0,0 +1,28 @@
+#!/usr/bin/env python
+# -*- coding: utf-8 -*-
+
+import cPickle as pickle
+import bz2
+import json
+import argparse
+
+def transform_json_to_pkl(args):
+ with open(args.json_input, 'r') as json_file:
+ json_str = json_file.read()
+ metadata = json.loads(json_str)
+
+ for marker in metadata["markers"]:
+ metadata["markers"][marker]["ext"] = set(metadata["markers"][marker]["ext"])
+
+ pkl_output = bz2.BZ2File(args.pkl_output, 'w')
+ pickle.dump(metadata, pkl_output, pickle.HIGHEST_PROTOCOL)
+ pkl_output.close()
+
+if __name__ == '__main__':
+ parser = argparse.ArgumentParser()
+ parser.add_argument('--json_input', required=True)
+ parser.add_argument('--pkl_output', required=True)
+
+ args = parser.parse_args()
+
+ transform_json_to_pkl(args)