Mercurial > repos > artbio > small_rna_map
diff small_rna_map.xml @ 0:1ad5d040f85f draft
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_map commit fa452d860cf550c7524df59e77b36fd39e3e2a45
| author | artbio |
|---|---|
| date | Mon, 10 Jul 2017 10:31:49 -0400 |
| parents | |
| children | 2299eb1e7c93 |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/small_rna_map.xml Mon Jul 10 10:31:49 2017 -0400 @@ -0,0 +1,67 @@ +<tool id="small_rna_map" name="small rna map" version="0.1.0"> + <description></description> + <requirements> + <requirement type="package" version="1.11.2=py27_0">numpy</requirement> + <requirement type="package" version="0.11.2.1=py27_0">pysam</requirement> + <requirement type="package" version="1.3.2=r3.3.1_0">r-optparse</requirement> + <requirement type="package" version="2.2.1=r3.3.1_0">r-ggplot2</requirement> + <requirement type="package" version="2.2.1=r3.3.1_0">r-gridextra</requirement> + </requirements> + <stdio> + <exit_code range="1:" level="fatal" description="Tool exception" /> + </stdio> + <command detect_errors="exit_code"><![CDATA[ + python '$__tool_directory__'/small_rna_map.py + --input + #for $file in $input + '$file' + #end for + --output '$output_tab' && + Rscript '$__tool_directory__'/small_rna_map.r --output_tab '$output_tab' --output_pdf '$output_pdf' + ]]></command> + <inputs> + <param name="input" type="data" format="bam" label="Select multiple alignments to parse" multiple="True"/> + </inputs> + + <outputs> + <data format="tabular" name="output_tab" label="tabular file" /> + <data format="pdf" name="output_pdf" label="PDF file" /> +</outputs> + + <tests> + <test> + <param name="input" value="input1.bam,input2.bam" ftype="bam"/> + <output file="output.tab" name="output_tab" /> + <output file="output.pdf" name="output_pdf" /> + </test> + </tests> + + +<help> + +**What it does** + +Generate read count maps from alignment BAM files, using pysam and ggplot. +In addition to the read counts (bars), median size and mean size of reads mapping at a given position are plotted in the graphs. + +**Inputs** +Bam alignment files. Be carreful to select Bam corresponding to alignments to the same reference + +**Output** +A pdf file generated by the R package ggplot2 + +</help> + +<citations> + <citation type="doi">10.1093/bioinformatics/btp352</citation> + <citation type="bibtex">@Book{, + author = {Hadley Wickham}, + title = {ggplot2: Elegant Graphics for Data Analysis}, + publisher = {Springer-Verlag New York}, + year = {2009}, + isbn = {978-0-387-98140-6}, + url = {http://ggplot2.org}, + }</citation> +</citations> +</tool> +
