# HG changeset patch # User bebatut # Date 1488541869 18000 # Node ID 56e8ce2f3c96daebd5356e9827184e716b0a10b3 # Parent 8b86d590b4428c67f817c865da88936f671fb752 planemo upload for repository https://github.com/ASaiM/galaxytools/tree/master/tools/humann2/ commit b'f45f31529390153bdaf93a3c94a65f6888ed484b\n'-dirty diff -r 8b86d590b442 -r 56e8ce2f3c96 README.rst --- a/README.rst Fri Mar 03 06:40:48 2017 -0500 +++ b/README.rst Fri Mar 03 06:51:09 2017 -0500 @@ -4,11 +4,9 @@ Galaxy should be able to automatically install the dependencies, i.e. the HUMAnN2 binaries and its dependencies. -After installation, you must tell Galaxy about the defaut database with -clade-specific marker genes used for taxonomic profiling (MetaPhlAn2), and where -to find it: +After installation, you must tell Galaxy about the defaut databases for MetaPhlAn2 and HUMAnN2. +You need to use the data managers for that and installing: -* Put the ``metaphlan2_db.loc`` file in the ``tool-data/`` folder, after uncommenting last line -* Download whole MetaPhlan2 source code: https://bitbucket.org/biobakery/metaphlan2/get/2.5.0.zip -* Unzip it -* Move ``db_v20`` folder into ``dependency_dir/metaphlan2/2.5.0/bebatut/package_metaphlan2_2_5_0/...`` folder +- MetaPhlAn2 database +- HUMAnN2 nucleotide database +- HUMAnN2 protein database \ No newline at end of file diff -r 8b86d590b442 -r 56e8ce2f3c96 humann2.xml --- a/humann2.xml Fri Mar 03 06:40:48 2017 -0500 +++ b/humann2.xml Fri Mar 03 06:51:09 2017 -0500 @@ -59,21 +59,21 @@ $bypass_nucleotide_search --annotation-gene-index $annotation_gene_index - #set $metaphlan_option = '-t rel_ab' + #set $metaphlan_option = "-t rel_ab" #if $taxo_profile.taxonomic_profile_test == "true": --taxonomic-profile '$taxo_profile.taxonomic_profile' #else #if $taxo_profile.metaphlan2_db_choice.metaphlan2_db_selector == "history" - #set $metaphlan_option += ' --bowtie2db ' + ref_db/ref_db - #set $metaphlan_option += ' --mpa_pkl ' + ref_db/metadata.pkl + #set $metaphlan_option += " --bowtie2db " + ref_db/ref_db + #set $metaphlan_option += " --mpa_pkl " + ref_db/metadata.pkl #else #set $path = $taxo_profile.metaphlan2_db_choice.cached_db.fields.path #set $value = $taxo_profile.metaphlan2_db_choice.cached_db.fields.value - #set $metaphlan_option += ' --bowtie2db ' + $path/$value - #set $metaphlan_option += ' --mpa_pkl ' + $path/$value + '.pkl' + #set $metaphlan_option += " --bowtie2db " + $path/$value + #set $metaphlan_option += " --mpa_pkl " + $path/$value + ".pkl" #end if #end if - --metaphlan-options='$metaphlan_option' + --metaphlan-options="$metaphlan_option" #if $nucleotide_db.nucleotide_db_selector == "cached" --nucleotide-database '$nucleotide_db.nucleotide_database.fields.path'